Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3749 and Pf1N1B4_3750 are separated by 160 nucleotides Pf1N1B4_3750 and Pf1N1B4_3751 are separated by 120 nucleotides Pf1N1B4_3751 and Pf1N1B4_3752 are separated by 206 nucleotides
Pf1N1B4_3749 - 2-aminoethylphosphonate uptake and metabolism regulator, at 4,160,709 to 4,161,422
_3749
Pf1N1B4_3750 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 4,161,583 to 4,163,670
_3750
Pf1N1B4_3751 - FIG00953060: hypothetical protein, at 4,163,791 to 4,163,967
_3751
Pf1N1B4_3752 - Aspartyl aminopeptidase, at 4,164,174 to 4,165,463
_3752
Position (kb)
4161
4162
4163
4164 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4160.613 kb on + strand at 4160.613 kb on + strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.624 kb on + strand at 4160.625 kb on - strand at 4160.697 kb on + strand at 4160.863 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand at 4161.339 kb on + strand, within Pf1N1B4_3749 at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.573 kb on - strand at 4161.585 kb on - strand at 4161.585 kb on - strand at 4161.656 kb on - strand at 4162.031 kb on + strand, within Pf1N1B4_3750 at 4162.967 kb on + strand, within Pf1N1B4_3750 at 4162.967 kb on + strand, within Pf1N1B4_3750 at 4162.968 kb on - strand, within Pf1N1B4_3750 at 4163.037 kb on + strand at 4163.103 kb on - strand, within Pf1N1B4_3750 at 4163.111 kb on + strand, within Pf1N1B4_3750 at 4163.111 kb on + strand, within Pf1N1B4_3750 at 4163.111 kb on + strand, within Pf1N1B4_3750 at 4163.112 kb on - strand, within Pf1N1B4_3750 at 4163.515 kb on + strand at 4163.516 kb on - strand at 4163.751 kb on + strand at 4163.752 kb on - strand at 4163.752 kb on - strand at 4163.792 kb on + strand at 4163.792 kb on + strand at 4163.792 kb on + strand at 4163.819 kb on + strand at 4163.819 kb on + strand, within Pf1N1B4_3751 at 4163.819 kb on + strand, within Pf1N1B4_3751 at 4163.820 kb on - strand, within Pf1N1B4_3751 at 4163.820 kb on - strand at 4163.991 kb on + strand at 4164.002 kb on + strand at 4164.002 kb on + strand at 4164.104 kb on + strand at 4164.104 kb on + strand at 4164.110 kb on + strand at 4164.111 kb on - strand at 4164.140 kb on + strand at 4164.175 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.196 kb on + strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.197 kb on - strand at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.335 kb on + strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.336 kb on - strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.381 kb on + strand, within Pf1N1B4_3752 at 4164.382 kb on - strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.517 kb on + strand, within Pf1N1B4_3752 at 4164.518 kb on - strand at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.518 kb on - strand, within Pf1N1B4_3752 at 4164.579 kb on + strand, within Pf1N1B4_3752
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 4,160,613 + -0.3 4,160,613 + +1.2 4,160,614 - +0.2 4,160,614 - -1.3 4,160,614 - +1.6 4,160,624 + +0.1 4,160,625 - -1.5 4,160,697 + -1.5 4,160,863 + Pf1N1B4_3749 0.22 +2.4 4,160,940 + Pf1N1B4_3749 0.32 -0.8 4,160,940 + Pf1N1B4_3749 0.32 +0.2 4,161,129 + Pf1N1B4_3749 0.59 +1.4 4,161,129 + Pf1N1B4_3749 0.59 -1.0 4,161,129 + Pf1N1B4_3749 0.59 -1.5 4,161,129 + Pf1N1B4_3749 0.59 -0.2 4,161,129 + Pf1N1B4_3749 0.59 +0.1 4,161,129 + Pf1N1B4_3749 0.59 -0.8 4,161,129 + Pf1N1B4_3749 0.59 -0.8 4,161,129 + Pf1N1B4_3749 0.59 +0.8 4,161,130 - Pf1N1B4_3749 0.59 -0.8 4,161,130 - Pf1N1B4_3749 0.59 +1.3 4,161,130 - Pf1N1B4_3749 0.59 -1.0 4,161,130 - Pf1N1B4_3749 0.59 +0.3 4,161,130 - Pf1N1B4_3749 0.59 +0.8 4,161,141 + Pf1N1B4_3749 0.61 -0.6 4,161,141 + Pf1N1B4_3749 0.61 -1.2 4,161,207 + Pf1N1B4_3749 0.70 -1.5 4,161,207 + Pf1N1B4_3749 0.70 +0.8 4,161,208 - Pf1N1B4_3749 0.70 -0.0 4,161,208 - Pf1N1B4_3749 0.70 +0.2 4,161,208 - Pf1N1B4_3749 0.70 -0.8 4,161,208 - +0.2 4,161,339 + Pf1N1B4_3749 0.88 -1.0 4,161,528 + +1.6 4,161,528 + +3.6 4,161,528 + -0.8 4,161,528 + -2.6 4,161,528 + +0.2 4,161,528 + +0.8 4,161,528 + +1.0 4,161,528 + -0.0 4,161,528 + +1.5 4,161,529 - -0.4 4,161,529 - -0.8 4,161,529 - +1.8 4,161,529 - +1.8 4,161,573 - +0.6 4,161,585 - +0.1 4,161,585 - -1.2 4,161,656 - -0.6 4,162,031 + Pf1N1B4_3750 0.21 -1.5 4,162,967 + Pf1N1B4_3750 0.66 +2.0 4,162,967 + Pf1N1B4_3750 0.66 +1.6 4,162,968 - Pf1N1B4_3750 0.66 +0.8 4,163,037 + +0.2 4,163,103 - Pf1N1B4_3750 0.73 -1.0 4,163,111 + Pf1N1B4_3750 0.73 +0.6 4,163,111 + Pf1N1B4_3750 0.73 +1.4 4,163,111 + Pf1N1B4_3750 0.73 +1.4 4,163,112 - Pf1N1B4_3750 0.73 -2.0 4,163,515 + +2.8 4,163,516 - -0.5 4,163,751 + -2.0 4,163,752 - -0.2 4,163,752 - +0.7 4,163,792 + +0.4 4,163,792 + -0.2 4,163,792 + +1.4 4,163,819 + +0.8 4,163,819 + Pf1N1B4_3751 0.16 +0.3 4,163,819 + Pf1N1B4_3751 0.16 -0.6 4,163,820 - Pf1N1B4_3751 0.16 -2.3 4,163,820 - +0.2 4,163,991 + +0.5 4,164,002 + -1.1 4,164,002 + -0.4 4,164,104 + -1.0 4,164,104 + -0.5 4,164,110 + +0.0 4,164,111 - -0.6 4,164,140 + -1.5 4,164,175 + +2.0 4,164,196 + -1.5 4,164,196 + -0.3 4,164,196 + -0.8 4,164,196 + +0.4 4,164,197 - -0.2 4,164,197 - -0.2 4,164,197 - -1.6 4,164,197 - -2.2 4,164,197 - +0.1 4,164,335 + Pf1N1B4_3752 0.12 -0.6 4,164,335 + Pf1N1B4_3752 0.12 -0.8 4,164,335 + Pf1N1B4_3752 0.12 -0.1 4,164,335 + Pf1N1B4_3752 0.12 -0.5 4,164,336 - Pf1N1B4_3752 0.13 -0.6 4,164,336 - Pf1N1B4_3752 0.13 +0.1 4,164,336 - Pf1N1B4_3752 0.13 +0.2 4,164,336 - Pf1N1B4_3752 0.13 +0.8 4,164,381 + Pf1N1B4_3752 0.16 -0.8 4,164,381 + Pf1N1B4_3752 0.16 -0.3 4,164,381 + Pf1N1B4_3752 0.16 -0.7 4,164,382 - Pf1N1B4_3752 0.16 -0.6 4,164,517 + Pf1N1B4_3752 0.27 -0.4 4,164,517 + Pf1N1B4_3752 0.27 -0.9 4,164,517 + Pf1N1B4_3752 0.27 -1.2 4,164,517 + Pf1N1B4_3752 0.27 +0.2 4,164,517 + Pf1N1B4_3752 0.27 +0.6 4,164,517 + Pf1N1B4_3752 0.27 +1.1 4,164,518 - +1.8 4,164,518 - Pf1N1B4_3752 0.27 -1.0 4,164,518 - Pf1N1B4_3752 0.27 -0.0 4,164,518 - Pf1N1B4_3752 0.27 -0.4 4,164,518 - Pf1N1B4_3752 0.27 -1.0 4,164,518 - Pf1N1B4_3752 0.27 +0.4 4,164,518 - Pf1N1B4_3752 0.27 +0.7 4,164,518 - Pf1N1B4_3752 0.27 -0.6 4,164,579 + Pf1N1B4_3752 0.31 +2.6
Or see this region's nucleotide sequence