Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1945 and Pf1N1B4_1946 are separated by 204 nucleotides Pf1N1B4_1946 and Pf1N1B4_1947 are separated by 1 nucleotides
Pf1N1B4_1945 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 2,225,060 to 2,226,427
_1945
Pf1N1B4_1946 - Transcriptional regulator of glmS gene, DeoR family, at 2,226,632 to 2,227,408
_1946
Pf1N1B4_1947 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 2,227,410 to 2,229,242
_1947
Position (kb)
2226
2227
2228 Strain fitness (log2 ratio)
-2
-1
0
1 at 2226.430 kb on + strand at 2226.496 kb on - strand at 2226.860 kb on + strand, within Pf1N1B4_1946 at 2226.860 kb on + strand, within Pf1N1B4_1946 at 2226.860 kb on + strand, within Pf1N1B4_1946 at 2227.001 kb on + strand, within Pf1N1B4_1946 at 2227.001 kb on + strand, within Pf1N1B4_1946 at 2227.001 kb on + strand, within Pf1N1B4_1946 at 2227.001 kb on + strand, within Pf1N1B4_1946 at 2227.110 kb on + strand, within Pf1N1B4_1946 at 2227.110 kb on + strand, within Pf1N1B4_1946 at 2227.113 kb on + strand, within Pf1N1B4_1946 at 2227.121 kb on + strand, within Pf1N1B4_1946 at 2227.121 kb on + strand, within Pf1N1B4_1946 at 2227.137 kb on + strand, within Pf1N1B4_1946 at 2227.137 kb on + strand, within Pf1N1B4_1946 at 2227.137 kb on + strand, within Pf1N1B4_1946 at 2227.309 kb on + strand, within Pf1N1B4_1946 at 2227.318 kb on + strand, within Pf1N1B4_1946 at 2227.372 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 2,226,430 + +1.6 2,226,496 - -1.8 2,226,860 + Pf1N1B4_1946 0.29 -1.3 2,226,860 + Pf1N1B4_1946 0.29 +0.2 2,226,860 + Pf1N1B4_1946 0.29 -2.1 2,227,001 + Pf1N1B4_1946 0.47 -1.2 2,227,001 + Pf1N1B4_1946 0.47 -0.2 2,227,001 + Pf1N1B4_1946 0.47 -0.8 2,227,001 + Pf1N1B4_1946 0.47 +0.3 2,227,110 + Pf1N1B4_1946 0.62 +1.0 2,227,110 + Pf1N1B4_1946 0.62 +1.3 2,227,113 + Pf1N1B4_1946 0.62 +0.6 2,227,121 + Pf1N1B4_1946 0.63 -2.4 2,227,121 + Pf1N1B4_1946 0.63 -0.2 2,227,137 + Pf1N1B4_1946 0.65 -1.8 2,227,137 + Pf1N1B4_1946 0.65 -2.0 2,227,137 + Pf1N1B4_1946 0.65 -0.4 2,227,309 + Pf1N1B4_1946 0.87 -0.4 2,227,318 + Pf1N1B4_1946 0.88 -0.8 2,227,372 + -1.8
Or see this region's nucleotide sequence