Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1329 and Pf1N1B4_1330 are separated by 145 nucleotides Pf1N1B4_1330 and Pf1N1B4_1331 are separated by 242 nucleotides
Pf1N1B4_1329 - Cobalamin biosynthesis protein CobG, at 1,523,778 to 1,525,079
_1329
Pf1N1B4_1330 - hypothetical protein, at 1,525,225 to 1,525,881
_1330
Pf1N1B4_1331 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 1,526,124 to 1,527,335
_1331
Position (kb)
1525
1526 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1524.283 kb on - strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.295 kb on + strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.296 kb on - strand, within Pf1N1B4_1329 at 1524.301 kb on + strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.302 kb on - strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.758 kb on + strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1524.759 kb on - strand, within Pf1N1B4_1329 at 1525.065 kb on + strand at 1525.066 kb on - strand at 1525.145 kb on - strand at 1525.201 kb on - strand at 1525.201 kb on - strand at 1525.208 kb on - strand at 1525.236 kb on - strand at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.377 kb on - strand, within Pf1N1B4_1330 at 1525.383 kb on + strand, within Pf1N1B4_1330 at 1525.384 kb on - strand, within Pf1N1B4_1330 at 1525.448 kb on + strand, within Pf1N1B4_1330 at 1525.464 kb on + strand, within Pf1N1B4_1330 at 1525.729 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.731 kb on - strand, within Pf1N1B4_1330 at 1525.759 kb on + strand, within Pf1N1B4_1330 at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.825 kb on - strand at 1525.882 kb on - strand at 1525.975 kb on + strand at 1526.016 kb on + strand at 1526.016 kb on + strand at 1526.339 kb on - strand, within Pf1N1B4_1331 at 1526.482 kb on + strand, within Pf1N1B4_1331 at 1526.483 kb on - strand, within Pf1N1B4_1331
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 1,524,283 - Pf1N1B4_1329 0.39 -0.7 1,524,295 + Pf1N1B4_1329 0.40 +1.3 1,524,295 + Pf1N1B4_1329 0.40 -0.7 1,524,295 + +0.3 1,524,295 + Pf1N1B4_1329 0.40 -0.6 1,524,295 + Pf1N1B4_1329 0.40 +0.3 1,524,295 + Pf1N1B4_1329 0.40 +0.3 1,524,295 + Pf1N1B4_1329 0.40 -0.7 1,524,296 - Pf1N1B4_1329 0.40 -0.8 1,524,296 - Pf1N1B4_1329 0.40 -1.8 1,524,296 - Pf1N1B4_1329 0.40 -1.0 1,524,296 - Pf1N1B4_1329 0.40 -1.4 1,524,296 - Pf1N1B4_1329 0.40 -1.3 1,524,296 - Pf1N1B4_1329 0.40 +1.3 1,524,296 - Pf1N1B4_1329 0.40 -0.3 1,524,296 - Pf1N1B4_1329 0.40 -1.1 1,524,296 - Pf1N1B4_1329 0.40 -0.9 1,524,296 - Pf1N1B4_1329 0.40 +1.1 1,524,296 - Pf1N1B4_1329 0.40 -0.3 1,524,296 - Pf1N1B4_1329 0.40 -0.3 1,524,296 - Pf1N1B4_1329 0.40 +0.9 1,524,301 + Pf1N1B4_1329 0.40 +0.8 1,524,302 - Pf1N1B4_1329 0.40 -1.3 1,524,302 - Pf1N1B4_1329 0.40 -1.3 1,524,302 - Pf1N1B4_1329 0.40 +0.2 1,524,302 - Pf1N1B4_1329 0.40 +0.3 1,524,302 - Pf1N1B4_1329 0.40 +0.2 1,524,758 + Pf1N1B4_1329 0.75 -1.4 1,524,758 + Pf1N1B4_1329 0.75 -0.7 1,524,759 - Pf1N1B4_1329 0.75 -0.1 1,524,759 - Pf1N1B4_1329 0.75 -1.7 1,524,759 - Pf1N1B4_1329 0.75 -1.0 1,524,759 - Pf1N1B4_1329 0.75 +2.1 1,525,065 + +0.5 1,525,066 - +1.7 1,525,145 - +0.8 1,525,201 - +0.5 1,525,201 - +1.3 1,525,208 - -0.5 1,525,236 - +0.2 1,525,377 - Pf1N1B4_1330 0.23 -0.3 1,525,377 - Pf1N1B4_1330 0.23 -0.6 1,525,377 - Pf1N1B4_1330 0.23 -2.1 1,525,383 + Pf1N1B4_1330 0.24 -1.0 1,525,384 - Pf1N1B4_1330 0.24 +1.3 1,525,448 + Pf1N1B4_1330 0.34 -1.7 1,525,464 + Pf1N1B4_1330 0.36 +0.3 1,525,729 - Pf1N1B4_1330 0.77 -1.3 1,525,731 - Pf1N1B4_1330 0.77 -2.1 1,525,731 - Pf1N1B4_1330 0.77 +0.9 1,525,759 + Pf1N1B4_1330 0.81 -1.3 1,525,825 - +0.2 1,525,825 - -0.9 1,525,825 - +2.3 1,525,882 - +1.3 1,525,975 + -0.7 1,526,016 + -0.8 1,526,016 + +0.6 1,526,339 - Pf1N1B4_1331 0.18 -1.5 1,526,482 + Pf1N1B4_1331 0.30 +0.1 1,526,483 - Pf1N1B4_1331 0.30 -2.2
Or see this region's nucleotide sequence