Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5930 and Pf1N1B4_5931 are separated by 141 nucleotides Pf1N1B4_5931 and Pf1N1B4_5932 overlap by 4 nucleotides
Pf1N1B4_5930 - Exopolysaccharide production protein ExoZ, at 375,011 to 376,126
_5930
Pf1N1B4_5931 - hypothetical protein, at 376,268 to 376,627
_5931
Pf1N1B4_5932 - YjbH outer membrane lipoprotein, at 376,624 to 378,726
_5932
Position (kb) 375
376
377 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 374.024 kb on - strand at 374.048 kb on - strand at 374.102 kb on - strand at 374.102 kb on - strand at 374.102 kb on - strand at 374.102 kb on - strand at 374.104 kb on - strand at 374.104 kb on - strand at 374.104 kb on - strand at 374.352 kb on - strand at 374.352 kb on - strand at 374.352 kb on - strand at 374.416 kb on - strand at 374.510 kb on - strand at 374.570 kb on - strand at 374.728 kb on - strand at 374.801 kb on + strand at 374.910 kb on + strand at 374.911 kb on - strand at 374.945 kb on + strand at 374.945 kb on + strand at 374.945 kb on + strand at 374.945 kb on + strand at 374.960 kb on + strand at 374.960 kb on + strand at 375.013 kb on - strand at 375.071 kb on - strand at 375.107 kb on + strand at 375.108 kb on - strand at 375.117 kb on + strand at 375.117 kb on + strand at 375.118 kb on - strand at 375.197 kb on + strand, within Pf1N1B4_5930 at 375.198 kb on - strand, within Pf1N1B4_5930 at 375.241 kb on - strand, within Pf1N1B4_5930 at 375.241 kb on - strand, within Pf1N1B4_5930 at 375.250 kb on - strand, within Pf1N1B4_5930 at 375.276 kb on - strand, within Pf1N1B4_5930 at 375.276 kb on - strand, within Pf1N1B4_5930 at 375.290 kb on - strand, within Pf1N1B4_5930 at 375.290 kb on - strand, within Pf1N1B4_5930 at 375.357 kb on - strand, within Pf1N1B4_5930 at 375.357 kb on - strand, within Pf1N1B4_5930 at 375.410 kb on + strand, within Pf1N1B4_5930 at 375.413 kb on - strand, within Pf1N1B4_5930 at 375.416 kb on + strand, within Pf1N1B4_5930 at 375.444 kb on + strand, within Pf1N1B4_5930 at 375.445 kb on - strand, within Pf1N1B4_5930 at 375.445 kb on - strand, within Pf1N1B4_5930 at 375.494 kb on - strand at 375.513 kb on + strand, within Pf1N1B4_5930 at 375.672 kb on - strand, within Pf1N1B4_5930 at 375.738 kb on - strand at 375.739 kb on + strand, within Pf1N1B4_5930 at 375.739 kb on + strand, within Pf1N1B4_5930 at 375.789 kb on - strand, within Pf1N1B4_5930 at 376.000 kb on - strand, within Pf1N1B4_5930 at 376.040 kb on - strand at 376.047 kb on - strand at 376.059 kb on - strand at 376.066 kb on - strand at 376.108 kb on + strand at 376.108 kb on + strand at 376.109 kb on - strand at 376.113 kb on - strand at 376.113 kb on - strand at 376.115 kb on + strand at 376.116 kb on - strand at 376.180 kb on - strand at 376.180 kb on - strand at 376.229 kb on + strand at 376.319 kb on - strand, within Pf1N1B4_5931 at 376.319 kb on - strand, within Pf1N1B4_5931
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 374,024 - -0.6 374,048 - +0.5 374,102 - +0.0 374,102 - +1.5 374,102 - +0.7 374,102 - +1.3 374,104 - -1.0 374,104 - -0.5 374,104 - -0.3 374,352 - +0.0 374,352 - -1.6 374,352 - +0.5 374,416 - +3.1 374,510 - +2.3 374,570 - +0.4 374,728 - -2.0 374,801 + -0.5 374,910 + -2.3 374,911 - -1.6 374,945 + -0.6 374,945 + -1.6 374,945 + -0.0 374,945 + -1.0 374,960 + +0.1 374,960 + +0.5 375,013 - -0.5 375,071 - -0.5 375,107 + +0.2 375,108 - +1.5 375,117 + +0.5 375,117 + +0.7 375,118 - -0.5 375,197 + Pf1N1B4_5930 0.17 +0.9 375,198 - Pf1N1B4_5930 0.17 -0.7 375,241 - Pf1N1B4_5930 0.21 +0.7 375,241 - Pf1N1B4_5930 0.21 -1.0 375,250 - Pf1N1B4_5930 0.21 +0.6 375,276 - Pf1N1B4_5930 0.24 +1.0 375,276 - Pf1N1B4_5930 0.24 +0.3 375,290 - Pf1N1B4_5930 0.25 +1.5 375,290 - Pf1N1B4_5930 0.25 +0.5 375,357 - Pf1N1B4_5930 0.31 +0.5 375,357 - Pf1N1B4_5930 0.31 +0.5 375,410 + Pf1N1B4_5930 0.36 +0.5 375,413 - Pf1N1B4_5930 0.36 +1.4 375,416 + Pf1N1B4_5930 0.36 +0.2 375,444 + Pf1N1B4_5930 0.39 +0.9 375,445 - Pf1N1B4_5930 0.39 -0.3 375,445 - Pf1N1B4_5930 0.39 +1.1 375,494 - -0.5 375,513 + Pf1N1B4_5930 0.45 -0.9 375,672 - Pf1N1B4_5930 0.59 +1.2 375,738 - -0.8 375,739 + Pf1N1B4_5930 0.65 -1.5 375,739 + Pf1N1B4_5930 0.65 -0.2 375,789 - Pf1N1B4_5930 0.70 -0.3 376,000 - Pf1N1B4_5930 0.89 +2.4 376,040 - +2.7 376,047 - -1.6 376,059 - +0.6 376,066 - +0.5 376,108 + +1.2 376,108 + +0.9 376,109 - +0.9 376,113 - +0.5 376,113 - +1.3 376,115 + +1.0 376,116 - +0.9 376,180 - -0.5 376,180 - -2.0 376,229 + +1.0 376,319 - Pf1N1B4_5931 0.14 +0.5 376,319 - Pf1N1B4_5931 0.14 -0.0
Or see this region's nucleotide sequence