Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3145 and Pf1N1B4_3146 are separated by 488 nucleotides Pf1N1B4_3146 and Pf1N1B4_3147 are separated by 0 nucleotides Pf1N1B4_3147 and Pf1N1B4_3148 are separated by 189 nucleotides
Pf1N1B4_3145 - Decarboxylase family protein, at 3,527,322 to 3,528,440
_3145
Pf1N1B4_3146 - FIG00957354: hypothetical protein, at 3,528,929 to 3,529,123
_3146
Pf1N1B4_3147 - Putative NADPH-quinone reductase (modulator of drug activity B), at 3,529,124 to 3,529,549
_3147
Pf1N1B4_3148 - FIG074102: hypothetical protein, at 3,529,739 to 3,530,482
_3148
Position (kb)
3529
3530 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.136 kb on + strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.137 kb on - strand, within Pf1N1B4_3145 at 3528.150 kb on + strand, within Pf1N1B4_3145 at 3528.586 kb on + strand at 3528.587 kb on - strand at 3528.617 kb on + strand at 3528.618 kb on - strand at 3528.618 kb on - strand at 3528.679 kb on + strand at 3528.679 kb on + strand at 3528.743 kb on + strand at 3528.744 kb on - strand at 3528.744 kb on - strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.810 kb on + strand at 3528.813 kb on + strand at 3528.813 kb on + strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.818 kb on + strand at 3528.819 kb on - strand at 3528.819 kb on - strand at 3528.965 kb on + strand, within Pf1N1B4_3146 at 3529.058 kb on + strand, within Pf1N1B4_3146 at 3529.126 kb on - strand at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.436 kb on + strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.538 kb on - strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.619 kb on - strand at 3529.620 kb on + strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.681 kb on + strand at 3529.681 kb on + strand at 3529.869 kb on + strand, within Pf1N1B4_3148 at 3529.870 kb on - strand, within Pf1N1B4_3148 at 3529.927 kb on + strand, within Pf1N1B4_3148 at 3530.063 kb on + strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.084 kb on + strand at 3530.105 kb on - strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.361 kb on - strand, within Pf1N1B4_3148 at 3530.384 kb on + strand, within Pf1N1B4_3148 at 3530.385 kb on - strand, within Pf1N1B4_3148 at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.493 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,528,136 + Pf1N1B4_3145 0.73 -1.0 3,528,136 + Pf1N1B4_3145 0.73 -0.2 3,528,137 - Pf1N1B4_3145 0.73 +1.1 3,528,137 - Pf1N1B4_3145 0.73 -0.5 3,528,150 + Pf1N1B4_3145 0.74 +1.7 3,528,586 + -1.1 3,528,587 - -1.5 3,528,617 + +1.7 3,528,618 - -1.5 3,528,618 - +0.8 3,528,679 + +0.3 3,528,679 + +0.1 3,528,743 + -0.9 3,528,744 - -0.3 3,528,744 - +1.5 3,528,791 + -0.5 3,528,791 + -0.9 3,528,791 + -0.4 3,528,792 - +0.6 3,528,792 - +1.1 3,528,792 - +0.1 3,528,792 - +0.8 3,528,810 + -1.1 3,528,813 + -0.5 3,528,813 + -1.1 3,528,814 - -0.5 3,528,814 - +2.5 3,528,814 - +0.2 3,528,814 - +0.1 3,528,814 - +0.1 3,528,818 + -0.4 3,528,819 - +0.1 3,528,819 - +1.7 3,528,965 + Pf1N1B4_3146 0.18 +1.1 3,529,058 + Pf1N1B4_3146 0.66 +0.9 3,529,126 - +0.3 3,529,293 + Pf1N1B4_3147 0.40 +0.7 3,529,293 + Pf1N1B4_3147 0.40 -0.1 3,529,294 - Pf1N1B4_3147 0.40 +1.5 3,529,294 - Pf1N1B4_3147 0.40 +0.1 3,529,294 - Pf1N1B4_3147 0.40 +1.2 3,529,378 + Pf1N1B4_3147 0.60 -1.3 3,529,378 + Pf1N1B4_3147 0.60 +0.3 3,529,378 + Pf1N1B4_3147 0.60 +0.7 3,529,378 + Pf1N1B4_3147 0.60 -0.5 3,529,378 + Pf1N1B4_3147 0.60 +0.8 3,529,378 + Pf1N1B4_3147 0.60 +2.1 3,529,378 + Pf1N1B4_3147 0.60 -0.7 3,529,379 - Pf1N1B4_3147 0.60 -0.3 3,529,379 - Pf1N1B4_3147 0.60 +0.9 3,529,379 - Pf1N1B4_3147 0.60 -0.8 3,529,379 - Pf1N1B4_3147 0.60 +0.1 3,529,379 - Pf1N1B4_3147 0.60 -0.1 3,529,379 - Pf1N1B4_3147 0.60 +1.1 3,529,379 - Pf1N1B4_3147 0.60 +0.9 3,529,379 - Pf1N1B4_3147 0.60 +0.5 3,529,379 - Pf1N1B4_3147 0.60 -0.3 3,529,380 + Pf1N1B4_3147 0.60 +0.9 3,529,380 + Pf1N1B4_3147 0.60 +0.5 3,529,380 + Pf1N1B4_3147 0.60 +0.3 3,529,381 - Pf1N1B4_3147 0.60 +0.2 3,529,381 - Pf1N1B4_3147 0.60 +3.1 3,529,381 - Pf1N1B4_3147 0.60 +0.6 3,529,381 - Pf1N1B4_3147 0.60 +1.9 3,529,436 + Pf1N1B4_3147 0.73 +1.2 3,529,437 - Pf1N1B4_3147 0.73 +0.8 3,529,437 - Pf1N1B4_3147 0.73 +0.7 3,529,437 - Pf1N1B4_3147 0.73 -0.6 3,529,437 - Pf1N1B4_3147 0.73 +0.9 3,529,437 - Pf1N1B4_3147 0.73 +0.4 3,529,538 - -0.3 3,529,618 + +0.9 3,529,618 + +0.5 3,529,618 + -1.9 3,529,618 + +0.7 3,529,618 + +1.1 3,529,618 + +0.1 3,529,619 - +1.7 3,529,620 + +1.6 3,529,621 - -0.5 3,529,621 - +1.4 3,529,621 - +0.7 3,529,621 - +0.3 3,529,681 + -0.3 3,529,681 + +0.7 3,529,869 + Pf1N1B4_3148 0.17 +1.7 3,529,870 - Pf1N1B4_3148 0.18 +0.9 3,529,927 + Pf1N1B4_3148 0.25 +0.5 3,530,063 + Pf1N1B4_3148 0.44 +0.8 3,530,064 - Pf1N1B4_3148 0.44 +0.7 3,530,064 - Pf1N1B4_3148 0.44 +0.7 3,530,084 + -0.5 3,530,105 - Pf1N1B4_3148 0.49 +0.1 3,530,360 + Pf1N1B4_3148 0.83 +1.3 3,530,360 + Pf1N1B4_3148 0.83 +1.4 3,530,360 + Pf1N1B4_3148 0.83 +0.1 3,530,361 - Pf1N1B4_3148 0.84 -0.4 3,530,384 + Pf1N1B4_3148 0.87 -0.3 3,530,385 - Pf1N1B4_3148 0.87 +0.5 3,530,454 + -0.2 3,530,454 + -2.5 3,530,454 + -1.5 3,530,454 + -0.9 3,530,454 + -0.3 3,530,493 + +1.1
Or see this region's nucleotide sequence