Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2887 and Pf1N1B4_2888 overlap by 4 nucleotides Pf1N1B4_2888 and Pf1N1B4_2889 overlap by 11 nucleotides Pf1N1B4_2889 and Pf1N1B4_2890 are separated by 101 nucleotides Pf1N1B4_2890 and Pf1N1B4_2891 overlap by 8 nucleotides
Pf1N1B4_2887 - Chromosome segregation ATPases, at 3,240,173 to 3,243,013
_2887
Pf1N1B4_2888 - FIG00961017: hypothetical protein, at 3,243,010 to 3,243,711
_2888
Pf1N1B4_2889 - FIG00954491: hypothetical protein, at 3,243,701 to 3,245,044
_2889
Pf1N1B4_2890 - Periplasmic protein TonB, links inner and outer membranes, at 3,245,146 to 3,245,925
_2890
Pf1N1B4_2891 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 3,245,918 to 3,246,370
_2891
Position (kb)
3243
3244
3245
3246 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3242.738 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.778 kb on + strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.779 kb on - strand at 3242.799 kb on + strand at 3242.800 kb on - strand at 3242.800 kb on - strand at 3242.826 kb on + strand at 3242.826 kb on + strand at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.252 kb on - strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.359 kb on + strand, within Pf1N1B4_2888 at 3243.497 kb on - strand, within Pf1N1B4_2888 at 3243.497 kb on - strand at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.528 kb on - strand, within Pf1N1B4_2888 at 3243.530 kb on + strand, within Pf1N1B4_2888 at 3243.531 kb on - strand, within Pf1N1B4_2888 at 3243.582 kb on + strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.583 kb on - strand, within Pf1N1B4_2888 at 3243.697 kb on + strand at 3243.698 kb on - strand at 3243.698 kb on - strand at 3244.057 kb on - strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.062 kb on + strand, within Pf1N1B4_2889 at 3244.063 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.441 kb on - strand, within Pf1N1B4_2889 at 3244.471 kb on - strand, within Pf1N1B4_2889 at 3244.644 kb on + strand, within Pf1N1B4_2889 at 3244.645 kb on - strand, within Pf1N1B4_2889 at 3244.674 kb on + strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889 at 3244.675 kb on - strand, within Pf1N1B4_2889 at 3244.722 kb on + strand, within Pf1N1B4_2889 at 3244.723 kb on - strand, within Pf1N1B4_2889 at 3245.004 kb on - strand at 3245.018 kb on + strand at 3245.018 kb on + strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.069 kb on + strand at 3245.070 kb on - strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.931 kb on + strand at 3245.970 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand at 3246.041 kb on + strand, within Pf1N1B4_2891 at 3246.042 kb on - strand, within Pf1N1B4_2891
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 3,242,738 + +0.3 3,242,778 + +0.2 3,242,778 + +2.2 3,242,778 + -0.1 3,242,778 + -1.2 3,242,778 + +0.3 3,242,779 - +1.6 3,242,779 - +0.2 3,242,779 - -0.8 3,242,779 - +1.2 3,242,779 - -0.8 3,242,779 - -0.4 3,242,779 - +0.9 3,242,779 - +0.2 3,242,779 - +0.2 3,242,779 - -0.2 3,242,799 + +1.2 3,242,800 - +1.0 3,242,800 - +1.7 3,242,826 + -1.1 3,242,826 + +1.2 3,243,252 - Pf1N1B4_2888 0.34 -0.3 3,243,252 - Pf1N1B4_2888 0.34 -0.3 3,243,359 + Pf1N1B4_2888 0.50 +1.5 3,243,359 + Pf1N1B4_2888 0.50 -1.4 3,243,497 - Pf1N1B4_2888 0.69 -1.2 3,243,497 - +1.7 3,243,528 - Pf1N1B4_2888 0.74 -1.3 3,243,528 - Pf1N1B4_2888 0.74 -0.3 3,243,528 - Pf1N1B4_2888 0.74 +0.7 3,243,530 + Pf1N1B4_2888 0.74 +0.3 3,243,531 - Pf1N1B4_2888 0.74 +0.6 3,243,582 + Pf1N1B4_2888 0.81 -0.5 3,243,583 - Pf1N1B4_2888 0.82 -0.7 3,243,583 - Pf1N1B4_2888 0.82 -1.2 3,243,697 + +0.2 3,243,698 - -0.4 3,243,698 - +1.4 3,244,057 - Pf1N1B4_2889 0.26 -1.3 3,244,062 + Pf1N1B4_2889 0.27 -1.7 3,244,062 + Pf1N1B4_2889 0.27 -1.8 3,244,062 + Pf1N1B4_2889 0.27 -1.8 3,244,063 - Pf1N1B4_2889 0.27 -1.7 3,244,441 - Pf1N1B4_2889 0.55 -0.2 3,244,441 - Pf1N1B4_2889 0.55 +1.7 3,244,471 - Pf1N1B4_2889 0.57 -0.4 3,244,644 + Pf1N1B4_2889 0.70 -1.0 3,244,645 - Pf1N1B4_2889 0.70 -0.7 3,244,674 + Pf1N1B4_2889 0.72 +0.2 3,244,675 - Pf1N1B4_2889 0.72 +0.2 3,244,675 - Pf1N1B4_2889 0.72 +0.6 3,244,722 + Pf1N1B4_2889 0.76 -1.7 3,244,723 - Pf1N1B4_2889 0.76 -2.0 3,245,004 - +0.7 3,245,018 + -0.7 3,245,018 + +0.5 3,245,019 - +1.9 3,245,019 - +1.0 3,245,019 - -1.2 3,245,069 + +1.2 3,245,070 - +1.2 3,245,122 + +1.0 3,245,122 + +0.3 3,245,122 + +0.8 3,245,931 + +0.4 3,245,970 + Pf1N1B4_2891 0.11 -1.4 3,245,993 + Pf1N1B4_2891 0.17 +0.6 3,245,993 + +1.2 3,246,041 + Pf1N1B4_2891 0.27 -2.0 3,246,042 - Pf1N1B4_2891 0.27 -3.2
Or see this region's nucleotide sequence