Experiment: Choline Lysine 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1050 and Pf1N1B4_1051 are separated by 226 nucleotides Pf1N1B4_1051 and Pf1N1B4_1052 are separated by 179 nucleotides Pf1N1B4_1052 and Pf1N1B4_1053 are separated by 40 nucleotides Pf1N1B4_1053 and Pf1N1B4_1054 are separated by 15 nucleotides
Pf1N1B4_1050 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 1,186,216 to 1,187,319
_1050
Pf1N1B4_1051 - Glutathione peroxidase family protein, at 1,187,546 to 1,187,974
_1051
Pf1N1B4_1052 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 1,188,154 to 1,188,639
_1052
Pf1N1B4_1053 - hypothetical protein, at 1,188,680 to 1,188,991
_1053
Pf1N1B4_1054 - Phosphate acetyltransferase (EC 2.3.1.8), at 1,189,007 to 1,189,840
_1054
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1187.189 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.192 kb on + strand, within Pf1N1B4_1050 at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.338 kb on + strand at 1187.339 kb on - strand at 1187.345 kb on + strand at 1187.346 kb on - strand at 1187.359 kb on + strand at 1187.389 kb on - strand at 1187.389 kb on - strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.396 kb on + strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.397 kb on - strand at 1187.431 kb on - strand at 1187.475 kb on + strand at 1187.475 kb on + strand at 1187.476 kb on - strand at 1187.501 kb on + strand at 1187.599 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.709 kb on + strand, within Pf1N1B4_1051 at 1187.710 kb on - strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.772 kb on + strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.773 kb on - strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand, within Pf1N1B4_1051 at 1187.874 kb on + strand at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.875 kb on - strand, within Pf1N1B4_1051 at 1187.904 kb on + strand, within Pf1N1B4_1051 at 1187.905 kb on - strand, within Pf1N1B4_1051 at 1187.978 kb on + strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1187.979 kb on - strand at 1188.100 kb on - strand at 1188.428 kb on + strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.429 kb on - strand, within Pf1N1B4_1052 at 1188.454 kb on + strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.455 kb on - strand, within Pf1N1B4_1052 at 1188.522 kb on + strand, within Pf1N1B4_1052 at 1188.539 kb on + strand, within Pf1N1B4_1052 at 1188.601 kb on - strand at 1188.601 kb on - strand at 1188.602 kb on + strand at 1188.603 kb on - strand at 1188.603 kb on - strand at 1188.621 kb on + strand at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.871 kb on + strand, within Pf1N1B4_1053 at 1188.968 kb on + strand at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.283 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054 at 1189.352 kb on + strand, within Pf1N1B4_1054
Per-strain Table
Position Strand Gene LocusTag Fraction Choline Lysine 6 mM remove 1,187,189 + Pf1N1B4_1050 0.88 +1.8 1,187,192 + Pf1N1B4_1050 0.88 -0.4 1,187,192 + Pf1N1B4_1050 0.88 -0.4 1,187,338 + +1.0 1,187,338 + -1.4 1,187,338 + -1.4 1,187,339 - +0.5 1,187,345 + +2.8 1,187,346 - +0.8 1,187,359 + +0.2 1,187,389 - +0.2 1,187,389 - +0.6 1,187,396 + -1.8 1,187,396 + +0.6 1,187,396 + -1.4 1,187,396 + -0.4 1,187,396 + +0.2 1,187,396 + -0.4 1,187,397 - +1.8 1,187,397 - -0.4 1,187,397 - -1.8 1,187,431 - -0.8 1,187,475 + -0.1 1,187,475 + -0.8 1,187,476 - +3.8 1,187,501 + -1.1 1,187,599 + Pf1N1B4_1051 0.12 -0.6 1,187,709 + Pf1N1B4_1051 0.38 -0.4 1,187,709 + Pf1N1B4_1051 0.38 +0.2 1,187,709 + Pf1N1B4_1051 0.38 +0.5 1,187,710 - Pf1N1B4_1051 0.38 -0.4 1,187,772 + Pf1N1B4_1051 0.53 -0.4 1,187,772 + Pf1N1B4_1051 0.53 +1.2 1,187,773 - Pf1N1B4_1051 0.53 -0.3 1,187,773 - Pf1N1B4_1051 0.53 +0.2 1,187,773 - Pf1N1B4_1051 0.53 -0.8 1,187,874 + Pf1N1B4_1051 0.76 +0.6 1,187,874 + Pf1N1B4_1051 0.76 +0.3 1,187,874 + Pf1N1B4_1051 0.76 -0.6 1,187,874 + Pf1N1B4_1051 0.76 +0.6 1,187,874 + -1.1 1,187,875 - Pf1N1B4_1051 0.77 +1.2 1,187,875 - Pf1N1B4_1051 0.77 +1.0 1,187,875 - Pf1N1B4_1051 0.77 +0.5 1,187,875 - Pf1N1B4_1051 0.77 +0.5 1,187,875 - Pf1N1B4_1051 0.77 -1.0 1,187,875 - Pf1N1B4_1051 0.77 +0.2 1,187,875 - Pf1N1B4_1051 0.77 +0.2 1,187,904 + Pf1N1B4_1051 0.83 +0.1 1,187,905 - Pf1N1B4_1051 0.84 -0.4 1,187,978 + +1.0 1,187,979 - -0.5 1,187,979 - +1.5 1,187,979 - +0.2 1,187,979 - -2.7 1,187,979 - +0.1 1,188,100 - -2.1 1,188,428 + Pf1N1B4_1052 0.56 +1.2 1,188,429 - Pf1N1B4_1052 0.57 -1.2 1,188,429 - Pf1N1B4_1052 0.57 -0.4 1,188,454 + Pf1N1B4_1052 0.62 -0.8 1,188,455 - Pf1N1B4_1052 0.62 +1.6 1,188,455 - Pf1N1B4_1052 0.62 -0.4 1,188,522 + Pf1N1B4_1052 0.76 +0.1 1,188,539 + Pf1N1B4_1052 0.79 +0.4 1,188,601 - -1.8 1,188,601 - +2.0 1,188,602 + -0.4 1,188,603 - -0.0 1,188,603 - +2.2 1,188,621 + +0.2 1,188,871 + Pf1N1B4_1053 0.61 +1.6 1,188,871 + Pf1N1B4_1053 0.61 +0.2 1,188,968 + +0.4 1,189,283 + Pf1N1B4_1054 0.33 -1.1 1,189,283 + Pf1N1B4_1054 0.33 -0.5 1,189,352 + Pf1N1B4_1054 0.41 +1.8 1,189,352 + Pf1N1B4_1054 0.41 +0.9
Or see this region's nucleotide sequence