Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5429 and Pf1N1B4_5430 are separated by 85 nucleotides Pf1N1B4_5430 and Pf1N1B4_5431 are separated by 720 nucleotides Pf1N1B4_5431 and Pf1N1B4_5432 are separated by 94 nucleotides
Pf1N1B4_5429 - hypothetical protein, at 168,899 to 169,543
_5429
Pf1N1B4_5430 - hypothetical protein, at 169,629 to 169,796
_5430
Pf1N1B4_5431 - hypothetical protein, at 170,517 to 171,065
_5431
Pf1N1B4_5432 - hypothetical protein, at 171,160 to 171,348
_5432
Position (kb) 170
171
172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.549 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.584 kb on + strand at 169.585 kb on - strand at 169.585 kb on - strand at 169.631 kb on - strand at 169.681 kb on + strand, within Pf1N1B4_5430 at 169.682 kb on - strand, within Pf1N1B4_5430 at 169.747 kb on - strand at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.750 kb on + strand, within Pf1N1B4_5430 at 169.751 kb on - strand at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.751 kb on - strand, within Pf1N1B4_5430 at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.792 kb on + strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.793 kb on - strand at 169.809 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.895 kb on + strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.896 kb on - strand at 169.899 kb on + strand at 169.899 kb on + strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.900 kb on - strand at 169.911 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.016 kb on + strand at 170.017 kb on - strand at 170.069 kb on + strand at 170.082 kb on + strand at 170.083 kb on - strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.156 kb on + strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.157 kb on - strand at 170.190 kb on - strand at 170.190 kb on - strand at 170.244 kb on + strand at 170.244 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.256 kb on + strand at 170.282 kb on + strand at 170.368 kb on + strand at 170.368 kb on + strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.369 kb on - strand at 170.553 kb on + strand at 170.554 kb on - strand at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.620 kb on + strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.621 kb on - strand at 170.621 kb on - strand, within Pf1N1B4_5431 at 170.739 kb on - strand, within Pf1N1B4_5431 at 170.746 kb on + strand, within Pf1N1B4_5431 at 170.746 kb on + strand, within Pf1N1B4_5431 at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.747 kb on - strand at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.747 kb on - strand, within Pf1N1B4_5431 at 170.899 kb on + strand, within Pf1N1B4_5431 at 170.899 kb on + strand at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.900 kb on - strand, within Pf1N1B4_5431 at 170.956 kb on + strand, within Pf1N1B4_5431 at 171.076 kb on + strand at 171.100 kb on - strand at 171.109 kb on + strand at 171.109 kb on + strand at 171.109 kb on + strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.110 kb on - strand at 171.158 kb on - strand at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.229 kb on + strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.230 kb on - strand, within Pf1N1B4_5432 at 171.235 kb on + strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.291 kb on - strand, within Pf1N1B4_5432 at 171.365 kb on - strand at 171.416 kb on + strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.561 kb on - strand at 171.585 kb on - strand at 171.667 kb on - strand at 171.705 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.729 kb on + strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.730 kb on - strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.733 kb on + strand at 171.734 kb on - strand at 171.756 kb on - strand at 171.761 kb on + strand at 171.762 kb on - strand at 171.762 kb on - strand at 171.849 kb on - strand at 171.855 kb on + strand at 171.887 kb on - strand at 171.892 kb on + strand at 171.954 kb on + strand at 171.954 kb on + strand at 171.965 kb on + strand at 171.966 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 169,549 + +0.9 169,549 + -0.2 169,549 + -0.5 169,549 + +0.8 169,549 + +0.8 169,584 + +2.9 169,584 + -1.6 169,584 + +0.4 169,585 - +0.8 169,585 - +0.1 169,631 - -0.7 169,681 + Pf1N1B4_5430 0.31 -0.1 169,682 - Pf1N1B4_5430 0.32 +1.3 169,747 - +0.5 169,750 + Pf1N1B4_5430 0.72 +1.1 169,750 + Pf1N1B4_5430 0.72 -0.5 169,751 - -0.9 169,751 - Pf1N1B4_5430 0.73 -0.5 169,751 - Pf1N1B4_5430 0.73 +0.1 169,751 - Pf1N1B4_5430 0.73 -1.2 169,751 - Pf1N1B4_5430 0.73 -0.9 169,792 + -1.5 169,792 + -1.1 169,792 + +0.1 169,792 + +0.1 169,793 - -0.4 169,793 - -0.9 169,793 - -0.1 169,793 - -2.7 169,793 - -0.1 169,809 + -0.1 169,895 + -0.7 169,895 + +0.1 169,895 + -0.7 169,895 + -0.2 169,895 + -0.5 169,896 - +0.4 169,896 - +0.7 169,896 - -1.1 169,896 - -0.9 169,896 - +1.4 169,896 - +0.7 169,896 - -0.7 169,896 - -1.2 169,899 + -0.9 169,899 + -1.7 169,900 - -0.2 169,900 - -1.3 169,900 - +0.8 169,911 + -0.1 170,016 + +0.5 170,016 + -0.5 170,016 + -0.1 170,017 - -1.2 170,069 + +1.5 170,082 + -0.9 170,083 - +0.5 170,156 + +1.1 170,156 + -2.1 170,156 + +0.0 170,157 - -0.2 170,157 - -1.1 170,157 - -0.9 170,157 - +0.4 170,190 - +1.1 170,190 - -0.1 170,244 + +2.1 170,244 + -0.5 170,256 + -2.5 170,256 + -0.6 170,256 + -0.2 170,282 + +1.1 170,368 + +0.3 170,368 + -0.3 170,369 - +0.7 170,369 - +0.9 170,369 - +1.7 170,369 - -0.5 170,369 - +0.7 170,553 + +1.1 170,554 - +1.1 170,620 + Pf1N1B4_5431 0.19 +1.4 170,620 + Pf1N1B4_5431 0.19 +1.5 170,620 + Pf1N1B4_5431 0.19 -0.1 170,620 + Pf1N1B4_5431 0.19 +0.2 170,620 + Pf1N1B4_5431 0.19 +0.3 170,620 + Pf1N1B4_5431 0.19 +0.2 170,620 + Pf1N1B4_5431 0.19 +0.4 170,620 + Pf1N1B4_5431 0.19 +2.1 170,620 + Pf1N1B4_5431 0.19 +1.1 170,620 + Pf1N1B4_5431 0.19 -0.9 170,621 - Pf1N1B4_5431 0.19 +1.9 170,621 - Pf1N1B4_5431 0.19 -0.5 170,621 - Pf1N1B4_5431 0.19 +2.1 170,621 - Pf1N1B4_5431 0.19 -1.7 170,621 - Pf1N1B4_5431 0.19 +1.5 170,621 - Pf1N1B4_5431 0.19 +1.5 170,621 - +0.1 170,621 - Pf1N1B4_5431 0.19 +0.6 170,621 - +1.1 170,621 - Pf1N1B4_5431 0.19 +0.1 170,739 - Pf1N1B4_5431 0.40 +0.1 170,746 + Pf1N1B4_5431 0.42 -0.5 170,746 + Pf1N1B4_5431 0.42 +0.8 170,747 - Pf1N1B4_5431 0.42 +1.7 170,747 - -0.5 170,747 - Pf1N1B4_5431 0.42 -0.1 170,747 - Pf1N1B4_5431 0.42 +1.3 170,899 + Pf1N1B4_5431 0.70 +0.1 170,899 + +0.1 170,900 - Pf1N1B4_5431 0.70 +1.8 170,900 - Pf1N1B4_5431 0.70 -1.1 170,900 - Pf1N1B4_5431 0.70 -1.2 170,956 + Pf1N1B4_5431 0.80 +1.3 171,076 + -0.2 171,100 - +0.4 171,109 + -0.9 171,109 + -1.1 171,109 + +1.8 171,110 - -0.8 171,110 - +0.1 171,110 - +0.4 171,110 - +0.1 171,158 - +0.5 171,229 + Pf1N1B4_5432 0.37 -1.2 171,229 + Pf1N1B4_5432 0.37 -0.1 171,229 + Pf1N1B4_5432 0.37 -0.0 171,229 + Pf1N1B4_5432 0.37 -1.5 171,230 - Pf1N1B4_5432 0.37 -0.4 171,230 - Pf1N1B4_5432 0.37 -1.2 171,230 - Pf1N1B4_5432 0.37 -1.2 171,230 - Pf1N1B4_5432 0.37 +1.3 171,230 - Pf1N1B4_5432 0.37 -0.7 171,230 - Pf1N1B4_5432 0.37 -0.6 171,230 - Pf1N1B4_5432 0.37 +0.5 171,235 + Pf1N1B4_5432 0.40 +1.8 171,291 - Pf1N1B4_5432 0.69 -1.1 171,291 - Pf1N1B4_5432 0.69 -0.7 171,365 - +2.3 171,416 + +1.8 171,417 - -0.9 171,417 - -1.1 171,417 - -1.2 171,417 - -0.9 171,417 - +0.5 171,561 - +1.4 171,585 - +0.8 171,667 - -0.2 171,705 + -0.2 171,729 + -0.1 171,729 + -0.7 171,729 + +1.1 171,729 + +0.1 171,730 - +1.1 171,730 - +0.4 171,730 - -0.2 171,730 - +1.1 171,733 + -0.9 171,733 + -0.1 171,733 + -0.3 171,734 - -1.3 171,756 - -0.5 171,761 + +2.1 171,762 - -0.1 171,762 - +0.2 171,849 - -3.4 171,855 + -1.2 171,887 - -0.5 171,892 + +0.5 171,954 + -1.1 171,954 + -0.9 171,965 + -0.3 171,966 - -0.4
Or see this region's nucleotide sequence