Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1741 and Pf1N1B4_1742 are separated by 178 nucleotides Pf1N1B4_1742 and Pf1N1B4_1743 overlap by 4 nucleotides Pf1N1B4_1743 and Pf1N1B4_1744 are separated by 42 nucleotides
Pf1N1B4_1741 - Ferredoxin reductase, at 1,989,831 to 1,991,360
_1741
Pf1N1B4_1742 - Biofilm PGA synthesis auxiliary protein PgaD, at 1,991,539 to 1,992,066
_1742
Pf1N1B4_1743 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 1,992,063 to 1,993,418
_1743
Pf1N1B4_1744 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 1,993,461 to 1,995,416
_1744
Position (kb)
1992
1993
1994 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1991.104 kb on - strand, within Pf1N1B4_1741 at 1991.118 kb on + strand, within Pf1N1B4_1741 at 1991.149 kb on - strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.154 kb on + strand, within Pf1N1B4_1741 at 1991.387 kb on - strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.470 kb on + strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.471 kb on - strand at 1991.473 kb on - strand at 1991.491 kb on + strand at 1991.491 kb on + strand at 1991.540 kb on + strand at 1991.540 kb on + strand at 1991.541 kb on - strand at 1991.541 kb on - strand at 1991.629 kb on + strand, within Pf1N1B4_1742 at 1991.629 kb on + strand at 1991.694 kb on + strand, within Pf1N1B4_1742 at 1991.886 kb on - strand at 1991.886 kb on - strand, within Pf1N1B4_1742 at 1991.886 kb on - strand, within Pf1N1B4_1742 at 1991.977 kb on + strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.978 kb on - strand, within Pf1N1B4_1742 at 1991.981 kb on + strand, within Pf1N1B4_1742 at 1991.981 kb on + strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1991.982 kb on - strand, within Pf1N1B4_1742 at 1992.183 kb on - strand at 1992.183 kb on - strand at 1992.895 kb on + strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743 at 1992.896 kb on - strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.168 kb on + strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.169 kb on - strand, within Pf1N1B4_1743 at 1993.223 kb on + strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.224 kb on - strand, within Pf1N1B4_1743 at 1993.267 kb on + strand, within Pf1N1B4_1743 at 1993.268 kb on - strand, within Pf1N1B4_1743 at 1993.401 kb on + strand at 1993.402 kb on - strand at 1993.421 kb on + strand at 1993.421 kb on + strand at 1993.500 kb on - strand at 1993.737 kb on - strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.827 kb on + strand, within Pf1N1B4_1744 at 1993.828 kb on - strand, within Pf1N1B4_1744 at 1993.975 kb on + strand, within Pf1N1B4_1744 at 1994.036 kb on - strand, within Pf1N1B4_1744 at 1994.036 kb on - strand, within Pf1N1B4_1744 at 1994.077 kb on + strand, within Pf1N1B4_1744 at 1994.077 kb on + strand, within Pf1N1B4_1744 at 1994.131 kb on + strand, within Pf1N1B4_1744 at 1994.131 kb on + strand, within Pf1N1B4_1744 at 1994.132 kb on - strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.233 kb on + strand, within Pf1N1B4_1744 at 1994.285 kb on + strand, within Pf1N1B4_1744 at 1994.320 kb on + strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744 at 1994.321 kb on - strand, within Pf1N1B4_1744
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 1,991,104 - Pf1N1B4_1741 0.83 +0.7 1,991,118 + Pf1N1B4_1741 0.84 +0.0 1,991,149 - Pf1N1B4_1741 0.86 -1.5 1,991,154 + Pf1N1B4_1741 0.86 -0.5 1,991,154 + Pf1N1B4_1741 0.86 +0.9 1,991,154 + Pf1N1B4_1741 0.86 -0.4 1,991,387 - +0.2 1,991,470 + +1.5 1,991,470 + -0.5 1,991,470 + -0.6 1,991,470 + -0.1 1,991,471 - +1.5 1,991,471 - -0.6 1,991,471 - +0.9 1,991,471 - +0.3 1,991,473 - -0.3 1,991,491 + +1.8 1,991,491 + +1.2 1,991,540 + +0.4 1,991,540 + +1.9 1,991,541 - -0.5 1,991,541 - +1.1 1,991,629 + Pf1N1B4_1742 0.17 +0.9 1,991,629 + +0.5 1,991,694 + Pf1N1B4_1742 0.29 +0.7 1,991,886 - +0.5 1,991,886 - Pf1N1B4_1742 0.66 +0.6 1,991,886 - Pf1N1B4_1742 0.66 +0.5 1,991,977 + Pf1N1B4_1742 0.83 +1.5 1,991,978 - Pf1N1B4_1742 0.83 -1.8 1,991,978 - Pf1N1B4_1742 0.83 -2.2 1,991,978 - Pf1N1B4_1742 0.83 -0.2 1,991,978 - Pf1N1B4_1742 0.83 +0.7 1,991,978 - Pf1N1B4_1742 0.83 -1.3 1,991,981 + Pf1N1B4_1742 0.84 -1.3 1,991,981 + Pf1N1B4_1742 0.84 +0.2 1,991,982 - Pf1N1B4_1742 0.84 -0.5 1,991,982 - Pf1N1B4_1742 0.84 -1.3 1,991,982 - Pf1N1B4_1742 0.84 -1.3 1,991,982 - Pf1N1B4_1742 0.84 +1.3 1,991,982 - Pf1N1B4_1742 0.84 +1.5 1,992,183 - -1.7 1,992,183 - -1.9 1,992,895 + Pf1N1B4_1743 0.61 -0.8 1,992,896 - Pf1N1B4_1743 0.61 -0.9 1,992,896 - Pf1N1B4_1743 0.61 -1.1 1,993,168 + Pf1N1B4_1743 0.81 -0.4 1,993,168 + Pf1N1B4_1743 0.81 -1.7 1,993,168 + Pf1N1B4_1743 0.81 +0.2 1,993,168 + Pf1N1B4_1743 0.81 -1.8 1,993,168 + Pf1N1B4_1743 0.81 -1.1 1,993,169 - Pf1N1B4_1743 0.82 +1.5 1,993,169 - Pf1N1B4_1743 0.82 -0.8 1,993,169 - Pf1N1B4_1743 0.82 -0.3 1,993,169 - Pf1N1B4_1743 0.82 -1.2 1,993,223 + Pf1N1B4_1743 0.86 +0.3 1,993,224 - Pf1N1B4_1743 0.86 -0.2 1,993,224 - Pf1N1B4_1743 0.86 -1.1 1,993,224 - Pf1N1B4_1743 0.86 -1.3 1,993,267 + Pf1N1B4_1743 0.89 -1.3 1,993,268 - Pf1N1B4_1743 0.89 -0.6 1,993,401 + -0.5 1,993,402 - +1.2 1,993,421 + -1.7 1,993,421 + -1.3 1,993,500 - -0.8 1,993,737 - Pf1N1B4_1744 0.14 +0.5 1,993,827 + Pf1N1B4_1744 0.19 +2.5 1,993,827 + Pf1N1B4_1744 0.19 -1.3 1,993,827 + Pf1N1B4_1744 0.19 +1.1 1,993,828 - Pf1N1B4_1744 0.19 +0.8 1,993,975 + Pf1N1B4_1744 0.26 +1.5 1,994,036 - Pf1N1B4_1744 0.29 -0.4 1,994,036 - Pf1N1B4_1744 0.29 -0.2 1,994,077 + Pf1N1B4_1744 0.31 +1.9 1,994,077 + Pf1N1B4_1744 0.31 -0.1 1,994,131 + Pf1N1B4_1744 0.34 -1.5 1,994,131 + Pf1N1B4_1744 0.34 -0.3 1,994,132 - Pf1N1B4_1744 0.34 +0.2 1,994,233 + Pf1N1B4_1744 0.39 +2.5 1,994,233 + Pf1N1B4_1744 0.39 +1.1 1,994,233 + Pf1N1B4_1744 0.39 -0.1 1,994,285 + Pf1N1B4_1744 0.42 -0.5 1,994,320 + Pf1N1B4_1744 0.44 -1.1 1,994,321 - Pf1N1B4_1744 0.44 -0.5 1,994,321 - Pf1N1B4_1744 0.44 -0.8 1,994,321 - Pf1N1B4_1744 0.44 +1.4
Or see this region's nucleotide sequence