Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2101 and Pf1N1B4_2102 are separated by 128 nucleotides Pf1N1B4_2102 and Pf1N1B4_2103 are separated by 588 nucleotides
Pf1N1B4_2101 - FIG00953563: hypothetical protein, at 2,412,616 to 2,413,977
_2101
Pf1N1B4_2102 - hypothetical protein, at 2,414,106 to 2,414,486
_2102
Pf1N1B4_2103 - Superfamily II DNA and RNA helicases, at 2,415,075 to 2,415,452
_2103
Position (kb)
2414
2415 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2413.151 kb on + strand, within Pf1N1B4_2101 at 2413.200 kb on + strand, within Pf1N1B4_2101 at 2413.344 kb on + strand, within Pf1N1B4_2101 at 2413.487 kb on + strand, within Pf1N1B4_2101 at 2413.488 kb on - strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.546 kb on + strand, within Pf1N1B4_2101 at 2413.547 kb on - strand, within Pf1N1B4_2101 at 2413.547 kb on - strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.550 kb on + strand, within Pf1N1B4_2101 at 2413.551 kb on - strand, within Pf1N1B4_2101 at 2413.551 kb on - strand, within Pf1N1B4_2101 at 2413.729 kb on + strand, within Pf1N1B4_2101 at 2413.729 kb on + strand, within Pf1N1B4_2101 at 2413.730 kb on - strand, within Pf1N1B4_2101 at 2413.730 kb on - strand, within Pf1N1B4_2101 at 2413.773 kb on + strand, within Pf1N1B4_2101 at 2413.809 kb on + strand, within Pf1N1B4_2101 at 2413.810 kb on - strand, within Pf1N1B4_2101 at 2413.810 kb on - strand, within Pf1N1B4_2101 at 2413.813 kb on + strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.814 kb on - strand, within Pf1N1B4_2101 at 2413.857 kb on + strand at 2413.857 kb on + strand at 2413.857 kb on + strand at 2413.858 kb on - strand at 2413.858 kb on - strand at 2413.955 kb on + strand at 2413.955 kb on + strand at 2413.955 kb on + strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.956 kb on - strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.993 kb on + strand at 2413.994 kb on - strand at 2413.994 kb on - strand at 2413.994 kb on - strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.995 kb on + strand at 2413.996 kb on - strand at 2413.996 kb on - strand at 2414.032 kb on + strand at 2414.032 kb on + strand at 2414.093 kb on - strand at 2414.107 kb on + strand at 2414.108 kb on - strand at 2414.108 kb on - strand at 2414.147 kb on + strand, within Pf1N1B4_2102 at 2414.147 kb on + strand, within Pf1N1B4_2102 at 2414.148 kb on - strand, within Pf1N1B4_2102 at 2414.148 kb on - strand, within Pf1N1B4_2102 at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.492 kb on + strand at 2414.493 kb on - strand at 2414.504 kb on + strand at 2414.504 kb on + strand at 2414.504 kb on + strand at 2414.505 kb on - strand at 2414.505 kb on - strand at 2414.511 kb on + strand at 2414.511 kb on + strand at 2414.511 kb on + strand at 2414.512 kb on - strand at 2414.553 kb on + strand at 2414.553 kb on + strand at 2414.553 kb on + strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.554 kb on - strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.555 kb on + strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.556 kb on - strand at 2414.634 kb on + strand at 2414.634 kb on + strand at 2414.634 kb on + strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.635 kb on - strand at 2414.653 kb on + strand at 2414.654 kb on - strand at 2414.750 kb on + strand at 2414.750 kb on + strand at 2414.750 kb on + strand at 2414.751 kb on - strand at 2414.751 kb on - strand at 2414.751 kb on - strand at 2414.839 kb on - strand at 2414.839 kb on - strand at 2414.862 kb on + strand at 2414.862 kb on + strand at 2414.995 kb on + strand at 2415.034 kb on - strand at 2415.134 kb on + strand, within Pf1N1B4_2103 at 2415.134 kb on + strand, within Pf1N1B4_2103 at 2415.135 kb on - strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.184 kb on + strand, within Pf1N1B4_2103 at 2415.185 kb on - strand, within Pf1N1B4_2103 at 2415.239 kb on + strand, within Pf1N1B4_2103 at 2415.240 kb on - strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.258 kb on + strand, within Pf1N1B4_2103 at 2415.259 kb on - strand, within Pf1N1B4_2103 at 2415.259 kb on - strand, within Pf1N1B4_2103 at 2415.344 kb on + strand, within Pf1N1B4_2103 at 2415.344 kb on + strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.345 kb on - strand, within Pf1N1B4_2103 at 2415.423 kb on + strand at 2415.423 kb on + strand at 2415.423 kb on + strand at 2415.424 kb on - strand at 2415.434 kb on + strand at 2415.434 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,413,151 + Pf1N1B4_2101 0.39 +0.4 2,413,200 + Pf1N1B4_2101 0.43 +0.1 2,413,344 + Pf1N1B4_2101 0.53 +0.8 2,413,487 + Pf1N1B4_2101 0.64 -0.5 2,413,488 - Pf1N1B4_2101 0.64 -0.1 2,413,546 + Pf1N1B4_2101 0.68 -0.1 2,413,546 + Pf1N1B4_2101 0.68 +0.5 2,413,546 + Pf1N1B4_2101 0.68 +1.0 2,413,547 - Pf1N1B4_2101 0.68 +1.2 2,413,547 - Pf1N1B4_2101 0.68 +1.7 2,413,550 + Pf1N1B4_2101 0.69 -0.1 2,413,550 + Pf1N1B4_2101 0.69 +0.7 2,413,550 + Pf1N1B4_2101 0.69 +0.8 2,413,550 + Pf1N1B4_2101 0.69 +0.2 2,413,550 + Pf1N1B4_2101 0.69 -0.5 2,413,550 + Pf1N1B4_2101 0.69 +0.3 2,413,550 + Pf1N1B4_2101 0.69 -0.7 2,413,550 + Pf1N1B4_2101 0.69 -0.2 2,413,550 + Pf1N1B4_2101 0.69 -0.4 2,413,550 + Pf1N1B4_2101 0.69 -1.0 2,413,550 + Pf1N1B4_2101 0.69 -1.0 2,413,550 + Pf1N1B4_2101 0.69 -0.8 2,413,550 + Pf1N1B4_2101 0.69 +0.8 2,413,550 + Pf1N1B4_2101 0.69 -0.4 2,413,550 + +1.5 2,413,550 + Pf1N1B4_2101 0.69 +0.5 2,413,550 + Pf1N1B4_2101 0.69 -2.0 2,413,551 - Pf1N1B4_2101 0.69 +1.5 2,413,551 - Pf1N1B4_2101 0.69 -0.1 2,413,729 + Pf1N1B4_2101 0.82 +0.6 2,413,729 + Pf1N1B4_2101 0.82 +0.8 2,413,730 - Pf1N1B4_2101 0.82 -1.1 2,413,730 - Pf1N1B4_2101 0.82 -1.1 2,413,773 + Pf1N1B4_2101 0.85 -0.1 2,413,809 + Pf1N1B4_2101 0.88 +1.0 2,413,810 - Pf1N1B4_2101 0.88 +1.5 2,413,810 - Pf1N1B4_2101 0.88 +0.5 2,413,813 + Pf1N1B4_2101 0.88 +2.8 2,413,814 - Pf1N1B4_2101 0.88 -0.2 2,413,814 - Pf1N1B4_2101 0.88 +0.4 2,413,814 - Pf1N1B4_2101 0.88 +1.1 2,413,814 - Pf1N1B4_2101 0.88 -1.2 2,413,857 + +2.1 2,413,857 + +0.1 2,413,857 + -1.6 2,413,858 - -1.5 2,413,858 - -0.8 2,413,955 + +1.5 2,413,955 + -0.1 2,413,955 + +0.8 2,413,956 - -1.1 2,413,956 - +0.5 2,413,956 - -0.3 2,413,956 - +0.5 2,413,956 - -0.4 2,413,956 - +1.0 2,413,993 + -0.2 2,413,993 + -0.2 2,413,993 + +0.5 2,413,993 + -1.5 2,413,993 + -0.8 2,413,994 - +0.8 2,413,994 - +3.1 2,413,994 - -0.1 2,413,995 + -1.5 2,413,995 + -0.4 2,413,995 + -1.3 2,413,995 + +0.2 2,413,995 + -0.7 2,413,995 + -1.5 2,413,995 + +0.9 2,413,996 - -1.2 2,413,996 - -1.1 2,414,032 + +1.1 2,414,032 + +0.4 2,414,093 - -0.8 2,414,107 + +0.5 2,414,108 - +0.1 2,414,108 - -0.4 2,414,147 + Pf1N1B4_2102 0.11 +0.1 2,414,147 + Pf1N1B4_2102 0.11 -0.5 2,414,148 - Pf1N1B4_2102 0.11 -0.5 2,414,148 - Pf1N1B4_2102 0.11 -0.6 2,414,492 + -1.8 2,414,492 + -0.1 2,414,492 + +1.5 2,414,492 + -0.3 2,414,492 + -0.8 2,414,492 + -0.8 2,414,493 - +0.5 2,414,504 + -0.6 2,414,504 + +0.3 2,414,504 + +0.2 2,414,505 - +2.1 2,414,505 - +0.1 2,414,511 + -0.5 2,414,511 + +0.8 2,414,511 + +0.4 2,414,512 - -0.5 2,414,553 + +0.4 2,414,553 + -1.7 2,414,553 + -0.6 2,414,554 - +0.6 2,414,554 - -0.5 2,414,554 - +0.8 2,414,554 - +0.8 2,414,554 - +1.5 2,414,555 + -1.5 2,414,555 + +1.9 2,414,555 + -1.0 2,414,555 + +1.3 2,414,555 + +0.5 2,414,555 + -0.8 2,414,555 + -1.1 2,414,555 + -0.3 2,414,555 + +0.5 2,414,555 + +0.8 2,414,555 + +1.7 2,414,555 + -1.0 2,414,555 + -2.0 2,414,555 + -0.5 2,414,555 + +0.5 2,414,556 - +0.5 2,414,556 - +0.8 2,414,556 - -0.3 2,414,556 - +0.5 2,414,556 - -0.1 2,414,556 - +1.5 2,414,556 - +0.8 2,414,556 - -1.7 2,414,556 - -1.1 2,414,556 - +0.3 2,414,556 - -1.4 2,414,556 - +0.8 2,414,556 - -1.5 2,414,556 - -1.3 2,414,556 - -2.6 2,414,556 - -1.8 2,414,556 - +0.8 2,414,556 - -1.9 2,414,556 - -2.1 2,414,556 - +1.5 2,414,556 - -1.5 2,414,556 - -1.3 2,414,556 - -1.3 2,414,556 - -0.8 2,414,556 - +0.3 2,414,556 - -0.5 2,414,634 + +0.5 2,414,634 + -0.7 2,414,634 + -0.8 2,414,635 - -1.7 2,414,635 - +0.3 2,414,635 - +0.3 2,414,635 - -2.1 2,414,653 + -1.3 2,414,654 - +3.1 2,414,750 + -0.1 2,414,750 + -0.4 2,414,750 + -0.2 2,414,751 - +1.5 2,414,751 - -0.8 2,414,751 - +1.2 2,414,839 - -0.5 2,414,839 - -0.8 2,414,862 + +0.6 2,414,862 + -1.1 2,414,995 + -0.1 2,415,034 - +0.1 2,415,134 + Pf1N1B4_2103 0.16 -0.6 2,415,134 + Pf1N1B4_2103 0.16 +0.5 2,415,135 - Pf1N1B4_2103 0.16 -1.0 2,415,184 + Pf1N1B4_2103 0.29 -1.7 2,415,184 + Pf1N1B4_2103 0.29 -1.2 2,415,184 + Pf1N1B4_2103 0.29 +0.5 2,415,184 + Pf1N1B4_2103 0.29 -1.7 2,415,185 - Pf1N1B4_2103 0.29 -0.1 2,415,239 + Pf1N1B4_2103 0.43 -0.3 2,415,240 - Pf1N1B4_2103 0.44 -0.3 2,415,258 + Pf1N1B4_2103 0.48 -1.3 2,415,258 + Pf1N1B4_2103 0.48 +1.1 2,415,258 + Pf1N1B4_2103 0.48 -0.9 2,415,258 + Pf1N1B4_2103 0.48 +0.1 2,415,258 + Pf1N1B4_2103 0.48 +0.1 2,415,258 + Pf1N1B4_2103 0.48 +1.5 2,415,258 + Pf1N1B4_2103 0.48 -0.2 2,415,258 + Pf1N1B4_2103 0.48 +1.5 2,415,259 - Pf1N1B4_2103 0.49 -0.2 2,415,259 - Pf1N1B4_2103 0.49 +0.3 2,415,344 + Pf1N1B4_2103 0.71 -0.5 2,415,344 + Pf1N1B4_2103 0.71 -0.1 2,415,345 - Pf1N1B4_2103 0.71 +0.7 2,415,345 - Pf1N1B4_2103 0.71 -1.7 2,415,345 - Pf1N1B4_2103 0.71 -0.5 2,415,423 + +1.5 2,415,423 + -0.3 2,415,423 + +0.5 2,415,424 - -0.7 2,415,434 + +0.3 2,415,434 + +0.3
Or see this region's nucleotide sequence