Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1635 and Pf1N1B4_1636 are separated by 37 nucleotides Pf1N1B4_1636 and Pf1N1B4_1637 are separated by 6 nucleotides Pf1N1B4_1637 and Pf1N1B4_1638 are separated by 84 nucleotides Pf1N1B4_1638 and Pf1N1B4_1639 are separated by 364 nucleotides
Pf1N1B4_1635 - Hydrolases of the alpha/beta superfamily, at 1,879,183 to 1,880,157
_1635
Pf1N1B4_1636 - FIG00954739: hypothetical protein, at 1,880,195 to 1,880,611
_1636
Pf1N1B4_1637 - FIG00954739: hypothetical protein, at 1,880,618 to 1,881,076
_1637
Pf1N1B4_1638 - hypothetical protein, at 1,881,161 to 1,881,487
_1638
Pf1N1B4_1639 - transport permease protein of gamma-aminobutyrate, at 1,881,852 to 1,883,246
_1639
Position (kb)
1880
1881
1882 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1879.663 kb on + strand, within Pf1N1B4_1635 at 1879.750 kb on + strand, within Pf1N1B4_1635 at 1879.750 kb on + strand, within Pf1N1B4_1635 at 1879.751 kb on - strand, within Pf1N1B4_1635 at 1879.751 kb on - strand, within Pf1N1B4_1635 at 1879.799 kb on + strand, within Pf1N1B4_1635 at 1879.800 kb on - strand, within Pf1N1B4_1635 at 1879.936 kb on + strand, within Pf1N1B4_1635 at 1879.972 kb on + strand, within Pf1N1B4_1635 at 1879.972 kb on + strand, within Pf1N1B4_1635 at 1879.986 kb on + strand, within Pf1N1B4_1635 at 1879.986 kb on + strand, within Pf1N1B4_1635 at 1879.986 kb on + strand, within Pf1N1B4_1635 at 1879.986 kb on + strand, within Pf1N1B4_1635 at 1879.986 kb on + strand, within Pf1N1B4_1635 at 1879.987 kb on - strand, within Pf1N1B4_1635 at 1879.987 kb on - strand, within Pf1N1B4_1635 at 1880.065 kb on + strand at 1880.066 kb on - strand at 1880.066 kb on - strand at 1880.079 kb on - strand at 1880.079 kb on - strand at 1880.079 kb on - strand at 1880.079 kb on - strand at 1880.180 kb on + strand at 1880.558 kb on + strand, within Pf1N1B4_1636 at 1880.559 kb on - strand, within Pf1N1B4_1636 at 1880.750 kb on + strand, within Pf1N1B4_1637 at 1880.750 kb on + strand, within Pf1N1B4_1637 at 1880.751 kb on - strand, within Pf1N1B4_1637 at 1880.751 kb on - strand, within Pf1N1B4_1637 at 1880.751 kb on - strand, within Pf1N1B4_1637 at 1881.141 kb on - strand at 1881.163 kb on - strand at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.216 kb on + strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.217 kb on - strand, within Pf1N1B4_1638 at 1881.252 kb on + strand, within Pf1N1B4_1638 at 1881.279 kb on + strand, within Pf1N1B4_1638 at 1881.279 kb on + strand, within Pf1N1B4_1638 at 1881.279 kb on + strand, within Pf1N1B4_1638 at 1881.279 kb on + strand, within Pf1N1B4_1638 at 1881.280 kb on - strand, within Pf1N1B4_1638 at 1881.291 kb on + strand, within Pf1N1B4_1638 at 1881.291 kb on + strand, within Pf1N1B4_1638 at 1881.291 kb on + strand, within Pf1N1B4_1638 at 1881.291 kb on + strand, within Pf1N1B4_1638 at 1881.292 kb on - strand, within Pf1N1B4_1638 at 1881.292 kb on - strand, within Pf1N1B4_1638 at 1881.292 kb on - strand, within Pf1N1B4_1638 at 1881.292 kb on - strand, within Pf1N1B4_1638 at 1881.292 kb on - strand, within Pf1N1B4_1638 at 1881.295 kb on + strand, within Pf1N1B4_1638 at 1881.295 kb on + strand, within Pf1N1B4_1638 at 1881.295 kb on + strand, within Pf1N1B4_1638 at 1881.295 kb on + strand, within Pf1N1B4_1638 at 1881.295 kb on + strand, within Pf1N1B4_1638 at 1881.296 kb on - strand, within Pf1N1B4_1638 at 1881.296 kb on - strand, within Pf1N1B4_1638 at 1881.296 kb on - strand, within Pf1N1B4_1638 at 1881.296 kb on - strand, within Pf1N1B4_1638 at 1881.296 kb on - strand, within Pf1N1B4_1638 at 1881.455 kb on - strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.478 kb on + strand at 1881.479 kb on - strand at 1881.479 kb on - strand at 1881.532 kb on + strand at 1881.533 kb on - strand at 1881.533 kb on - strand at 1881.567 kb on + strand at 1881.568 kb on - strand at 1881.568 kb on - strand at 1881.568 kb on - strand at 1881.578 kb on + strand at 1881.578 kb on + strand at 1881.644 kb on + strand at 1881.644 kb on + strand at 1881.737 kb on + strand at 1881.765 kb on - strand at 1881.765 kb on - strand at 1881.834 kb on + strand at 1881.834 kb on + strand at 1881.834 kb on + strand at 1881.835 kb on - strand at 1881.900 kb on + strand at 1881.901 kb on - strand at 1881.934 kb on - strand at 1882.008 kb on + strand, within Pf1N1B4_1639 at 1882.008 kb on + strand, within Pf1N1B4_1639 at 1882.008 kb on + strand, within Pf1N1B4_1639 at 1882.008 kb on + strand, within Pf1N1B4_1639 at 1882.009 kb on - strand, within Pf1N1B4_1639 at 1882.009 kb on - strand, within Pf1N1B4_1639 at 1882.009 kb on - strand, within Pf1N1B4_1639 at 1882.009 kb on - strand, within Pf1N1B4_1639 at 1882.009 kb on - strand, within Pf1N1B4_1639 at 1882.009 kb on - strand at 1882.009 kb on - strand at 1882.021 kb on + strand at 1882.022 kb on - strand at 1882.022 kb on - strand, within Pf1N1B4_1639 at 1882.076 kb on + strand, within Pf1N1B4_1639
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 1,879,663 + Pf1N1B4_1635 0.49 -0.1 1,879,750 + Pf1N1B4_1635 0.58 -0.9 1,879,750 + Pf1N1B4_1635 0.58 -1.9 1,879,751 - Pf1N1B4_1635 0.58 -0.8 1,879,751 - Pf1N1B4_1635 0.58 -0.3 1,879,799 + Pf1N1B4_1635 0.63 -1.4 1,879,800 - Pf1N1B4_1635 0.63 -0.6 1,879,936 + Pf1N1B4_1635 0.77 +0.8 1,879,972 + Pf1N1B4_1635 0.81 +0.7 1,879,972 + Pf1N1B4_1635 0.81 +1.7 1,879,986 + Pf1N1B4_1635 0.82 +0.9 1,879,986 + Pf1N1B4_1635 0.82 +2.0 1,879,986 + Pf1N1B4_1635 0.82 -1.2 1,879,986 + Pf1N1B4_1635 0.82 -0.6 1,879,986 + Pf1N1B4_1635 0.82 +0.1 1,879,987 - Pf1N1B4_1635 0.82 -0.4 1,879,987 - Pf1N1B4_1635 0.82 +0.6 1,880,065 + +0.4 1,880,066 - +0.4 1,880,066 - -0.3 1,880,079 - -0.1 1,880,079 - +0.6 1,880,079 - +0.4 1,880,079 - +0.2 1,880,180 + +1.1 1,880,558 + Pf1N1B4_1636 0.87 -0.0 1,880,559 - Pf1N1B4_1636 0.87 -1.1 1,880,750 + Pf1N1B4_1637 0.29 -0.6 1,880,750 + Pf1N1B4_1637 0.29 -1.4 1,880,751 - Pf1N1B4_1637 0.29 +0.1 1,880,751 - Pf1N1B4_1637 0.29 -2.0 1,880,751 - Pf1N1B4_1637 0.29 -0.9 1,881,141 - -0.4 1,881,163 - +0.9 1,881,216 + Pf1N1B4_1638 0.17 -1.1 1,881,216 + Pf1N1B4_1638 0.17 +2.3 1,881,216 + Pf1N1B4_1638 0.17 +0.4 1,881,216 + Pf1N1B4_1638 0.17 +1.0 1,881,216 + Pf1N1B4_1638 0.17 +0.1 1,881,216 + Pf1N1B4_1638 0.17 +1.1 1,881,217 - Pf1N1B4_1638 0.17 +0.1 1,881,217 - Pf1N1B4_1638 0.17 -0.1 1,881,217 - Pf1N1B4_1638 0.17 +0.4 1,881,217 - Pf1N1B4_1638 0.17 -1.7 1,881,217 - Pf1N1B4_1638 0.17 -0.4 1,881,217 - Pf1N1B4_1638 0.17 +1.0 1,881,217 - Pf1N1B4_1638 0.17 -0.1 1,881,217 - Pf1N1B4_1638 0.17 +0.4 1,881,217 - Pf1N1B4_1638 0.17 +0.8 1,881,217 - Pf1N1B4_1638 0.17 -0.4 1,881,217 - Pf1N1B4_1638 0.17 +0.4 1,881,252 + Pf1N1B4_1638 0.28 +0.7 1,881,279 + Pf1N1B4_1638 0.36 -2.1 1,881,279 + Pf1N1B4_1638 0.36 +0.9 1,881,279 + Pf1N1B4_1638 0.36 -0.9 1,881,279 + Pf1N1B4_1638 0.36 +1.0 1,881,280 - Pf1N1B4_1638 0.36 -1.4 1,881,291 + Pf1N1B4_1638 0.40 -0.4 1,881,291 + Pf1N1B4_1638 0.40 -0.1 1,881,291 + Pf1N1B4_1638 0.40 -0.7 1,881,291 + Pf1N1B4_1638 0.40 +0.4 1,881,292 - Pf1N1B4_1638 0.40 -0.1 1,881,292 - Pf1N1B4_1638 0.40 +2.2 1,881,292 - Pf1N1B4_1638 0.40 -0.5 1,881,292 - Pf1N1B4_1638 0.40 -1.6 1,881,292 - Pf1N1B4_1638 0.40 -1.1 1,881,295 + Pf1N1B4_1638 0.41 +1.4 1,881,295 + Pf1N1B4_1638 0.41 +1.2 1,881,295 + Pf1N1B4_1638 0.41 -1.2 1,881,295 + Pf1N1B4_1638 0.41 +0.9 1,881,295 + Pf1N1B4_1638 0.41 -0.9 1,881,296 - Pf1N1B4_1638 0.41 -0.9 1,881,296 - Pf1N1B4_1638 0.41 -1.5 1,881,296 - Pf1N1B4_1638 0.41 -1.4 1,881,296 - Pf1N1B4_1638 0.41 +1.3 1,881,296 - Pf1N1B4_1638 0.41 +1.2 1,881,455 - -1.1 1,881,478 + -1.1 1,881,478 + +0.4 1,881,478 + -0.9 1,881,478 + -1.7 1,881,478 + +0.9 1,881,478 + -0.5 1,881,478 + +1.4 1,881,478 + +1.1 1,881,479 - +0.4 1,881,479 - -1.7 1,881,532 + -0.4 1,881,533 - +0.4 1,881,533 - -1.9 1,881,567 + -0.1 1,881,568 - +1.4 1,881,568 - -1.9 1,881,568 - +1.4 1,881,578 + +1.6 1,881,578 + +0.1 1,881,644 + -0.4 1,881,644 + -0.1 1,881,737 + +1.7 1,881,765 - +0.4 1,881,765 - -1.9 1,881,834 + +0.7 1,881,834 + -0.1 1,881,834 + +1.4 1,881,835 - +1.0 1,881,900 + -0.1 1,881,901 - +0.3 1,881,934 - +1.4 1,882,008 + Pf1N1B4_1639 0.11 +0.1 1,882,008 + Pf1N1B4_1639 0.11 +0.2 1,882,008 + Pf1N1B4_1639 0.11 +1.0 1,882,008 + Pf1N1B4_1639 0.11 -0.7 1,882,009 - Pf1N1B4_1639 0.11 +1.7 1,882,009 - Pf1N1B4_1639 0.11 +1.0 1,882,009 - Pf1N1B4_1639 0.11 -0.9 1,882,009 - Pf1N1B4_1639 0.11 -0.0 1,882,009 - Pf1N1B4_1639 0.11 +0.8 1,882,009 - -0.1 1,882,009 - +1.4 1,882,021 + +0.4 1,882,022 - +1.4 1,882,022 - Pf1N1B4_1639 0.12 -0.1 1,882,076 + Pf1N1B4_1639 0.16 -0.1
Or see this region's nucleotide sequence