Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_988 and Pf1N1B4_989 are separated by 11 nucleotides Pf1N1B4_989 and Pf1N1B4_990 are separated by 189 nucleotides
Pf1N1B4_988 - Alginate biosynthesis protein AlgX, at 1,118,950 to 1,120,410
_988
Pf1N1B4_989 - Alginate lyase precursor (EC 4.2.2.3), at 1,120,422 to 1,121,555
_989
Pf1N1B4_990 - Probable poly(beta-D-mannuronate) O-acetylase (EC 2.3.1.-), at 1,121,745 to 1,123,310
_990
Position (kb)
1120
1121
1122 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1119.481 kb on + strand, within Pf1N1B4_988 at 1119.481 kb on + strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.482 kb on - strand, within Pf1N1B4_988 at 1119.511 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.603 kb on + strand, within Pf1N1B4_988 at 1119.604 kb on - strand, within Pf1N1B4_988 at 1119.635 kb on + strand at 1119.635 kb on + strand, within Pf1N1B4_988 at 1119.682 kb on + strand, within Pf1N1B4_988 at 1119.682 kb on + strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.683 kb on - strand, within Pf1N1B4_988 at 1119.763 kb on - strand, within Pf1N1B4_988 at 1119.796 kb on + strand, within Pf1N1B4_988 at 1119.796 kb on + strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.797 kb on - strand, within Pf1N1B4_988 at 1119.889 kb on + strand, within Pf1N1B4_988 at 1119.890 kb on - strand, within Pf1N1B4_988 at 1119.979 kb on + strand, within Pf1N1B4_988 at 1119.979 kb on + strand, within Pf1N1B4_988 at 1120.174 kb on + strand, within Pf1N1B4_988 at 1120.175 kb on - strand, within Pf1N1B4_988 at 1120.323 kb on + strand at 1120.323 kb on + strand at 1120.414 kb on + strand at 1120.414 kb on + strand at 1120.421 kb on + strand at 1120.422 kb on - strand at 1120.422 kb on - strand at 1120.456 kb on + strand at 1120.536 kb on + strand, within Pf1N1B4_989 at 1120.536 kb on + strand, within Pf1N1B4_989 at 1120.606 kb on - strand, within Pf1N1B4_989 at 1120.635 kb on + strand, within Pf1N1B4_989 at 1120.635 kb on + strand, within Pf1N1B4_989 at 1120.635 kb on + strand at 1120.636 kb on - strand, within Pf1N1B4_989 at 1120.899 kb on + strand, within Pf1N1B4_989 at 1120.899 kb on + strand, within Pf1N1B4_989 at 1120.900 kb on - strand, within Pf1N1B4_989 at 1120.900 kb on - strand, within Pf1N1B4_989 at 1121.046 kb on + strand, within Pf1N1B4_989 at 1121.046 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.124 kb on + strand, within Pf1N1B4_989 at 1121.125 kb on - strand, within Pf1N1B4_989 at 1121.202 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.211 kb on + strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.212 kb on - strand, within Pf1N1B4_989 at 1121.360 kb on + strand, within Pf1N1B4_989 at 1121.360 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.430 kb on + strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.431 kb on - strand, within Pf1N1B4_989 at 1121.518 kb on - strand at 1121.554 kb on - strand at 1121.672 kb on + strand at 1121.672 kb on + strand at 1121.673 kb on - strand at 1121.749 kb on + strand at 1121.749 kb on + strand at 1121.749 kb on + strand at 1121.750 kb on - strand at 1121.750 kb on - strand at 1121.750 kb on - strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.802 kb on + strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.803 kb on - strand at 1121.805 kb on + strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.806 kb on - strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.853 kb on + strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.854 kb on - strand at 1121.863 kb on - strand at 1121.863 kb on - strand at 1121.919 kb on + strand, within Pf1N1B4_990 at 1121.919 kb on + strand, within Pf1N1B4_990 at 1121.920 kb on - strand, within Pf1N1B4_990 at 1122.028 kb on - strand, within Pf1N1B4_990 at 1122.129 kb on + strand, within Pf1N1B4_990 at 1122.129 kb on + strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.130 kb on - strand, within Pf1N1B4_990 at 1122.150 kb on + strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.151 kb on - strand, within Pf1N1B4_990 at 1122.166 kb on - strand, within Pf1N1B4_990 at 1122.447 kb on + strand, within Pf1N1B4_990 at 1122.448 kb on - strand at 1122.462 kb on + strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.463 kb on - strand, within Pf1N1B4_990 at 1122.480 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.549 kb on + strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990 at 1122.550 kb on - strand, within Pf1N1B4_990
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 1,119,481 + Pf1N1B4_988 0.36 -0.1 1,119,481 + Pf1N1B4_988 0.36 -1.9 1,119,482 - Pf1N1B4_988 0.36 -0.2 1,119,482 - Pf1N1B4_988 0.36 +0.1 1,119,482 - Pf1N1B4_988 0.36 +1.0 1,119,511 + Pf1N1B4_988 0.38 +1.1 1,119,603 + Pf1N1B4_988 0.45 +1.7 1,119,603 + Pf1N1B4_988 0.45 -1.3 1,119,603 + Pf1N1B4_988 0.45 -1.2 1,119,603 + Pf1N1B4_988 0.45 -1.3 1,119,604 - Pf1N1B4_988 0.45 +0.7 1,119,635 + +1.1 1,119,635 + Pf1N1B4_988 0.47 +0.0 1,119,682 + Pf1N1B4_988 0.50 +0.1 1,119,682 + Pf1N1B4_988 0.50 +0.6 1,119,683 - Pf1N1B4_988 0.50 -0.7 1,119,683 - Pf1N1B4_988 0.50 -0.3 1,119,683 - Pf1N1B4_988 0.50 -0.4 1,119,683 - Pf1N1B4_988 0.50 -1.0 1,119,683 - Pf1N1B4_988 0.50 -0.1 1,119,683 - Pf1N1B4_988 0.50 +1.9 1,119,763 - Pf1N1B4_988 0.56 +0.6 1,119,796 + Pf1N1B4_988 0.58 +1.1 1,119,796 + Pf1N1B4_988 0.58 -0.7 1,119,797 - Pf1N1B4_988 0.58 -2.7 1,119,797 - Pf1N1B4_988 0.58 -0.0 1,119,797 - Pf1N1B4_988 0.58 -1.4 1,119,797 - Pf1N1B4_988 0.58 -0.3 1,119,889 + Pf1N1B4_988 0.64 +1.1 1,119,890 - Pf1N1B4_988 0.64 +0.8 1,119,979 + Pf1N1B4_988 0.70 -1.2 1,119,979 + Pf1N1B4_988 0.70 -1.2 1,120,174 + Pf1N1B4_988 0.84 -0.0 1,120,175 - Pf1N1B4_988 0.84 -0.3 1,120,323 + -0.5 1,120,323 + +0.9 1,120,414 + +3.5 1,120,414 + -0.7 1,120,421 + +1.6 1,120,422 - +0.6 1,120,422 - -0.2 1,120,456 + +0.6 1,120,536 + Pf1N1B4_989 0.10 +1.3 1,120,536 + Pf1N1B4_989 0.10 +0.1 1,120,606 - Pf1N1B4_989 0.16 +0.3 1,120,635 + Pf1N1B4_989 0.19 -1.2 1,120,635 + Pf1N1B4_989 0.19 +1.1 1,120,635 + +1.1 1,120,636 - Pf1N1B4_989 0.19 +1.7 1,120,899 + Pf1N1B4_989 0.42 +1.4 1,120,899 + Pf1N1B4_989 0.42 -0.2 1,120,900 - Pf1N1B4_989 0.42 +0.3 1,120,900 - Pf1N1B4_989 0.42 -0.4 1,121,046 + Pf1N1B4_989 0.55 -1.2 1,121,046 + Pf1N1B4_989 0.55 +1.5 1,121,124 + Pf1N1B4_989 0.62 -0.6 1,121,124 + Pf1N1B4_989 0.62 -1.4 1,121,124 + Pf1N1B4_989 0.62 +0.1 1,121,124 + Pf1N1B4_989 0.62 +1.7 1,121,124 + Pf1N1B4_989 0.62 -0.4 1,121,124 + Pf1N1B4_989 0.62 +0.9 1,121,125 - Pf1N1B4_989 0.62 +0.7 1,121,202 + Pf1N1B4_989 0.69 -0.9 1,121,211 + Pf1N1B4_989 0.70 +1.6 1,121,211 + Pf1N1B4_989 0.70 +0.1 1,121,211 + Pf1N1B4_989 0.70 -0.2 1,121,211 + Pf1N1B4_989 0.70 +1.6 1,121,211 + Pf1N1B4_989 0.70 +1.5 1,121,211 + Pf1N1B4_989 0.70 +0.6 1,121,212 - Pf1N1B4_989 0.70 +1.3 1,121,212 - Pf1N1B4_989 0.70 -1.9 1,121,212 - Pf1N1B4_989 0.70 +1.1 1,121,212 - Pf1N1B4_989 0.70 -0.4 1,121,360 + Pf1N1B4_989 0.83 +0.3 1,121,360 + Pf1N1B4_989 0.83 -0.4 1,121,430 + Pf1N1B4_989 0.89 +1.7 1,121,430 + Pf1N1B4_989 0.89 +2.1 1,121,430 + Pf1N1B4_989 0.89 -0.3 1,121,431 - Pf1N1B4_989 0.89 -1.4 1,121,431 - Pf1N1B4_989 0.89 +0.6 1,121,431 - Pf1N1B4_989 0.89 +0.4 1,121,431 - Pf1N1B4_989 0.89 -0.4 1,121,518 - -0.5 1,121,554 - +0.1 1,121,672 + +0.3 1,121,672 + +0.1 1,121,673 - -1.2 1,121,749 + -1.4 1,121,749 + -0.7 1,121,749 + -0.2 1,121,750 - -0.8 1,121,750 - +0.1 1,121,750 - -1.4 1,121,802 + +0.1 1,121,802 + -1.9 1,121,802 + -1.2 1,121,802 + +0.1 1,121,802 + +0.4 1,121,803 - -1.4 1,121,803 - -0.1 1,121,803 - -0.7 1,121,803 - -2.0 1,121,805 + -1.2 1,121,806 - -0.1 1,121,806 - +0.3 1,121,806 - -0.7 1,121,806 - +1.9 1,121,806 - -1.9 1,121,806 - +0.5 1,121,806 - -0.4 1,121,806 - +0.4 1,121,853 + +1.7 1,121,853 + +1.1 1,121,853 + -0.9 1,121,853 + -1.7 1,121,853 + -0.4 1,121,854 - -0.9 1,121,854 - -0.9 1,121,854 - +0.6 1,121,854 - -0.4 1,121,854 - -1.1 1,121,863 - +0.4 1,121,863 - +0.7 1,121,919 + Pf1N1B4_990 0.11 +0.1 1,121,919 + Pf1N1B4_990 0.11 +1.1 1,121,920 - Pf1N1B4_990 0.11 -0.2 1,122,028 - Pf1N1B4_990 0.18 -0.3 1,122,129 + Pf1N1B4_990 0.25 +0.7 1,122,129 + Pf1N1B4_990 0.25 +0.1 1,122,130 - Pf1N1B4_990 0.25 -2.6 1,122,130 - Pf1N1B4_990 0.25 -0.0 1,122,130 - Pf1N1B4_990 0.25 +0.1 1,122,150 + Pf1N1B4_990 0.26 -0.2 1,122,151 - Pf1N1B4_990 0.26 -0.8 1,122,151 - Pf1N1B4_990 0.26 +0.8 1,122,151 - Pf1N1B4_990 0.26 -0.1 1,122,151 - Pf1N1B4_990 0.26 -0.4 1,122,151 - Pf1N1B4_990 0.26 +0.4 1,122,151 - Pf1N1B4_990 0.26 -2.9 1,122,151 - Pf1N1B4_990 0.26 +1.1 1,122,151 - Pf1N1B4_990 0.26 -1.4 1,122,166 - Pf1N1B4_990 0.27 +0.1 1,122,447 + Pf1N1B4_990 0.45 +0.1 1,122,448 - +0.8 1,122,462 + Pf1N1B4_990 0.46 +0.1 1,122,463 - Pf1N1B4_990 0.46 +0.9 1,122,463 - Pf1N1B4_990 0.46 +0.6 1,122,463 - Pf1N1B4_990 0.46 -0.9 1,122,463 - Pf1N1B4_990 0.46 +0.1 1,122,463 - Pf1N1B4_990 0.46 -0.4 1,122,480 + Pf1N1B4_990 0.47 +2.1 1,122,549 + Pf1N1B4_990 0.51 +0.7 1,122,549 + Pf1N1B4_990 0.51 +0.5 1,122,549 + Pf1N1B4_990 0.51 -1.4 1,122,549 + Pf1N1B4_990 0.51 -0.2 1,122,549 + Pf1N1B4_990 0.51 +0.7 1,122,550 - Pf1N1B4_990 0.51 -1.0 1,122,550 - Pf1N1B4_990 0.51 +0.1 1,122,550 - Pf1N1B4_990 0.51 +0.3 1,122,550 - Pf1N1B4_990 0.51 -0.9 1,122,550 - Pf1N1B4_990 0.51 +0.5
Or see this region's nucleotide sequence