Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3857 and Pf1N1B4_3858 are separated by 184 nucleotides Pf1N1B4_3858 and Pf1N1B4_3859 are separated by 554 nucleotides
Pf1N1B4_3857 - DNA ligase (EC 6.5.1.2), at 4,283,184 to 4,285,541
_3857
Pf1N1B4_3858 - FIG00954563: hypothetical protein, at 4,285,726 to 4,286,889
_3858
Pf1N1B4_3859 - Transcriptional regulator, Cro/CI family, at 4,287,444 to 4,287,764
_3859
Position (kb)
4285
4286
4287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4285.695 kb on + strand at 4285.695 kb on + strand at 4285.695 kb on + strand at 4285.729 kb on + strand at 4285.876 kb on + strand at 4286.380 kb on + strand, within Pf1N1B4_3858 at 4286.380 kb on + strand, within Pf1N1B4_3858 at 4286.380 kb on + strand, within Pf1N1B4_3858 at 4286.381 kb on - strand, within Pf1N1B4_3858 at 4286.381 kb on - strand, within Pf1N1B4_3858 at 4286.458 kb on + strand, within Pf1N1B4_3858 at 4286.459 kb on - strand, within Pf1N1B4_3858 at 4286.459 kb on - strand, within Pf1N1B4_3858 at 4286.482 kb on + strand, within Pf1N1B4_3858 at 4286.482 kb on + strand, within Pf1N1B4_3858 at 4286.518 kb on + strand, within Pf1N1B4_3858 at 4286.518 kb on + strand, within Pf1N1B4_3858 at 4286.518 kb on + strand, within Pf1N1B4_3858 at 4286.519 kb on - strand, within Pf1N1B4_3858 at 4286.887 kb on + strand at 4286.887 kb on + strand at 4286.888 kb on - strand at 4286.911 kb on + strand at 4286.911 kb on + strand at 4286.972 kb on - strand at 4287.314 kb on - strand at 4287.383 kb on + strand at 4287.449 kb on + strand at 4287.449 kb on + strand at 4287.450 kb on - strand at 4287.450 kb on - strand at 4287.450 kb on - strand at 4287.453 kb on - strand at 4287.453 kb on - strand at 4287.453 kb on - strand at 4287.453 kb on - strand at 4287.453 kb on - strand at 4287.519 kb on + strand, within Pf1N1B4_3859 at 4287.520 kb on - strand, within Pf1N1B4_3859 at 4287.520 kb on - strand, within Pf1N1B4_3859 at 4287.536 kb on - strand, within Pf1N1B4_3859 at 4287.536 kb on - strand, within Pf1N1B4_3859 at 4287.637 kb on + strand, within Pf1N1B4_3859 at 4287.637 kb on + strand at 4287.637 kb on + strand at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.638 kb on - strand, within Pf1N1B4_3859 at 4287.708 kb on - strand, within Pf1N1B4_3859 at 4287.708 kb on - strand, within Pf1N1B4_3859 at 4287.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 4,285,695 + -0.5 4,285,695 + -0.9 4,285,695 + -0.5 4,285,729 + -0.2 4,285,876 + -0.8 4,286,380 + Pf1N1B4_3858 0.56 -0.8 4,286,380 + Pf1N1B4_3858 0.56 -1.8 4,286,380 + Pf1N1B4_3858 0.56 -1.2 4,286,381 - Pf1N1B4_3858 0.56 -0.8 4,286,381 - Pf1N1B4_3858 0.56 -0.8 4,286,458 + Pf1N1B4_3858 0.63 -1.8 4,286,459 - Pf1N1B4_3858 0.63 -0.2 4,286,459 - Pf1N1B4_3858 0.63 -2.5 4,286,482 + Pf1N1B4_3858 0.65 -0.6 4,286,482 + Pf1N1B4_3858 0.65 -1.2 4,286,518 + Pf1N1B4_3858 0.68 +0.2 4,286,518 + Pf1N1B4_3858 0.68 +0.5 4,286,518 + Pf1N1B4_3858 0.68 -0.8 4,286,519 - Pf1N1B4_3858 0.68 -0.6 4,286,887 + +0.0 4,286,887 + +0.7 4,286,888 - +0.2 4,286,911 + -0.1 4,286,911 + +0.8 4,286,972 - +1.1 4,287,314 - +0.0 4,287,383 + +0.6 4,287,449 + -0.2 4,287,449 + +0.8 4,287,450 - +0.8 4,287,450 - -2.9 4,287,450 - -0.3 4,287,453 - -0.8 4,287,453 - -1.2 4,287,453 - +0.8 4,287,453 - +0.4 4,287,453 - -0.2 4,287,519 + Pf1N1B4_3859 0.23 +0.4 4,287,520 - Pf1N1B4_3859 0.24 -0.2 4,287,520 - Pf1N1B4_3859 0.24 -0.3 4,287,536 - Pf1N1B4_3859 0.29 -2.4 4,287,536 - Pf1N1B4_3859 0.29 +1.4 4,287,637 + Pf1N1B4_3859 0.60 +0.5 4,287,637 + +0.8 4,287,637 + +0.2 4,287,638 - Pf1N1B4_3859 0.60 -0.0 4,287,638 - Pf1N1B4_3859 0.60 -0.8 4,287,638 - Pf1N1B4_3859 0.60 -0.1 4,287,638 - Pf1N1B4_3859 0.60 +1.8 4,287,638 - Pf1N1B4_3859 0.60 -0.3 4,287,638 - Pf1N1B4_3859 0.60 -0.8 4,287,638 - Pf1N1B4_3859 0.60 +0.4 4,287,638 - Pf1N1B4_3859 0.60 -0.2 4,287,708 - Pf1N1B4_3859 0.82 +0.8 4,287,708 - Pf1N1B4_3859 0.82 -1.3 4,287,813 + +2.4
Or see this region's nucleotide sequence