Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1663 and Pf1N1B4_1664 are separated by 886 nucleotides Pf1N1B4_1664 and Pf1N1B4_1665 are separated by 160 nucleotides
Pf1N1B4_1663 - 4-aminobutyrate aminotransferase (EC 2.6.1.19), at 1,904,166 to 1,907,078
_1663
Pf1N1B4_1664 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 1,907,965 to 1,908,510
_1664
Pf1N1B4_1665 - Signal transduction histidine kinase regulating C4-dicarboxylate transport system, at 1,908,671 to 1,910,425
_1665
Position (kb)
1907
1908
1909 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1907.079 kb on - strand at 1907.079 kb on - strand at 1907.098 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.109 kb on + strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.110 kb on - strand at 1907.111 kb on + strand at 1907.112 kb on - strand at 1907.112 kb on - strand at 1907.145 kb on + strand at 1907.146 kb on - strand at 1907.146 kb on - strand at 1907.147 kb on + strand at 1907.147 kb on + strand at 1907.148 kb on - strand at 1907.210 kb on + strand at 1907.210 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.253 kb on + strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.254 kb on - strand at 1907.428 kb on + strand at 1907.466 kb on - strand at 1907.466 kb on - strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.537 kb on + strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.538 kb on - strand at 1907.547 kb on + strand at 1907.547 kb on + strand at 1907.577 kb on - strand at 1907.586 kb on + strand at 1907.626 kb on + strand at 1907.626 kb on + strand at 1907.627 kb on - strand at 1907.627 kb on - strand at 1907.641 kb on + strand at 1907.642 kb on - strand at 1907.642 kb on - strand at 1907.677 kb on + strand at 1907.678 kb on - strand at 1907.785 kb on + strand at 1907.785 kb on + strand at 1907.785 kb on + strand at 1907.786 kb on - strand at 1907.786 kb on - strand at 1907.786 kb on - strand at 1907.803 kb on + strand at 1907.804 kb on - strand at 1907.848 kb on + strand at 1907.848 kb on + strand at 1907.926 kb on - strand at 1907.948 kb on - strand at 1907.948 kb on - strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.972 kb on + strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1907.973 kb on - strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.017 kb on + strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.018 kb on - strand at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.043 kb on + strand, within Pf1N1B4_1664 at 1908.044 kb on - strand, within Pf1N1B4_1664 at 1908.044 kb on - strand, within Pf1N1B4_1664 at 1908.134 kb on + strand, within Pf1N1B4_1664 at 1908.151 kb on + strand, within Pf1N1B4_1664 at 1908.355 kb on + strand, within Pf1N1B4_1664 at 1908.356 kb on - strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.370 kb on + strand, within Pf1N1B4_1664 at 1908.371 kb on - strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.373 kb on + strand, within Pf1N1B4_1664 at 1908.374 kb on - strand, within Pf1N1B4_1664 at 1908.374 kb on - strand, within Pf1N1B4_1664 at 1908.455 kb on + strand, within Pf1N1B4_1664 at 1908.455 kb on + strand, within Pf1N1B4_1664 at 1908.456 kb on - strand at 1908.456 kb on - strand at 1908.584 kb on + strand at 1908.584 kb on + strand at 1908.584 kb on + strand at 1908.585 kb on - strand at 1908.585 kb on - strand at 1908.593 kb on + strand at 1908.593 kb on + strand at 1908.594 kb on - strand at 1908.595 kb on + strand at 1908.596 kb on - strand at 1908.722 kb on + strand at 1908.800 kb on + strand at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.938 kb on + strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1908.939 kb on - strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.028 kb on + strand, within Pf1N1B4_1665 at 1909.029 kb on - strand, within Pf1N1B4_1665 at 1909.100 kb on + strand, within Pf1N1B4_1665 at 1909.101 kb on - strand, within Pf1N1B4_1665 at 1909.491 kb on + strand, within Pf1N1B4_1665 at 1909.491 kb on + strand, within Pf1N1B4_1665 at 1909.492 kb on - strand, within Pf1N1B4_1665
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 1,907,079 - +1.0 1,907,079 - +0.0 1,907,098 + -0.8 1,907,109 + -1.1 1,907,109 + -0.1 1,907,109 + +1.1 1,907,109 + +1.5 1,907,110 - -1.0 1,907,110 - -0.1 1,907,110 - +0.5 1,907,110 - +1.4 1,907,110 - +1.0 1,907,110 - +1.5 1,907,110 - +1.7 1,907,110 - +0.2 1,907,110 - -0.3 1,907,111 + -0.5 1,907,112 - +1.2 1,907,112 - -0.7 1,907,145 + -1.5 1,907,146 - +0.1 1,907,146 - -1.9 1,907,147 + -0.6 1,907,147 + +0.9 1,907,148 - +0.3 1,907,210 + +0.5 1,907,210 + +0.0 1,907,253 + +1.5 1,907,253 + +0.5 1,907,253 + -0.8 1,907,253 + +1.0 1,907,253 + +1.1 1,907,254 - +0.5 1,907,254 - -2.0 1,907,254 - +0.3 1,907,254 - +0.9 1,907,254 - +0.9 1,907,254 - +2.0 1,907,254 - -1.3 1,907,254 - -0.9 1,907,428 + -0.5 1,907,466 - -0.5 1,907,466 - +0.5 1,907,537 + +0.1 1,907,537 + +1.5 1,907,537 + +1.7 1,907,537 + +0.4 1,907,537 + -0.5 1,907,537 + -0.3 1,907,538 - -0.0 1,907,538 - -0.5 1,907,538 - +1.5 1,907,538 - -2.9 1,907,538 - +1.2 1,907,547 + -1.7 1,907,547 + -0.1 1,907,577 - -0.2 1,907,586 + +0.5 1,907,626 + +0.2 1,907,626 + +0.3 1,907,627 - +2.3 1,907,627 - +0.9 1,907,641 + -0.1 1,907,642 - +0.9 1,907,642 - +0.5 1,907,677 + +0.5 1,907,678 - +0.1 1,907,785 + -1.5 1,907,785 + -0.5 1,907,785 + -2.5 1,907,786 - -0.2 1,907,786 - +0.0 1,907,786 - +0.9 1,907,803 + +2.8 1,907,804 - -0.9 1,907,848 + +0.7 1,907,848 + +1.0 1,907,926 - +0.9 1,907,948 - +1.1 1,907,948 - -2.0 1,907,972 + +0.2 1,907,972 + +0.1 1,907,972 + +0.7 1,907,972 + +1.5 1,907,973 - +0.0 1,907,973 - -0.1 1,907,973 - -0.6 1,907,973 - -0.1 1,908,017 + +0.6 1,908,017 + -1.3 1,908,017 + +0.6 1,908,017 + +0.7 1,908,017 + +0.1 1,908,017 + -0.9 1,908,017 + +0.3 1,908,018 - +0.5 1,908,018 - +0.1 1,908,018 - -1.3 1,908,018 - -0.1 1,908,018 - -0.1 1,908,018 - +0.3 1,908,018 - -0.1 1,908,018 - +0.5 1,908,043 + Pf1N1B4_1664 0.14 +1.2 1,908,043 + Pf1N1B4_1664 0.14 +0.3 1,908,043 + Pf1N1B4_1664 0.14 +1.0 1,908,043 + Pf1N1B4_1664 0.14 +0.5 1,908,044 - Pf1N1B4_1664 0.14 -1.1 1,908,044 - Pf1N1B4_1664 0.14 +1.2 1,908,134 + Pf1N1B4_1664 0.31 -0.7 1,908,151 + Pf1N1B4_1664 0.34 -0.9 1,908,355 + Pf1N1B4_1664 0.71 +1.1 1,908,356 - Pf1N1B4_1664 0.72 +0.5 1,908,370 + Pf1N1B4_1664 0.74 -1.1 1,908,370 + Pf1N1B4_1664 0.74 +0.1 1,908,370 + Pf1N1B4_1664 0.74 +1.5 1,908,370 + Pf1N1B4_1664 0.74 +0.9 1,908,371 - Pf1N1B4_1664 0.74 -0.5 1,908,373 + Pf1N1B4_1664 0.75 -0.1 1,908,373 + Pf1N1B4_1664 0.75 -2.0 1,908,373 + Pf1N1B4_1664 0.75 +1.5 1,908,373 + Pf1N1B4_1664 0.75 +0.3 1,908,373 + Pf1N1B4_1664 0.75 +1.1 1,908,374 - Pf1N1B4_1664 0.75 +2.0 1,908,374 - Pf1N1B4_1664 0.75 -0.3 1,908,455 + Pf1N1B4_1664 0.90 -0.5 1,908,455 + Pf1N1B4_1664 0.90 -2.2 1,908,456 - -1.3 1,908,456 - -0.6 1,908,584 + +0.8 1,908,584 + -0.1 1,908,584 + +0.3 1,908,585 - -0.4 1,908,585 - +0.1 1,908,593 + +0.1 1,908,593 + +1.0 1,908,594 - +0.0 1,908,595 + -0.9 1,908,596 - +1.8 1,908,722 + +1.3 1,908,800 + -1.1 1,908,938 + Pf1N1B4_1665 0.15 +0.6 1,908,938 + Pf1N1B4_1665 0.15 -0.7 1,908,938 + Pf1N1B4_1665 0.15 -0.8 1,908,938 + Pf1N1B4_1665 0.15 -0.1 1,908,938 + Pf1N1B4_1665 0.15 +0.8 1,908,938 + Pf1N1B4_1665 0.15 +1.0 1,908,938 + Pf1N1B4_1665 0.15 +1.5 1,908,938 + Pf1N1B4_1665 0.15 -0.5 1,908,939 - Pf1N1B4_1665 0.15 -0.1 1,908,939 - Pf1N1B4_1665 0.15 -1.9 1,908,939 - Pf1N1B4_1665 0.15 +0.2 1,908,939 - Pf1N1B4_1665 0.15 +0.1 1,909,028 + Pf1N1B4_1665 0.20 -0.5 1,909,028 + Pf1N1B4_1665 0.20 +1.0 1,909,028 + Pf1N1B4_1665 0.20 +0.9 1,909,029 - Pf1N1B4_1665 0.20 -0.9 1,909,100 + Pf1N1B4_1665 0.24 -0.5 1,909,101 - Pf1N1B4_1665 0.25 +1.5 1,909,491 + Pf1N1B4_1665 0.47 +0.0 1,909,491 + Pf1N1B4_1665 0.47 -0.2 1,909,492 - Pf1N1B4_1665 0.47 +0.5
Or see this region's nucleotide sequence