Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3580 and Pf1N1B4_3581 overlap by 4 nucleotides Pf1N1B4_3581 and Pf1N1B4_3582 overlap by 11 nucleotides Pf1N1B4_3582 and Pf1N1B4_3583 are separated by 16 nucleotides Pf1N1B4_3583 and Pf1N1B4_3584 are separated by 51 nucleotides
Pf1N1B4_3580 - methyltransferase, putative, at 3,990,533 to 3,991,759
_3580
Pf1N1B4_3581 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 3,991,756 to 3,992,613
_3581
Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673
_3582
Pf1N1B4_3583 - Cephalosporin hydroxylase, at 3,993,690 to 3,994,427
_3583
Pf1N1B4_3584 - O-antigen biosynthesis protein, at 3,994,479 to 3,997,415
_3584
Position (kb)
3992
3993
3994 Strain fitness (log2 ratio)
-2
-1
0
1 at 3991.646 kb on + strand at 3991.646 kb on + strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.704 kb on + strand at 3991.705 kb on - strand at 3991.911 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.997 kb on - strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.023 kb on - strand, within Pf1N1B4_3581 at 3992.251 kb on + strand, within Pf1N1B4_3581 at 3992.588 kb on + strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.618 kb on + strand at 3992.768 kb on + strand, within Pf1N1B4_3582 at 3992.769 kb on - strand, within Pf1N1B4_3582 at 3992.780 kb on + strand, within Pf1N1B4_3582 at 3993.039 kb on + strand, within Pf1N1B4_3582 at 3993.263 kb on + strand, within Pf1N1B4_3582 at 3993.599 kb on + strand at 3993.599 kb on + strand at 3993.600 kb on - strand at 3993.635 kb on + strand at 3993.636 kb on - strand at 3993.687 kb on - strand at 3993.817 kb on - strand at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.817 kb on - strand, within Pf1N1B4_3583 at 3993.823 kb on - strand at 3993.957 kb on + strand, within Pf1N1B4_3583 at 3994.064 kb on + strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.065 kb on - strand, within Pf1N1B4_3583 at 3994.200 kb on + strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.228 kb on - strand, within Pf1N1B4_3583 at 3994.329 kb on + strand, within Pf1N1B4_3583 at 3994.330 kb on - strand, within Pf1N1B4_3583 at 3994.588 kb on - strand at 3994.590 kb on + strand at 3994.590 kb on + strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.591 kb on - strand at 3994.599 kb on + strand at 3994.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,991,646 + +0.2 3,991,646 + -0.1 3,991,647 - +0.6 3,991,647 - +0.5 3,991,647 - +1.0 3,991,647 - -0.6 3,991,647 - -0.1 3,991,704 + -0.4 3,991,705 - +0.2 3,991,911 + Pf1N1B4_3581 0.18 +0.6 3,991,996 + Pf1N1B4_3581 0.28 -0.3 3,991,996 + Pf1N1B4_3581 0.28 +0.1 3,991,997 - Pf1N1B4_3581 0.28 +1.1 3,992,022 + Pf1N1B4_3581 0.31 -0.5 3,992,022 + Pf1N1B4_3581 0.31 -1.7 3,992,023 - Pf1N1B4_3581 0.31 +0.6 3,992,251 + Pf1N1B4_3581 0.58 +1.3 3,992,588 + -1.1 3,992,589 - +0.5 3,992,589 - -0.1 3,992,589 - +0.5 3,992,618 + -1.1 3,992,768 + Pf1N1B4_3582 0.15 -0.4 3,992,769 - Pf1N1B4_3582 0.15 -2.0 3,992,780 + Pf1N1B4_3582 0.17 -1.1 3,993,039 + Pf1N1B4_3582 0.41 -0.1 3,993,263 + Pf1N1B4_3582 0.62 -0.4 3,993,599 + -0.3 3,993,599 + -0.2 3,993,600 - -0.1 3,993,635 + -0.1 3,993,636 - -1.0 3,993,687 - +0.6 3,993,817 - +0.5 3,993,817 - Pf1N1B4_3583 0.17 -1.4 3,993,817 - Pf1N1B4_3583 0.17 -1.9 3,993,823 - -1.1 3,993,957 + Pf1N1B4_3583 0.36 -0.1 3,994,064 + Pf1N1B4_3583 0.51 +0.5 3,994,065 - Pf1N1B4_3583 0.51 +0.3 3,994,065 - Pf1N1B4_3583 0.51 -0.7 3,994,200 + Pf1N1B4_3583 0.69 +0.9 3,994,228 - Pf1N1B4_3583 0.73 +1.3 3,994,228 - Pf1N1B4_3583 0.73 +0.9 3,994,329 + Pf1N1B4_3583 0.87 -0.4 3,994,330 - Pf1N1B4_3583 0.87 -0.6 3,994,588 - +1.3 3,994,590 + -1.3 3,994,590 + +0.1 3,994,591 - -0.5 3,994,591 - -0.3 3,994,591 - -0.7 3,994,599 + +0.3 3,994,600 - +0.3
Or see this region's nucleotide sequence