Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2170 and Pf1N1B4_2171 overlap by 4 nucleotides Pf1N1B4_2171 and Pf1N1B4_2172 are separated by 2 nucleotides Pf1N1B4_2172 and Pf1N1B4_2173 are separated by 13 nucleotides Pf1N1B4_2173 and Pf1N1B4_2174 are separated by 4 nucleotides
Pf1N1B4_2170 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 2,483,621 to 2,484,322
_2170
Pf1N1B4_2171 - Tyrosine recombinase XerC, at 2,484,319 to 2,485,218
_2171
Pf1N1B4_2172 - Protein of unknown function DUF484, at 2,485,221 to 2,485,946
_2172
Pf1N1B4_2173 - Diaminopimelate epimerase (EC 5.1.1.7), at 2,485,960 to 2,486,790
_2173
Pf1N1B4_2174 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 2,486,795 to 2,488,042
_2174
Position (kb)
2485
2486 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2484.336 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.368 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.404 kb on + strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.405 kb on - strand at 2484.797 kb on + strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.798 kb on - strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.967 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.977 kb on + strand, within Pf1N1B4_2171 at 2484.978 kb on - strand, within Pf1N1B4_2171 at 2485.148 kb on + strand at 2485.227 kb on + strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.286 kb on - strand at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.318 kb on + strand, within Pf1N1B4_2172 at 2485.319 kb on - strand at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.319 kb on - strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.626 kb on + strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.627 kb on - strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.821 kb on + strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.822 kb on - strand, within Pf1N1B4_2172 at 2485.918 kb on - strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.982 kb on + strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2485.983 kb on - strand at 2486.071 kb on + strand, within Pf1N1B4_2173 at 2486.369 kb on + strand, within Pf1N1B4_2173 at 2486.369 kb on + strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.370 kb on - strand, within Pf1N1B4_2173 at 2486.796 kb on + strand at 2486.797 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,484,336 + +0.3 2,484,368 + -1.3 2,484,368 + +1.5 2,484,368 + -0.7 2,484,404 + +1.2 2,484,404 + -0.1 2,484,404 + -1.1 2,484,404 + +1.1 2,484,404 + +0.5 2,484,405 - -0.8 2,484,405 - +1.5 2,484,405 - +1.5 2,484,797 + Pf1N1B4_2171 0.53 -1.5 2,484,798 - Pf1N1B4_2171 0.53 +2.5 2,484,798 - Pf1N1B4_2171 0.53 -0.1 2,484,967 + Pf1N1B4_2171 0.72 +0.7 2,484,967 + Pf1N1B4_2171 0.72 -0.5 2,484,967 + Pf1N1B4_2171 0.72 -1.0 2,484,967 + Pf1N1B4_2171 0.72 +1.2 2,484,977 + Pf1N1B4_2171 0.73 -0.5 2,484,977 + Pf1N1B4_2171 0.73 +3.1 2,484,978 - Pf1N1B4_2171 0.73 +0.2 2,485,148 + +0.5 2,485,227 + -0.1 2,485,286 - -1.3 2,485,286 - +0.9 2,485,286 - +0.9 2,485,286 - -1.3 2,485,286 - +1.2 2,485,318 + Pf1N1B4_2172 0.13 +0.2 2,485,318 + Pf1N1B4_2172 0.13 -0.8 2,485,318 + Pf1N1B4_2172 0.13 +0.9 2,485,318 + Pf1N1B4_2172 0.13 +1.2 2,485,319 - +1.5 2,485,319 - Pf1N1B4_2172 0.13 +0.2 2,485,319 - Pf1N1B4_2172 0.13 -0.7 2,485,319 - Pf1N1B4_2172 0.13 +1.6 2,485,626 + Pf1N1B4_2172 0.56 +0.3 2,485,626 + Pf1N1B4_2172 0.56 +0.8 2,485,627 - Pf1N1B4_2172 0.56 +0.3 2,485,627 - Pf1N1B4_2172 0.56 +1.9 2,485,627 - Pf1N1B4_2172 0.56 +1.3 2,485,821 + Pf1N1B4_2172 0.83 +0.5 2,485,821 + Pf1N1B4_2172 0.83 -2.1 2,485,821 + Pf1N1B4_2172 0.83 +0.3 2,485,822 - Pf1N1B4_2172 0.83 +0.9 2,485,822 - Pf1N1B4_2172 0.83 +0.9 2,485,822 - Pf1N1B4_2172 0.83 +1.5 2,485,918 - +0.5 2,485,982 + +0.6 2,485,982 + +1.5 2,485,982 + -0.8 2,485,982 + +0.3 2,485,983 - +0.5 2,485,983 - +0.9 2,485,983 - +2.5 2,486,071 + Pf1N1B4_2173 0.13 +0.5 2,486,369 + Pf1N1B4_2173 0.49 -1.2 2,486,369 + Pf1N1B4_2173 0.49 +1.3 2,486,370 - Pf1N1B4_2173 0.49 +0.5 2,486,370 - Pf1N1B4_2173 0.49 -0.1 2,486,370 - Pf1N1B4_2173 0.49 -0.3 2,486,370 - Pf1N1B4_2173 0.49 +0.6 2,486,796 + +0.7 2,486,797 - +0.2
Or see this region's nucleotide sequence