Experiment: PYE with Cisplatin 0.0025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02961 and CCNA_03983 are separated by 123 nucleotides CCNA_03983 and CCNA_02962 overlap by 11 nucleotides CCNA_02962 and CCNA_02963 are separated by 64 nucleotides CCNA_02963 and CCNA_03984 are separated by 24 nucleotides CCNA_03984 and CCNA_02964 are separated by 60 nucleotides
CCNA_02961: CCNA_02961 - NeuB-family N-acetylneuraminate synthase, at 3,117,581 to 3,118,651
_02961
CCNA_03983: CCNA_03983 - HicA-related toxin-antitoxin protein, at 3,118,775 to 3,119,035
_03983
CCNA_02962: CCNA_02962 - HicB-family RNAse H fold protein, at 3,119,025 to 3,119,252
_02962
CCNA_02963: CCNA_02963 - hypothetical protein, at 3,119,317 to 3,119,577
_02963
CCNA_03984: CCNA_03984 - hypothetical protein, at 3,119,602 to 3,119,790
_03984
CCNA_02964: CCNA_02964 - thioredoxin-disulfide reductase, at 3,119,851 to 3,120,858
_02964
Position (kb)
3119
3120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3118.352 kb on + strand, within CCNA_02961 at 3118.362 kb on + strand, within CCNA_02961 at 3118.370 kb on - strand, within CCNA_02961 at 3118.372 kb on + strand, within CCNA_02961 at 3118.382 kb on - strand, within CCNA_02961 at 3118.390 kb on + strand, within CCNA_02961 at 3118.454 kb on + strand, within CCNA_02961 at 3118.455 kb on - strand, within CCNA_02961 at 3118.455 kb on - strand, within CCNA_02961 at 3118.464 kb on + strand, within CCNA_02961 at 3118.469 kb on + strand, within CCNA_02961 at 3118.472 kb on - strand, within CCNA_02961 at 3118.477 kb on - strand, within CCNA_02961 at 3118.523 kb on - strand, within CCNA_02961 at 3118.537 kb on - strand, within CCNA_02961 at 3118.550 kb on + strand at 3118.550 kb on - strand at 3118.630 kb on + strand at 3118.642 kb on + strand at 3118.648 kb on + strand at 3118.650 kb on - strand at 3118.671 kb on - strand at 3118.671 kb on - strand at 3118.777 kb on + strand at 3118.868 kb on + strand, within CCNA_03983 at 3118.875 kb on + strand, within CCNA_03983 at 3118.995 kb on - strand, within CCNA_03983 at 3119.245 kb on + strand at 3119.245 kb on + strand at 3119.253 kb on - strand at 3119.264 kb on - strand at 3119.301 kb on - strand at 3119.313 kb on + strand at 3119.366 kb on + strand, within CCNA_02963 at 3119.473 kb on + strand, within CCNA_02963 at 3119.484 kb on + strand, within CCNA_02963 at 3119.484 kb on + strand, within CCNA_02963 at 3119.492 kb on - strand, within CCNA_02963 at 3119.550 kb on - strand, within CCNA_02963 at 3119.585 kb on + strand at 3119.636 kb on + strand, within CCNA_03984 at 3119.644 kb on - strand, within CCNA_03984 at 3119.655 kb on + strand, within CCNA_03984 at 3119.707 kb on + strand, within CCNA_03984 at 3119.742 kb on - strand, within CCNA_03984 at 3119.756 kb on + strand, within CCNA_03984 at 3119.767 kb on + strand, within CCNA_03984 at 3119.778 kb on - strand at 3119.780 kb on - strand at 3119.793 kb on + strand at 3119.830 kb on + strand at 3119.837 kb on + strand at 3119.843 kb on - strand at 3119.845 kb on - strand at 3119.852 kb on + strand at 3119.852 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Cisplatin 0.0025 mg/ml remove 3,118,352 + CCNA_02961 0.72 +0.5 3,118,362 + CCNA_02961 0.73 +3.3 3,118,370 - CCNA_02961 0.74 +0.8 3,118,372 + CCNA_02961 0.74 -1.0 3,118,382 - CCNA_02961 0.75 +0.6 3,118,390 + CCNA_02961 0.76 -0.2 3,118,454 + CCNA_02961 0.82 +0.9 3,118,455 - CCNA_02961 0.82 +1.0 3,118,455 - CCNA_02961 0.82 +0.7 3,118,464 + CCNA_02961 0.82 -0.2 3,118,469 + CCNA_02961 0.83 +0.9 3,118,472 - CCNA_02961 0.83 +0.1 3,118,477 - CCNA_02961 0.84 +0.2 3,118,523 - CCNA_02961 0.88 -0.5 3,118,537 - CCNA_02961 0.89 +1.2 3,118,550 + -1.1 3,118,550 - +0.3 3,118,630 + -1.4 3,118,642 + +2.3 3,118,648 + -0.9 3,118,650 - +0.3 3,118,671 - -1.0 3,118,671 - +0.3 3,118,777 + -1.0 3,118,868 + CCNA_03983 0.36 -1.0 3,118,875 + CCNA_03983 0.38 +0.1 3,118,995 - CCNA_03983 0.84 -2.8 3,119,245 + +0.0 3,119,245 + +0.4 3,119,253 - +0.2 3,119,264 - -0.8 3,119,301 - +0.9 3,119,313 + -2.1 3,119,366 + CCNA_02963 0.19 -1.3 3,119,473 + CCNA_02963 0.60 -1.8 3,119,484 + CCNA_02963 0.64 -0.9 3,119,484 + CCNA_02963 0.64 -0.9 3,119,492 - CCNA_02963 0.67 -0.8 3,119,550 - CCNA_02963 0.89 +0.1 3,119,585 + -1.4 3,119,636 + CCNA_03984 0.18 -1.6 3,119,644 - CCNA_03984 0.22 -1.4 3,119,655 + CCNA_03984 0.28 +1.0 3,119,707 + CCNA_03984 0.56 +0.1 3,119,742 - CCNA_03984 0.74 -0.2 3,119,756 + CCNA_03984 0.81 +0.2 3,119,767 + CCNA_03984 0.87 -1.6 3,119,778 - -0.4 3,119,780 - +0.0 3,119,793 + -0.8 3,119,830 + -0.8 3,119,837 + -1.4 3,119,843 - +0.2 3,119,845 - -1.0 3,119,852 + -1.5 3,119,852 + -1.0
Or see this region's nucleotide sequence