Strain Fitness in Ralstonia sp. UNC404CL21Col around ABZR87_RS22215

Experiment: soil sample 9; outgrowth in LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntABZR87_RS22205 and ABZR87_RS22210 overlap by 4 nucleotidesABZR87_RS22210 and ABZR87_RS22215 are separated by 19 nucleotidesABZR87_RS22215 and ABZR87_RS22220 are separated by 33 nucleotidesABZR87_RS22220 and ABZR87_RS22225 are separated by 85 nucleotides ABZR87_RS22205: ABZR87_RS22205 - flagellar biosynthetic protein FliO, at 1,345,858 to 1,346,259 _RS22205 ABZR87_RS22210: ABZR87_RS22210 - flagellar type III secretion system pore protein FliP, at 1,346,256 to 1,347,005 _RS22210 ABZR87_RS22215: ABZR87_RS22215 - flagellar biosynthesis protein FliQ, at 1,347,025 to 1,347,294 _RS22215 ABZR87_RS22220: ABZR87_RS22220 - flagellar biosynthetic protein FliR, at 1,347,328 to 1,348,122 _RS22220 ABZR87_RS22225: ABZR87_RS22225 - 3-ketoacyl-ACP reductase FabG2, at 1,348,208 to 1,348,936 _RS22225 Position (kb) 1347 1348Strain fitness (log2 ratio) -1 0 1 2 3at 1346.267 kb on + strandat 1346.267 kb on + strandat 1346.267 kb on + strandat 1346.267 kb on + strandat 1346.267 kb on + strandat 1346.267 kb on + strandat 1346.268 kb on - strandat 1346.268 kb on - strandat 1346.268 kb on - strandat 1346.268 kb on - strandat 1346.379 kb on + strand, within ABZR87_RS22210at 1346.379 kb on + strand, within ABZR87_RS22210at 1346.379 kb on + strand, within ABZR87_RS22210at 1346.380 kb on - strand, within ABZR87_RS22210at 1346.380 kb on - strand, within ABZR87_RS22210at 1346.592 kb on + strand, within ABZR87_RS22210at 1346.592 kb on + strand, within ABZR87_RS22210at 1346.592 kb on + strand, within ABZR87_RS22210at 1346.593 kb on - strand, within ABZR87_RS22210at 1346.604 kb on + strand, within ABZR87_RS22210at 1346.604 kb on + strand, within ABZR87_RS22210at 1346.604 kb on + strand, within ABZR87_RS22210at 1346.605 kb on - strand, within ABZR87_RS22210at 1347.037 kb on + strandat 1347.038 kb on - strandat 1347.283 kb on + strandat 1347.283 kb on + strandat 1347.284 kb on - strandat 1347.284 kb on - strandat 1347.284 kb on - strandat 1347.373 kb on + strandat 1347.373 kb on + strandat 1347.373 kb on + strandat 1347.373 kb on + strandat 1347.373 kb on + strandat 1347.373 kb on + strandat 1347.374 kb on - strandat 1347.374 kb on - strandat 1347.453 kb on + strand, within ABZR87_RS22220at 1347.453 kb on + strand, within ABZR87_RS22220at 1347.453 kb on + strand, within ABZR87_RS22220at 1347.453 kb on + strand, within ABZR87_RS22220at 1347.454 kb on - strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.532 kb on + strand, within ABZR87_RS22220at 1347.533 kb on - strand, within ABZR87_RS22220at 1347.533 kb on - strand, within ABZR87_RS22220at 1347.533 kb on - strand, within ABZR87_RS22220at 1347.533 kb on - strand, within ABZR87_RS22220at 1348.172 kb on + strandat 1348.173 kb on - strandat 1348.265 kb on + strandat 1348.265 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction soil sample 9; outgrowth in LB
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1,346,267 + +2.2
1,346,267 + +0.9
1,346,267 + +2.3
1,346,267 + +2.2
1,346,267 + +1.6
1,346,267 + +1.6
1,346,268 - +1.9
1,346,268 - +3.0
1,346,268 - +2.6
1,346,268 - +2.4
1,346,379 + ABZR87_RS22210 0.16 +2.8
1,346,379 + ABZR87_RS22210 0.16 +2.0
1,346,379 + ABZR87_RS22210 0.16 +2.5
1,346,380 - ABZR87_RS22210 0.17 +2.3
1,346,380 - ABZR87_RS22210 0.17 +2.3
1,346,592 + ABZR87_RS22210 0.45 +2.8
1,346,592 + ABZR87_RS22210 0.45 +3.0
1,346,592 + ABZR87_RS22210 0.45 +2.5
1,346,593 - ABZR87_RS22210 0.45 +2.6
1,346,604 + ABZR87_RS22210 0.46 +2.6
1,346,604 + ABZR87_RS22210 0.46 +2.6
1,346,604 + ABZR87_RS22210 0.46 +2.6
1,346,605 - ABZR87_RS22210 0.47 +2.3
1,347,037 + +2.3
1,347,038 - +0.2
1,347,283 + +0.5
1,347,283 + +1.0
1,347,284 - +1.7
1,347,284 - +2.2
1,347,284 - +1.9
1,347,373 + +2.2
1,347,373 + +2.5
1,347,373 + +2.8
1,347,373 + +2.4
1,347,373 + +2.7
1,347,373 + +2.9
1,347,374 - +2.9
1,347,374 - +2.3
1,347,453 + ABZR87_RS22220 0.16 +2.5
1,347,453 + ABZR87_RS22220 0.16 +2.9
1,347,453 + ABZR87_RS22220 0.16 +1.9
1,347,453 + ABZR87_RS22220 0.16 +2.7
1,347,454 - ABZR87_RS22220 0.16 +3.0
1,347,532 + ABZR87_RS22220 0.26 +2.4
1,347,532 + ABZR87_RS22220 0.26 +2.5
1,347,532 + ABZR87_RS22220 0.26 +2.4
1,347,532 + ABZR87_RS22220 0.26 +2.4
1,347,532 + ABZR87_RS22220 0.26 +2.2
1,347,532 + ABZR87_RS22220 0.26 +2.5
1,347,533 - ABZR87_RS22220 0.26 +1.9
1,347,533 - ABZR87_RS22220 0.26 +2.7
1,347,533 - ABZR87_RS22220 0.26 +2.8
1,347,533 - ABZR87_RS22220 0.26 +2.8
1,348,172 + +1.0
1,348,173 - -0.3
1,348,265 + -0.2
1,348,265 + -1.2

Or see this region's nucleotide sequence