Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2617 and Pf1N1B4_2618 are separated by 15 nucleotides Pf1N1B4_2618 and Pf1N1B4_2619 overlap by 14 nucleotides Pf1N1B4_2619 and Pf1N1B4_2620 are separated by 69 nucleotides Pf1N1B4_2620 and Pf1N1B4_2621 are separated by 156 nucleotides
Pf1N1B4_2617 - Membrane protein with DUF350 domain, at 2,958,287 to 2,958,715
_2617
Pf1N1B4_2618 - FIG00955711: hypothetical protein, at 2,958,731 to 2,959,405
_2618
Pf1N1B4_2619 - Putative potassium channel protein, at 2,959,392 to 2,960,426
_2619
Pf1N1B4_2620 - GlpG protein (membrane protein of glp regulon), at 2,960,496 to 2,961,059
_2620
Pf1N1B4_2621 - PspA/IM30 family protein, at 2,961,216 to 2,961,914
_2621
Position (kb)
2959
2960
2961 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2958.404 kb on - strand, within Pf1N1B4_2617 at 2958.440 kb on + strand at 2958.441 kb on - strand at 2958.492 kb on + strand, within Pf1N1B4_2617 at 2958.612 kb on + strand, within Pf1N1B4_2617 at 2958.612 kb on + strand, within Pf1N1B4_2617 at 2958.612 kb on + strand, within Pf1N1B4_2617 at 2958.612 kb on + strand at 2958.613 kb on - strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.657 kb on + strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.658 kb on - strand, within Pf1N1B4_2617 at 2958.728 kb on + strand at 2958.729 kb on - strand at 2958.729 kb on - strand at 2958.732 kb on + strand at 2958.733 kb on - strand at 2958.756 kb on + strand at 2958.756 kb on + strand at 2958.756 kb on + strand at 2958.756 kb on + strand at 2958.757 kb on - strand at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.864 kb on + strand, within Pf1N1B4_2618 at 2958.891 kb on + strand, within Pf1N1B4_2618 at 2958.891 kb on + strand, within Pf1N1B4_2618 at 2958.891 kb on + strand, within Pf1N1B4_2618 at 2958.892 kb on - strand, within Pf1N1B4_2618 at 2958.972 kb on + strand, within Pf1N1B4_2618 at 2958.972 kb on + strand, within Pf1N1B4_2618 at 2958.973 kb on - strand, within Pf1N1B4_2618 at 2958.973 kb on - strand at 2958.993 kb on + strand at 2958.993 kb on + strand, within Pf1N1B4_2618 at 2958.993 kb on + strand, within Pf1N1B4_2618 at 2958.994 kb on - strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.065 kb on + strand, within Pf1N1B4_2618 at 2959.066 kb on - strand, within Pf1N1B4_2618 at 2959.066 kb on - strand, within Pf1N1B4_2618 at 2959.066 kb on - strand, within Pf1N1B4_2618 at 2959.158 kb on + strand, within Pf1N1B4_2618 at 2959.158 kb on + strand, within Pf1N1B4_2618 at 2959.158 kb on + strand, within Pf1N1B4_2618 at 2959.159 kb on - strand, within Pf1N1B4_2618 at 2959.159 kb on - strand, within Pf1N1B4_2618 at 2959.161 kb on + strand, within Pf1N1B4_2618 at 2959.161 kb on + strand, within Pf1N1B4_2618 at 2959.161 kb on + strand, within Pf1N1B4_2618 at 2959.394 kb on - strand at 2959.438 kb on + strand at 2959.438 kb on + strand at 2959.438 kb on + strand at 2959.439 kb on - strand at 2959.439 kb on - strand at 2959.439 kb on - strand at 2959.603 kb on + strand, within Pf1N1B4_2619 at 2959.677 kb on + strand, within Pf1N1B4_2619 at 2959.677 kb on + strand, within Pf1N1B4_2619 at 2959.920 kb on - strand, within Pf1N1B4_2619 at 2960.010 kb on + strand, within Pf1N1B4_2619 at 2960.055 kb on + strand, within Pf1N1B4_2619 at 2960.056 kb on - strand, within Pf1N1B4_2619 at 2960.112 kb on + strand, within Pf1N1B4_2619 at 2960.112 kb on + strand, within Pf1N1B4_2619 at 2960.112 kb on + strand at 2960.112 kb on + strand, within Pf1N1B4_2619 at 2960.112 kb on + strand, within Pf1N1B4_2619 at 2960.113 kb on - strand, within Pf1N1B4_2619 at 2960.113 kb on - strand, within Pf1N1B4_2619 at 2960.260 kb on + strand, within Pf1N1B4_2619 at 2960.260 kb on + strand, within Pf1N1B4_2619 at 2960.261 kb on - strand at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.261 kb on - strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.266 kb on + strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.267 kb on - strand, within Pf1N1B4_2619 at 2960.270 kb on + strand, within Pf1N1B4_2619 at 2960.271 kb on - strand, within Pf1N1B4_2619 at 2960.323 kb on + strand at 2960.323 kb on + strand at 2960.323 kb on + strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.324 kb on - strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.334 kb on + strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.335 kb on - strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.340 kb on + strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.341 kb on - strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.398 kb on + strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.399 kb on - strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.409 kb on + strand at 2960.410 kb on - strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.427 kb on + strand at 2960.428 kb on - strand at 2960.428 kb on - strand at 2960.428 kb on - strand at 2960.463 kb on + strand at 2960.510 kb on + strand at 2960.510 kb on + strand at 2960.528 kb on + strand at 2960.532 kb on + strand at 2960.533 kb on - strand at 2960.538 kb on + strand at 2960.552 kb on + strand at 2960.552 kb on + strand at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.576 kb on + strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.577 kb on - strand, within Pf1N1B4_2620 at 2960.632 kb on + strand, within Pf1N1B4_2620 at 2960.632 kb on + strand, within Pf1N1B4_2620 at 2960.633 kb on - strand, within Pf1N1B4_2620 at 2960.633 kb on - strand, within Pf1N1B4_2620 at 2960.705 kb on - strand, within Pf1N1B4_2620 at 2960.705 kb on - strand, within Pf1N1B4_2620 at 2960.792 kb on + strand, within Pf1N1B4_2620 at 2960.793 kb on - strand, within Pf1N1B4_2620 at 2960.798 kb on + strand, within Pf1N1B4_2620 at 2960.799 kb on - strand, within Pf1N1B4_2620 at 2960.846 kb on + strand, within Pf1N1B4_2620 at 2960.966 kb on + strand, within Pf1N1B4_2620 at 2960.967 kb on - strand, within Pf1N1B4_2620 at 2960.975 kb on - strand, within Pf1N1B4_2620 at 2961.084 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 2,958,404 - Pf1N1B4_2617 0.27 +0.9 2,958,440 + +1.9 2,958,441 - +0.3 2,958,492 + Pf1N1B4_2617 0.48 +0.3 2,958,612 + Pf1N1B4_2617 0.76 +0.1 2,958,612 + Pf1N1B4_2617 0.76 -0.7 2,958,612 + Pf1N1B4_2617 0.76 -2.3 2,958,612 + +0.3 2,958,613 - Pf1N1B4_2617 0.76 +0.9 2,958,657 + Pf1N1B4_2617 0.86 +0.6 2,958,657 + Pf1N1B4_2617 0.86 -1.3 2,958,657 + Pf1N1B4_2617 0.86 +0.5 2,958,657 + Pf1N1B4_2617 0.86 -1.3 2,958,657 + Pf1N1B4_2617 0.86 +0.6 2,958,657 + Pf1N1B4_2617 0.86 -0.3 2,958,658 - Pf1N1B4_2617 0.86 -1.9 2,958,658 - Pf1N1B4_2617 0.86 +2.5 2,958,658 - Pf1N1B4_2617 0.86 +0.5 2,958,658 - Pf1N1B4_2617 0.86 +0.9 2,958,658 - Pf1N1B4_2617 0.86 -1.0 2,958,658 - Pf1N1B4_2617 0.86 +0.4 2,958,728 + -0.5 2,958,729 - -1.3 2,958,729 - -0.8 2,958,732 + +1.0 2,958,733 - -1.0 2,958,756 + +0.3 2,958,756 + -1.0 2,958,756 + -0.2 2,958,756 + -1.3 2,958,757 - -2.5 2,958,864 + Pf1N1B4_2618 0.20 +0.2 2,958,864 + Pf1N1B4_2618 0.20 -1.3 2,958,864 + Pf1N1B4_2618 0.20 -0.1 2,958,864 + Pf1N1B4_2618 0.20 -1.2 2,958,864 + Pf1N1B4_2618 0.20 -1.0 2,958,864 + Pf1N1B4_2618 0.20 -0.6 2,958,891 + Pf1N1B4_2618 0.24 +0.4 2,958,891 + Pf1N1B4_2618 0.24 -1.3 2,958,891 + Pf1N1B4_2618 0.24 -1.3 2,958,892 - Pf1N1B4_2618 0.24 +0.7 2,958,972 + Pf1N1B4_2618 0.36 -0.9 2,958,972 + Pf1N1B4_2618 0.36 +0.4 2,958,973 - Pf1N1B4_2618 0.36 -2.0 2,958,973 - +1.3 2,958,993 + -0.3 2,958,993 + Pf1N1B4_2618 0.39 +0.5 2,958,993 + Pf1N1B4_2618 0.39 -0.3 2,958,994 - Pf1N1B4_2618 0.39 -0.3 2,959,065 + Pf1N1B4_2618 0.49 -0.3 2,959,065 + Pf1N1B4_2618 0.49 +0.7 2,959,065 + Pf1N1B4_2618 0.49 +0.1 2,959,065 + Pf1N1B4_2618 0.49 +1.3 2,959,065 + Pf1N1B4_2618 0.49 +0.1 2,959,065 + +1.3 2,959,065 + Pf1N1B4_2618 0.49 +0.9 2,959,065 + Pf1N1B4_2618 0.49 +0.2 2,959,065 + Pf1N1B4_2618 0.49 +0.7 2,959,066 - Pf1N1B4_2618 0.50 -1.0 2,959,066 - Pf1N1B4_2618 0.50 -1.3 2,959,066 - Pf1N1B4_2618 0.50 -1.0 2,959,158 + Pf1N1B4_2618 0.63 -0.7 2,959,158 + Pf1N1B4_2618 0.63 -0.3 2,959,158 + Pf1N1B4_2618 0.63 -0.5 2,959,159 - Pf1N1B4_2618 0.63 -1.3 2,959,159 - Pf1N1B4_2618 0.63 +0.3 2,959,161 + Pf1N1B4_2618 0.64 +1.7 2,959,161 + Pf1N1B4_2618 0.64 -0.7 2,959,161 + Pf1N1B4_2618 0.64 +1.7 2,959,394 - -1.9 2,959,438 + +1.5 2,959,438 + +0.1 2,959,438 + +1.3 2,959,439 - -1.0 2,959,439 - -1.0 2,959,439 - -1.0 2,959,603 + Pf1N1B4_2619 0.20 +0.3 2,959,677 + Pf1N1B4_2619 0.28 -1.1 2,959,677 + Pf1N1B4_2619 0.28 -1.2 2,959,920 - Pf1N1B4_2619 0.51 +0.5 2,960,010 + Pf1N1B4_2619 0.60 -0.9 2,960,055 + Pf1N1B4_2619 0.64 -1.3 2,960,056 - Pf1N1B4_2619 0.64 +0.1 2,960,112 + Pf1N1B4_2619 0.70 -0.7 2,960,112 + Pf1N1B4_2619 0.70 +0.9 2,960,112 + +0.3 2,960,112 + Pf1N1B4_2619 0.70 -1.5 2,960,112 + Pf1N1B4_2619 0.70 -1.3 2,960,113 - Pf1N1B4_2619 0.70 +0.3 2,960,113 - Pf1N1B4_2619 0.70 -0.7 2,960,260 + Pf1N1B4_2619 0.84 -0.3 2,960,260 + Pf1N1B4_2619 0.84 -0.2 2,960,261 - +0.5 2,960,261 - Pf1N1B4_2619 0.84 -1.0 2,960,261 - Pf1N1B4_2619 0.84 -0.7 2,960,261 - Pf1N1B4_2619 0.84 -1.9 2,960,261 - Pf1N1B4_2619 0.84 -1.0 2,960,266 + Pf1N1B4_2619 0.84 -0.9 2,960,266 + Pf1N1B4_2619 0.84 +0.9 2,960,266 + Pf1N1B4_2619 0.84 +0.2 2,960,266 + Pf1N1B4_2619 0.84 -0.5 2,960,267 - Pf1N1B4_2619 0.85 -1.7 2,960,267 - Pf1N1B4_2619 0.85 -1.5 2,960,267 - Pf1N1B4_2619 0.85 -1.0 2,960,267 - Pf1N1B4_2619 0.85 -1.7 2,960,267 - Pf1N1B4_2619 0.85 -2.5 2,960,267 - Pf1N1B4_2619 0.85 -0.7 2,960,270 + Pf1N1B4_2619 0.85 +0.6 2,960,271 - Pf1N1B4_2619 0.85 -1.0 2,960,323 + -0.0 2,960,323 + -1.0 2,960,323 + -2.4 2,960,324 - +1.0 2,960,324 - +0.7 2,960,324 - -1.7 2,960,324 - -1.5 2,960,334 + +0.3 2,960,334 + -0.3 2,960,334 + +0.7 2,960,334 + -1.0 2,960,334 + -0.7 2,960,334 + -0.2 2,960,334 + -1.0 2,960,334 + -1.0 2,960,334 + +1.7 2,960,334 + -1.0 2,960,334 + -2.0 2,960,335 - -2.4 2,960,335 - -0.9 2,960,335 - +0.3 2,960,335 - +0.3 2,960,335 - +1.3 2,960,335 - -0.7 2,960,335 - +1.3 2,960,340 + +0.3 2,960,340 + -0.7 2,960,340 + -0.7 2,960,340 + +0.1 2,960,340 + -0.5 2,960,341 - +0.1 2,960,341 - +0.9 2,960,341 - +0.1 2,960,341 - -0.9 2,960,398 + -0.3 2,960,398 + +0.5 2,960,398 + +0.3 2,960,398 + +0.1 2,960,399 - +1.3 2,960,399 - -0.7 2,960,399 - -0.4 2,960,399 - +1.6 2,960,409 + -0.1 2,960,409 + +2.1 2,960,409 + -0.9 2,960,409 + +1.0 2,960,410 - +1.3 2,960,427 + +1.3 2,960,427 + -0.3 2,960,427 + -0.8 2,960,427 + -0.8 2,960,427 + +0.3 2,960,427 + -0.9 2,960,427 + +0.5 2,960,427 + +0.9 2,960,427 + -1.2 2,960,427 + -1.0 2,960,428 - -0.0 2,960,428 - +0.5 2,960,428 - +2.3 2,960,463 + -0.7 2,960,510 + -0.5 2,960,510 + +0.9 2,960,528 + -1.0 2,960,532 + -0.0 2,960,533 - -0.5 2,960,538 + +1.9 2,960,552 + +0.1 2,960,552 + +0.1 2,960,576 + Pf1N1B4_2620 0.14 -0.3 2,960,576 + Pf1N1B4_2620 0.14 -0.7 2,960,576 + Pf1N1B4_2620 0.14 +0.5 2,960,576 + Pf1N1B4_2620 0.14 -0.8 2,960,576 + +0.7 2,960,576 + Pf1N1B4_2620 0.14 +0.9 2,960,576 + Pf1N1B4_2620 0.14 +0.1 2,960,577 - Pf1N1B4_2620 0.14 -0.7 2,960,577 - Pf1N1B4_2620 0.14 -1.8 2,960,577 - Pf1N1B4_2620 0.14 -0.8 2,960,577 - Pf1N1B4_2620 0.14 -0.5 2,960,632 + Pf1N1B4_2620 0.24 +0.1 2,960,632 + Pf1N1B4_2620 0.24 +0.4 2,960,633 - Pf1N1B4_2620 0.24 -1.7 2,960,633 - Pf1N1B4_2620 0.24 -0.3 2,960,705 - Pf1N1B4_2620 0.37 +0.3 2,960,705 - Pf1N1B4_2620 0.37 +1.3 2,960,792 + Pf1N1B4_2620 0.52 -1.3 2,960,793 - Pf1N1B4_2620 0.53 -1.7 2,960,798 + Pf1N1B4_2620 0.54 -0.3 2,960,799 - Pf1N1B4_2620 0.54 -1.6 2,960,846 + Pf1N1B4_2620 0.62 -1.3 2,960,966 + Pf1N1B4_2620 0.83 +0.5 2,960,967 - Pf1N1B4_2620 0.84 +0.7 2,960,975 - Pf1N1B4_2620 0.85 +0.3 2,961,084 + -0.0
Or see this region's nucleotide sequence