Experiment: nitrite 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livM and natD are separated by 3 nucleotides natD and livJ overlap by 1 nucleotides livJ and ndk are separated by 29 nucleotides ndk and Synpcc7942_2498 are separated by 99 nucleotides
Synpcc7942_2494: livM - putative branched-chain amino acid ABC transporter, permease protein, at 2,574,819 to 2,575,742
livM
Synpcc7942_2495: natD - integral membrane protein of the ABC-type Nat permease for neutral amino acids NatD, at 2,575,746 to 2,576,687
natD
Synpcc7942_2496: livJ - branched-chain amino-acid ABC transport system periplasmic binding protein, at 2,576,687 to 2,577,886
livJ
Synpcc7942_2497: ndk - nucleoside diphosphate kinase, at 2,577,916 to 2,578,371
ndk
Synpcc7942_2498: Synpcc7942_2498 - Band 7 protein, at 2,578,471 to 2,579,715
_2498
Position (kb)
2576
2577
2578 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2575.692 kb on - strand at 2575.716 kb on + strand at 2575.724 kb on - strand at 2575.732 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.749 kb on + strand at 2575.751 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.767 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.786 kb on - strand at 2575.787 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.800 kb on - strand at 2575.804 kb on + strand at 2575.812 kb on - strand at 2575.821 kb on + strand at 2575.822 kb on - strand at 2575.846 kb on - strand, within natD at 2575.884 kb on - strand, within natD at 2575.897 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.932 kb on - strand, within natD at 2575.937 kb on - strand, within natD at 2575.946 kb on + strand, within natD at 2575.991 kb on + strand, within natD at 2576.000 kb on + strand, within natD at 2576.092 kb on - strand, within natD at 2576.113 kb on + strand, within natD at 2576.129 kb on - strand, within natD at 2576.148 kb on - strand, within natD at 2576.149 kb on + strand, within natD at 2576.161 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.222 kb on + strand, within natD at 2576.230 kb on - strand, within natD at 2576.243 kb on - strand, within natD at 2576.249 kb on - strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.256 kb on - strand at 2576.258 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.263 kb on + strand, within natD at 2576.270 kb on - strand at 2576.271 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.284 kb on + strand, within natD at 2576.357 kb on + strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.392 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.464 kb on - strand, within natD at 2576.476 kb on + strand, within natD at 2576.479 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.486 kb on + strand, within natD at 2576.536 kb on + strand, within natD at 2576.544 kb on - strand, within natD at 2576.592 kb on + strand, within natD at 2576.613 kb on + strand at 2576.615 kb on - strand at 2576.621 kb on - strand at 2576.622 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.657 kb on + strand at 2576.657 kb on + strand at 2576.667 kb on - strand at 2576.698 kb on + strand at 2576.698 kb on + strand at 2576.707 kb on + strand at 2576.747 kb on + strand at 2576.750 kb on - strand at 2576.760 kb on - strand at 2576.775 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.776 kb on + strand at 2576.784 kb on - strand at 2576.792 kb on + strand at 2576.800 kb on - strand at 2576.800 kb on - strand at 2576.803 kb on + strand at 2576.803 kb on + strand at 2576.821 kb on + strand, within livJ at 2576.831 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.838 kb on + strand, within livJ at 2576.839 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.846 kb on - strand, within livJ at 2576.864 kb on + strand, within livJ at 2576.864 kb on + strand, within livJ at 2576.865 kb on - strand, within livJ at 2576.866 kb on - strand, within livJ at 2576.900 kb on - strand, within livJ at 2576.912 kb on + strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.920 kb on - strand, within livJ at 2576.959 kb on - strand, within livJ at 2576.973 kb on + strand, within livJ at 2576.998 kb on - strand, within livJ at 2577.021 kb on + strand, within livJ at 2577.048 kb on + strand, within livJ at 2577.066 kb on - strand, within livJ at 2577.091 kb on + strand, within livJ at 2577.116 kb on + strand, within livJ at 2577.116 kb on + strand, within livJ at 2577.165 kb on + strand, within livJ at 2577.166 kb on + strand, within livJ at 2577.193 kb on - strand, within livJ at 2577.213 kb on - strand, within livJ at 2577.246 kb on + strand, within livJ at 2577.254 kb on - strand, within livJ at 2577.260 kb on - strand, within livJ at 2577.277 kb on - strand, within livJ at 2577.277 kb on - strand, within livJ at 2577.356 kb on - strand, within livJ at 2577.376 kb on + strand, within livJ at 2577.387 kb on + strand, within livJ at 2577.395 kb on + strand, within livJ at 2577.401 kb on + strand, within livJ at 2577.403 kb on - strand, within livJ at 2577.423 kb on + strand, within livJ at 2577.424 kb on - strand, within livJ at 2577.482 kb on + strand, within livJ at 2577.611 kb on + strand, within livJ at 2577.619 kb on - strand, within livJ at 2577.619 kb on - strand, within livJ at 2577.632 kb on - strand, within livJ at 2577.668 kb on - strand, within livJ at 2577.670 kb on + strand, within livJ at 2577.688 kb on - strand, within livJ at 2577.701 kb on + strand, within livJ at 2577.717 kb on - strand, within livJ at 2577.722 kb on - strand, within livJ at 2577.755 kb on + strand, within livJ at 2577.763 kb on - strand, within livJ at 2577.763 kb on - strand, within livJ at 2577.769 kb on - strand at 2577.791 kb on + strand at 2577.829 kb on - strand at 2577.834 kb on - strand at 2577.835 kb on + strand at 2577.835 kb on + strand at 2577.843 kb on - strand at 2577.848 kb on + strand at 2577.856 kb on - strand at 2577.858 kb on + strand at 2577.889 kb on - strand at 2577.907 kb on - strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.369 kb on + strand at 2578.381 kb on + strand at 2578.382 kb on - strand at 2578.397 kb on + strand at 2578.405 kb on - strand at 2578.419 kb on - strand at 2578.438 kb on + strand at 2578.449 kb on + strand at 2578.467 kb on - strand at 2578.468 kb on + strand at 2578.468 kb on + strand at 2578.477 kb on - strand at 2578.477 kb on - strand at 2578.477 kb on - strand at 2578.495 kb on + strand at 2578.579 kb on + strand at 2578.579 kb on + strand at 2578.587 kb on - strand at 2578.601 kb on + strand, within Synpcc7942_2498 at 2578.621 kb on + strand, within Synpcc7942_2498 at 2578.631 kb on + strand, within Synpcc7942_2498 at 2578.631 kb on + strand, within Synpcc7942_2498 at 2578.639 kb on - strand, within Synpcc7942_2498 at 2578.682 kb on - strand, within Synpcc7942_2498 at 2578.712 kb on + strand, within Synpcc7942_2498 at 2578.777 kb on + strand, within Synpcc7942_2498 at 2578.801 kb on + strand, within Synpcc7942_2498 at 2578.811 kb on + strand, within Synpcc7942_2498 at 2578.815 kb on + strand, within Synpcc7942_2498 at 2578.833 kb on - strand, within Synpcc7942_2498 at 2578.834 kb on + strand, within Synpcc7942_2498 at 2578.835 kb on + strand, within Synpcc7942_2498 at 2578.870 kb on + strand, within Synpcc7942_2498 at 2578.878 kb on - strand, within Synpcc7942_2498 at 2578.883 kb on - strand, within Synpcc7942_2498
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite 10 mM remove 2,575,692 - +1.5 2,575,716 + +2.7 2,575,724 - -0.2 2,575,732 + +1.0 2,575,743 + -1.7 2,575,743 + -0.4 2,575,743 + -0.6 2,575,744 + +1.3 2,575,744 + -0.7 2,575,744 + +0.4 2,575,744 + +0.3 2,575,749 + -0.1 2,575,751 - -1.7 2,575,752 - -0.1 2,575,752 - -0.0 2,575,752 - +0.3 2,575,752 - +0.7 2,575,767 - +0.9 2,575,776 - -1.7 2,575,776 - -1.4 2,575,776 - +0.6 2,575,776 - -2.7 2,575,786 - -1.3 2,575,787 - -1.1 2,575,799 - -1.0 2,575,799 - +0.0 2,575,799 - -4.0 2,575,800 - -0.6 2,575,804 + +0.2 2,575,812 - -1.7 2,575,821 + +0.7 2,575,822 - +0.4 2,575,846 - natD Synpcc7942_2495 0.11 +0.0 2,575,884 - natD Synpcc7942_2495 0.15 +2.8 2,575,897 + natD Synpcc7942_2495 0.16 +0.8 2,575,923 + natD Synpcc7942_2495 0.19 -0.7 2,575,923 + natD Synpcc7942_2495 0.19 -0.1 2,575,932 - natD Synpcc7942_2495 0.20 +0.9 2,575,937 - natD Synpcc7942_2495 0.20 +2.1 2,575,946 + natD Synpcc7942_2495 0.21 +0.3 2,575,991 + natD Synpcc7942_2495 0.26 -0.3 2,576,000 + natD Synpcc7942_2495 0.27 +0.3 2,576,092 - natD Synpcc7942_2495 0.37 -1.4 2,576,113 + natD Synpcc7942_2495 0.39 -0.1 2,576,129 - natD Synpcc7942_2495 0.41 +0.7 2,576,148 - natD Synpcc7942_2495 0.43 +3.0 2,576,149 + natD Synpcc7942_2495 0.43 +0.1 2,576,161 + natD Synpcc7942_2495 0.44 -1.7 2,576,196 + natD Synpcc7942_2495 0.48 +0.0 2,576,196 + natD Synpcc7942_2495 0.48 -0.1 2,576,196 + natD Synpcc7942_2495 0.48 +0.6 2,576,204 - natD Synpcc7942_2495 0.49 +0.5 2,576,204 - natD Synpcc7942_2495 0.49 +0.7 2,576,204 - natD Synpcc7942_2495 0.49 -0.7 2,576,204 - natD Synpcc7942_2495 0.49 -1.7 2,576,214 - natD Synpcc7942_2495 0.50 +0.3 2,576,214 - natD Synpcc7942_2495 0.50 -0.7 2,576,219 - natD Synpcc7942_2495 0.50 +0.9 2,576,219 - natD Synpcc7942_2495 0.50 +0.1 2,576,222 + natD Synpcc7942_2495 0.51 -1.3 2,576,230 - natD Synpcc7942_2495 0.51 -0.0 2,576,243 - natD Synpcc7942_2495 0.53 -0.7 2,576,249 - natD Synpcc7942_2495 0.53 +0.6 2,576,253 + natD Synpcc7942_2495 0.54 +0.4 2,576,253 + natD Synpcc7942_2495 0.54 +1.9 2,576,253 + natD Synpcc7942_2495 0.54 -0.7 2,576,253 + natD Synpcc7942_2495 0.54 +1.1 2,576,256 - -1.7 2,576,258 - natD Synpcc7942_2495 0.54 +1.0 2,576,261 - natD Synpcc7942_2495 0.55 +0.9 2,576,261 - natD Synpcc7942_2495 0.55 -0.3 2,576,261 - natD Synpcc7942_2495 0.55 -4.0 2,576,263 + natD Synpcc7942_2495 0.55 +0.4 2,576,270 - -0.1 2,576,271 - natD Synpcc7942_2495 0.56 +0.4 2,576,282 - natD Synpcc7942_2495 0.57 -2.3 2,576,282 - natD Synpcc7942_2495 0.57 -0.7 2,576,284 + natD Synpcc7942_2495 0.57 +1.2 2,576,357 + natD Synpcc7942_2495 0.65 -0.5 2,576,364 - natD Synpcc7942_2495 0.66 -0.3 2,576,364 - natD Synpcc7942_2495 0.66 +2.2 2,576,364 - natD Synpcc7942_2495 0.66 -0.4 2,576,392 + natD Synpcc7942_2495 0.69 -2.7 2,576,413 + natD Synpcc7942_2495 0.71 +1.2 2,576,413 + natD Synpcc7942_2495 0.71 -0.3 2,576,464 - natD Synpcc7942_2495 0.76 -0.2 2,576,476 + natD Synpcc7942_2495 0.77 +1.4 2,576,479 - natD Synpcc7942_2495 0.78 +1.4 2,576,484 - natD Synpcc7942_2495 0.78 +0.3 2,576,484 - natD Synpcc7942_2495 0.78 +0.8 2,576,486 + natD Synpcc7942_2495 0.79 +0.4 2,576,536 + natD Synpcc7942_2495 0.84 -0.4 2,576,544 - natD Synpcc7942_2495 0.85 -1.7 2,576,592 + natD Synpcc7942_2495 0.90 -1.7 2,576,613 + -0.1 2,576,615 - +1.0 2,576,621 - +0.1 2,576,622 - -3.3 2,576,641 - -0.7 2,576,641 - -1.7 2,576,641 - -1.0 2,576,657 + -2.7 2,576,657 + +0.8 2,576,667 - +0.9 2,576,698 + +0.5 2,576,698 + +0.7 2,576,707 + -1.1 2,576,747 + +0.0 2,576,750 - +1.3 2,576,760 - +1.1 2,576,775 + -0.7 2,576,776 + -1.7 2,576,776 + +0.9 2,576,776 + -0.7 2,576,776 + +1.6 2,576,784 - +1.0 2,576,792 + +0.6 2,576,800 - +0.8 2,576,800 - -0.4 2,576,803 + +0.6 2,576,803 + +0.0 2,576,821 + livJ Synpcc7942_2496 0.11 +1.1 2,576,831 + livJ Synpcc7942_2496 0.12 +0.4 2,576,838 + livJ Synpcc7942_2496 0.13 +1.1 2,576,838 + livJ Synpcc7942_2496 0.13 +2.9 2,576,838 + livJ Synpcc7942_2496 0.13 +0.3 2,576,839 - livJ Synpcc7942_2496 0.13 +0.8 2,576,846 - livJ Synpcc7942_2496 0.13 +2.2 2,576,846 - livJ Synpcc7942_2496 0.13 +1.1 2,576,846 - livJ Synpcc7942_2496 0.13 +2.0 2,576,846 - livJ Synpcc7942_2496 0.13 +2.7 2,576,846 - livJ Synpcc7942_2496 0.13 +1.2 2,576,864 + livJ Synpcc7942_2496 0.15 +1.2 2,576,864 + livJ Synpcc7942_2496 0.15 +4.0 2,576,865 - livJ Synpcc7942_2496 0.15 +1.0 2,576,866 - livJ Synpcc7942_2496 0.15 +0.5 2,576,900 - livJ Synpcc7942_2496 0.18 +1.8 2,576,912 + livJ Synpcc7942_2496 0.19 +0.3 2,576,920 - livJ Synpcc7942_2496 0.19 +0.3 2,576,920 - livJ Synpcc7942_2496 0.19 -0.2 2,576,920 - livJ Synpcc7942_2496 0.19 +0.6 2,576,959 - livJ Synpcc7942_2496 0.23 +0.3 2,576,973 + livJ Synpcc7942_2496 0.24 +0.5 2,576,998 - livJ Synpcc7942_2496 0.26 +1.8 2,577,021 + livJ Synpcc7942_2496 0.28 +1.7 2,577,048 + livJ Synpcc7942_2496 0.30 -0.9 2,577,066 - livJ Synpcc7942_2496 0.32 +1.2 2,577,091 + livJ Synpcc7942_2496 0.34 -1.7 2,577,116 + livJ Synpcc7942_2496 0.36 -1.4 2,577,116 + livJ Synpcc7942_2496 0.36 +0.6 2,577,165 + livJ Synpcc7942_2496 0.40 +1.5 2,577,166 + livJ Synpcc7942_2496 0.40 +1.1 2,577,193 - livJ Synpcc7942_2496 0.42 +0.2 2,577,213 - livJ Synpcc7942_2496 0.44 +0.2 2,577,246 + livJ Synpcc7942_2496 0.47 +0.1 2,577,254 - livJ Synpcc7942_2496 0.47 -0.3 2,577,260 - livJ Synpcc7942_2496 0.48 +2.0 2,577,277 - livJ Synpcc7942_2496 0.49 +0.2 2,577,277 - livJ Synpcc7942_2496 0.49 +0.5 2,577,356 - livJ Synpcc7942_2496 0.56 +1.1 2,577,376 + livJ Synpcc7942_2496 0.57 +0.9 2,577,387 + livJ Synpcc7942_2496 0.58 +1.2 2,577,395 + livJ Synpcc7942_2496 0.59 +1.6 2,577,401 + livJ Synpcc7942_2496 0.59 -0.1 2,577,403 - livJ Synpcc7942_2496 0.60 +0.2 2,577,423 + livJ Synpcc7942_2496 0.61 +2.2 2,577,424 - livJ Synpcc7942_2496 0.61 +0.3 2,577,482 + livJ Synpcc7942_2496 0.66 -0.5 2,577,611 + livJ Synpcc7942_2496 0.77 +2.7 2,577,619 - livJ Synpcc7942_2496 0.78 -0.9 2,577,619 - livJ Synpcc7942_2496 0.78 -0.1 2,577,632 - livJ Synpcc7942_2496 0.79 +0.8 2,577,668 - livJ Synpcc7942_2496 0.82 -3.7 2,577,670 + livJ Synpcc7942_2496 0.82 -2.7 2,577,688 - livJ Synpcc7942_2496 0.83 -0.5 2,577,701 + livJ Synpcc7942_2496 0.84 +1.4 2,577,717 - livJ Synpcc7942_2496 0.86 +1.3 2,577,722 - livJ Synpcc7942_2496 0.86 -0.1 2,577,755 + livJ Synpcc7942_2496 0.89 -2.7 2,577,763 - livJ Synpcc7942_2496 0.90 +1.1 2,577,763 - livJ Synpcc7942_2496 0.90 -1.2 2,577,769 - +1.1 2,577,791 + +0.2 2,577,829 - -0.1 2,577,834 - -0.4 2,577,835 + -0.0 2,577,835 + -0.7 2,577,843 - +1.0 2,577,848 + +1.6 2,577,856 - +0.8 2,577,858 + +1.4 2,577,889 - +0.7 2,577,907 - +0.1 2,578,369 + -3.3 2,578,369 + -1.7 2,578,369 + -4.3 2,578,369 + -0.6 2,578,381 + -0.1 2,578,382 - -2.3 2,578,397 + -1.7 2,578,405 - -1.3 2,578,419 - -0.2 2,578,438 + -1.7 2,578,449 + +0.4 2,578,467 - -0.7 2,578,468 + -0.7 2,578,468 + +1.9 2,578,477 - +1.1 2,578,477 - -0.1 2,578,477 - -0.4 2,578,495 + -0.7 2,578,579 + -0.7 2,578,579 + +2.1 2,578,587 - -1.7 2,578,601 + Synpcc7942_2498 0.10 -2.7 2,578,621 + Synpcc7942_2498 0.12 +0.4 2,578,631 + Synpcc7942_2498 0.13 +0.6 2,578,631 + Synpcc7942_2498 0.13 +0.3 2,578,639 - Synpcc7942_2498 0.13 +1.4 2,578,682 - Synpcc7942_2498 0.17 -0.5 2,578,712 + Synpcc7942_2498 0.19 +0.6 2,578,777 + Synpcc7942_2498 0.25 -0.7 2,578,801 + Synpcc7942_2498 0.27 +1.3 2,578,811 + Synpcc7942_2498 0.27 -0.7 2,578,815 + Synpcc7942_2498 0.28 +0.2 2,578,833 - Synpcc7942_2498 0.29 +0.7 2,578,834 + Synpcc7942_2498 0.29 +1.1 2,578,835 + Synpcc7942_2498 0.29 +1.6 2,578,870 + Synpcc7942_2498 0.32 +0.4 2,578,878 - Synpcc7942_2498 0.33 +1.5 2,578,883 - Synpcc7942_2498 0.33 -0.0
Or see this region's nucleotide sequence