Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3557 and Pf1N1B4_3558 are separated by 740 nucleotides Pf1N1B4_3558 and Pf1N1B4_3559 are separated by 31 nucleotides Pf1N1B4_3559 and Pf1N1B4_3560 are separated by 222 nucleotides Pf1N1B4_3560 and Pf1N1B4_3561 overlap by 4 nucleotides
Pf1N1B4_3557 - hypothetical protein, at 3,961,976 to 3,962,113
_3557
Pf1N1B4_3558 - Aromatic amino acid transport protein AroP, at 3,962,854 to 3,963,015
_3558
Pf1N1B4_3559 - Aromatic amino acid transport protein AroP, at 3,963,047 to 3,964,468
_3559
Pf1N1B4_3560 - Hypothetical protein YaeJ with similarity to translation release factor, at 3,964,691 to 3,965,104
_3560
Pf1N1B4_3561 - Xanthine transporter,putative, at 3,965,101 to 3,966,300
_3561
Position (kb)
3963
3964
3965 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3962.125 kb on + strand at 3962.125 kb on + strand at 3962.126 kb on - strand at 3962.126 kb on - strand at 3962.223 kb on + strand at 3962.224 kb on - strand at 3962.327 kb on + strand at 3962.340 kb on + strand at 3962.340 kb on + strand at 3962.340 kb on + strand at 3962.368 kb on - strand at 3962.404 kb on + strand at 3962.423 kb on - strand at 3962.431 kb on - strand at 3962.511 kb on + strand at 3962.512 kb on - strand at 3962.549 kb on + strand at 3962.550 kb on - strand at 3962.550 kb on - strand at 3962.613 kb on - strand at 3962.613 kb on - strand at 3962.613 kb on - strand at 3962.790 kb on + strand at 3962.791 kb on - strand at 3962.791 kb on - strand at 3962.791 kb on - strand at 3962.791 kb on - strand at 3962.807 kb on + strand at 3962.808 kb on - strand at 3962.808 kb on - strand at 3962.808 kb on - strand at 3962.808 kb on - strand at 3962.905 kb on - strand, within Pf1N1B4_3558 at 3963.010 kb on + strand at 3963.103 kb on + strand at 3963.302 kb on + strand, within Pf1N1B4_3559 at 3963.302 kb on + strand, within Pf1N1B4_3559 at 3963.303 kb on - strand, within Pf1N1B4_3559 at 3963.303 kb on - strand, within Pf1N1B4_3559 at 3963.353 kb on + strand, within Pf1N1B4_3559 at 3963.353 kb on + strand, within Pf1N1B4_3559 at 3963.353 kb on + strand, within Pf1N1B4_3559 at 3963.354 kb on - strand, within Pf1N1B4_3559 at 3963.354 kb on - strand at 3963.354 kb on - strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.395 kb on + strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.396 kb on - strand, within Pf1N1B4_3559 at 3963.404 kb on + strand, within Pf1N1B4_3559 at 3963.563 kb on + strand, within Pf1N1B4_3559 at 3963.563 kb on + strand, within Pf1N1B4_3559 at 3963.564 kb on - strand, within Pf1N1B4_3559 at 3963.564 kb on - strand, within Pf1N1B4_3559 at 3963.872 kb on + strand, within Pf1N1B4_3559 at 3963.872 kb on + strand, within Pf1N1B4_3559 at 3963.873 kb on - strand, within Pf1N1B4_3559 at 3963.873 kb on - strand, within Pf1N1B4_3559 at 3963.971 kb on + strand, within Pf1N1B4_3559 at 3963.972 kb on - strand, within Pf1N1B4_3559 at 3963.986 kb on + strand, within Pf1N1B4_3559 at 3963.986 kb on + strand, within Pf1N1B4_3559 at 3963.986 kb on + strand, within Pf1N1B4_3559 at 3963.987 kb on - strand, within Pf1N1B4_3559 at 3963.987 kb on - strand, within Pf1N1B4_3559 at 3963.987 kb on - strand, within Pf1N1B4_3559 at 3964.000 kb on + strand, within Pf1N1B4_3559 at 3964.127 kb on + strand, within Pf1N1B4_3559 at 3964.127 kb on + strand, within Pf1N1B4_3559 at 3964.127 kb on + strand, within Pf1N1B4_3559 at 3964.127 kb on + strand, within Pf1N1B4_3559 at 3964.128 kb on - strand, within Pf1N1B4_3559 at 3964.128 kb on - strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.292 kb on + strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.293 kb on - strand, within Pf1N1B4_3559 at 3964.364 kb on + strand at 3964.364 kb on + strand at 3964.364 kb on + strand at 3964.365 kb on - strand at 3964.466 kb on + strand at 3964.466 kb on + strand at 3964.466 kb on + strand at 3964.466 kb on + strand at 3964.467 kb on - strand at 3964.467 kb on - strand at 3964.467 kb on - strand at 3964.518 kb on + strand at 3964.519 kb on - strand at 3964.555 kb on - strand at 3964.649 kb on + strand at 3964.845 kb on - strand, within Pf1N1B4_3560 at 3964.845 kb on - strand, within Pf1N1B4_3560 at 3965.127 kb on - strand at 3965.144 kb on + strand at 3965.150 kb on + strand at 3965.150 kb on + strand at 3965.150 kb on + strand at 3965.151 kb on - strand at 3965.151 kb on - strand at 3965.151 kb on - strand at 3965.151 kb on - strand at 3965.151 kb on - strand at 3965.158 kb on + strand at 3965.158 kb on + strand at 3965.158 kb on + strand at 3965.158 kb on + strand at 3965.159 kb on - strand at 3965.159 kb on - strand at 3965.159 kb on - strand at 3965.161 kb on + strand at 3965.161 kb on + strand at 3965.162 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,962,125 + -0.7 3,962,125 + +0.5 3,962,126 - -0.1 3,962,126 - -0.1 3,962,223 + +0.9 3,962,224 - -0.3 3,962,327 + +1.0 3,962,340 + +0.9 3,962,340 + -0.1 3,962,340 + -0.4 3,962,368 - -0.3 3,962,404 + -2.1 3,962,423 - -0.4 3,962,431 - -1.4 3,962,511 + -0.1 3,962,512 - -0.7 3,962,549 + +0.3 3,962,550 - +0.5 3,962,550 - -1.8 3,962,613 - -0.4 3,962,613 - +0.1 3,962,613 - -0.1 3,962,790 + -1.1 3,962,791 - -1.7 3,962,791 - -1.1 3,962,791 - -1.9 3,962,791 - -0.9 3,962,807 + +0.3 3,962,808 - -1.1 3,962,808 - -2.6 3,962,808 - -1.2 3,962,808 - -3.4 3,962,905 - Pf1N1B4_3558 0.31 -1.7 3,963,010 + +1.7 3,963,103 + -0.4 3,963,302 + Pf1N1B4_3559 0.18 -0.1 3,963,302 + Pf1N1B4_3559 0.18 -1.7 3,963,303 - Pf1N1B4_3559 0.18 -0.3 3,963,303 - Pf1N1B4_3559 0.18 -0.7 3,963,353 + Pf1N1B4_3559 0.22 -0.6 3,963,353 + Pf1N1B4_3559 0.22 -0.8 3,963,353 + Pf1N1B4_3559 0.22 -0.5 3,963,354 - Pf1N1B4_3559 0.22 -0.9 3,963,354 - -0.7 3,963,354 - Pf1N1B4_3559 0.22 -0.7 3,963,395 + Pf1N1B4_3559 0.24 -0.7 3,963,395 + Pf1N1B4_3559 0.24 +0.9 3,963,395 + Pf1N1B4_3559 0.24 -0.9 3,963,395 + Pf1N1B4_3559 0.24 -0.9 3,963,395 + Pf1N1B4_3559 0.24 -2.9 3,963,395 + Pf1N1B4_3559 0.24 -0.5 3,963,396 - Pf1N1B4_3559 0.25 -2.1 3,963,396 - Pf1N1B4_3559 0.25 -1.4 3,963,396 - Pf1N1B4_3559 0.25 -1.1 3,963,396 - Pf1N1B4_3559 0.25 -0.5 3,963,396 - Pf1N1B4_3559 0.25 -1.6 3,963,396 - Pf1N1B4_3559 0.25 -0.1 3,963,404 + Pf1N1B4_3559 0.25 -0.5 3,963,563 + Pf1N1B4_3559 0.36 +0.6 3,963,563 + Pf1N1B4_3559 0.36 -1.6 3,963,564 - Pf1N1B4_3559 0.36 +1.9 3,963,564 - Pf1N1B4_3559 0.36 -0.6 3,963,872 + Pf1N1B4_3559 0.58 -0.7 3,963,872 + Pf1N1B4_3559 0.58 -2.0 3,963,873 - Pf1N1B4_3559 0.58 +0.9 3,963,873 - Pf1N1B4_3559 0.58 -2.1 3,963,971 + Pf1N1B4_3559 0.65 -3.1 3,963,972 - Pf1N1B4_3559 0.65 +0.5 3,963,986 + Pf1N1B4_3559 0.66 -0.9 3,963,986 + Pf1N1B4_3559 0.66 -0.3 3,963,986 + Pf1N1B4_3559 0.66 -1.4 3,963,987 - Pf1N1B4_3559 0.66 -1.5 3,963,987 - Pf1N1B4_3559 0.66 -0.1 3,963,987 - Pf1N1B4_3559 0.66 -0.9 3,964,000 + Pf1N1B4_3559 0.67 -1.5 3,964,127 + Pf1N1B4_3559 0.76 -1.1 3,964,127 + Pf1N1B4_3559 0.76 -2.0 3,964,127 + Pf1N1B4_3559 0.76 -2.5 3,964,127 + Pf1N1B4_3559 0.76 -0.7 3,964,128 - Pf1N1B4_3559 0.76 +0.1 3,964,128 - Pf1N1B4_3559 0.76 -0.5 3,964,292 + Pf1N1B4_3559 0.88 -1.5 3,964,292 + Pf1N1B4_3559 0.88 +1.5 3,964,292 + Pf1N1B4_3559 0.88 +0.6 3,964,292 + Pf1N1B4_3559 0.88 -2.1 3,964,292 + Pf1N1B4_3559 0.88 -0.7 3,964,292 + Pf1N1B4_3559 0.88 -1.8 3,964,292 + Pf1N1B4_3559 0.88 -0.7 3,964,292 + Pf1N1B4_3559 0.88 -1.1 3,964,292 + Pf1N1B4_3559 0.88 -1.6 3,964,292 + Pf1N1B4_3559 0.88 -0.4 3,964,292 + Pf1N1B4_3559 0.88 -2.7 3,964,292 + Pf1N1B4_3559 0.88 -1.2 3,964,292 + Pf1N1B4_3559 0.88 -1.3 3,964,292 + Pf1N1B4_3559 0.88 -1.9 3,964,292 + Pf1N1B4_3559 0.88 -0.7 3,964,292 + Pf1N1B4_3559 0.88 -1.9 3,964,293 - Pf1N1B4_3559 0.88 +0.1 3,964,293 - Pf1N1B4_3559 0.88 -1.4 3,964,293 - Pf1N1B4_3559 0.88 -1.9 3,964,293 - Pf1N1B4_3559 0.88 -0.1 3,964,293 - Pf1N1B4_3559 0.88 -1.4 3,964,293 - Pf1N1B4_3559 0.88 -1.6 3,964,293 - Pf1N1B4_3559 0.88 -1.5 3,964,293 - Pf1N1B4_3559 0.88 +0.3 3,964,293 - Pf1N1B4_3559 0.88 -0.1 3,964,293 - Pf1N1B4_3559 0.88 -1.8 3,964,293 - Pf1N1B4_3559 0.88 -1.4 3,964,293 - Pf1N1B4_3559 0.88 -1.6 3,964,293 - Pf1N1B4_3559 0.88 -1.3 3,964,293 - Pf1N1B4_3559 0.88 -1.0 3,964,293 - Pf1N1B4_3559 0.88 -0.4 3,964,293 - Pf1N1B4_3559 0.88 +0.4 3,964,293 - Pf1N1B4_3559 0.88 -2.1 3,964,293 - Pf1N1B4_3559 0.88 +0.4 3,964,293 - Pf1N1B4_3559 0.88 -1.4 3,964,364 + -1.1 3,964,364 + -3.7 3,964,364 + +1.2 3,964,365 - -0.7 3,964,466 + +2.9 3,964,466 + -0.3 3,964,466 + -0.8 3,964,466 + +0.6 3,964,467 - -1.1 3,964,467 - +0.9 3,964,467 - -0.4 3,964,518 + +0.9 3,964,519 - +0.6 3,964,555 - -2.6 3,964,649 + +0.9 3,964,845 - Pf1N1B4_3560 0.37 -0.3 3,964,845 - Pf1N1B4_3560 0.37 -0.3 3,965,127 - +0.6 3,965,144 + +0.7 3,965,150 + +0.2 3,965,150 + +0.3 3,965,150 + +1.5 3,965,151 - +0.4 3,965,151 - -1.4 3,965,151 - -1.1 3,965,151 - -2.8 3,965,151 - -0.7 3,965,158 + +0.5 3,965,158 + -0.7 3,965,158 + -0.1 3,965,158 + -0.5 3,965,159 - -0.7 3,965,159 - +1.5 3,965,159 - +0.3 3,965,161 + -0.6 3,965,161 + +0.2 3,965,162 - -1.1
Or see this region's nucleotide sequence