Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2392 and Pf1N1B4_2393 are separated by 42 nucleotides Pf1N1B4_2393 and Pf1N1B4_2394 are separated by 90 nucleotides Pf1N1B4_2394 and Pf1N1B4_2395 are separated by 293 nucleotides
Pf1N1B4_2392 - Cytochrome c556, at 2,710,020 to 2,710,469
_2392
Pf1N1B4_2393 - Protein yqjC precursor, at 2,710,512 to 2,710,907
_2393
Pf1N1B4_2394 - DNA ligase (EC 6.5.1.2), at 2,710,998 to 2,712,677
_2394
Pf1N1B4_2395 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 2,712,971 to 2,714,161
_2395
Position (kb)
2710
2711
2712
2713 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2710.028 kb on + strand at 2710.029 kb on - strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.045 kb on + strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.046 kb on - strand at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.175 kb on + strand, within Pf1N1B4_2392 at 2710.176 kb on - strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.386 kb on + strand, within Pf1N1B4_2392 at 2710.387 kb on - strand, within Pf1N1B4_2392 at 2710.387 kb on - strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.513 kb on + strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.514 kb on - strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.517 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.907 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2710.934 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.025 kb on + strand at 2711.026 kb on - strand at 2711.285 kb on + strand, within Pf1N1B4_2394 at 2711.285 kb on + strand, within Pf1N1B4_2394 at 2711.285 kb on + strand, within Pf1N1B4_2394 at 2711.493 kb on + strand, within Pf1N1B4_2394 at 2711.493 kb on + strand, within Pf1N1B4_2394 at 2711.494 kb on - strand, within Pf1N1B4_2394 at 2711.494 kb on - strand, within Pf1N1B4_2394 at 2711.594 kb on + strand, within Pf1N1B4_2394 at 2711.829 kb on + strand, within Pf1N1B4_2394 at 2711.830 kb on - strand, within Pf1N1B4_2394 at 2711.920 kb on + strand, within Pf1N1B4_2394 at 2711.921 kb on - strand, within Pf1N1B4_2394 at 2711.921 kb on - strand, within Pf1N1B4_2394 at 2711.930 kb on + strand, within Pf1N1B4_2394 at 2711.931 kb on - strand, within Pf1N1B4_2394 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.105 kb on + strand, within Pf1N1B4_2394 at 2712.106 kb on - strand, within Pf1N1B4_2394 at 2712.127 kb on + strand, within Pf1N1B4_2394 at 2712.128 kb on - strand, within Pf1N1B4_2394 at 2712.128 kb on - strand, within Pf1N1B4_2394 at 2712.268 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.487 kb on + strand, within Pf1N1B4_2394 at 2712.488 kb on - strand, within Pf1N1B4_2394 at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.526 kb on + strand at 2712.527 kb on - strand at 2712.713 kb on + strand at 2712.713 kb on + strand at 2712.713 kb on + strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.714 kb on - strand at 2712.832 kb on - strand at 2712.972 kb on + strand at 2712.973 kb on - strand at 2712.973 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 2,710,028 + +0.0 2,710,029 - +0.1 2,710,045 + -0.8 2,710,045 + -0.8 2,710,045 + -0.0 2,710,045 + -0.9 2,710,045 + -0.2 2,710,045 + -1.0 2,710,045 + -0.2 2,710,045 + +0.3 2,710,045 + -1.2 2,710,045 + +0.8 2,710,045 + +1.3 2,710,045 + +0.3 2,710,045 + -1.3 2,710,046 - -0.8 2,710,046 - +1.0 2,710,046 - +0.4 2,710,046 - -1.0 2,710,175 + Pf1N1B4_2392 0.34 +1.7 2,710,175 + Pf1N1B4_2392 0.34 -0.3 2,710,175 + Pf1N1B4_2392 0.34 -1.3 2,710,176 - Pf1N1B4_2392 0.35 -1.0 2,710,386 + Pf1N1B4_2392 0.81 +1.1 2,710,386 + Pf1N1B4_2392 0.81 +1.2 2,710,387 - Pf1N1B4_2392 0.82 +0.3 2,710,387 - +1.3 2,710,513 + +2.1 2,710,513 + +0.6 2,710,513 + +0.6 2,710,513 + +2.6 2,710,513 + +0.3 2,710,514 - -0.0 2,710,514 - +0.7 2,710,514 - -0.3 2,710,517 + +0.3 2,710,517 + +0.2 2,710,517 + +0.0 2,710,907 + +1.0 2,710,907 + +0.3 2,710,907 + -1.3 2,710,907 + -0.7 2,710,934 + -0.3 2,710,934 + +0.3 2,710,934 + +0.6 2,710,934 + +0.6 2,710,934 + -0.5 2,710,934 + -0.4 2,710,934 + +0.5 2,710,934 + -1.0 2,711,025 + -1.6 2,711,025 + +1.0 2,711,025 + +0.0 2,711,025 + +0.8 2,711,025 + -2.1 2,711,025 + +0.6 2,711,025 + +0.0 2,711,025 + +0.2 2,711,025 + -1.0 2,711,026 - -1.0 2,711,285 + Pf1N1B4_2394 0.17 -1.3 2,711,285 + Pf1N1B4_2394 0.17 -0.3 2,711,285 + Pf1N1B4_2394 0.17 +0.3 2,711,493 + Pf1N1B4_2394 0.29 +1.1 2,711,493 + Pf1N1B4_2394 0.29 -0.3 2,711,494 - Pf1N1B4_2394 0.30 -0.8 2,711,494 - Pf1N1B4_2394 0.30 -0.7 2,711,594 + Pf1N1B4_2394 0.35 -0.6 2,711,829 + Pf1N1B4_2394 0.49 -1.5 2,711,830 - Pf1N1B4_2394 0.50 -0.4 2,711,920 + Pf1N1B4_2394 0.55 -1.7 2,711,921 - Pf1N1B4_2394 0.55 -0.3 2,711,921 - Pf1N1B4_2394 0.55 -1.6 2,711,930 + Pf1N1B4_2394 0.55 -0.5 2,711,931 - Pf1N1B4_2394 0.56 -1.1 2,712,105 + Pf1N1B4_2394 0.66 -1.3 2,712,105 + Pf1N1B4_2394 0.66 +0.6 2,712,105 + Pf1N1B4_2394 0.66 +1.9 2,712,106 - Pf1N1B4_2394 0.66 -0.8 2,712,127 + Pf1N1B4_2394 0.67 +0.3 2,712,128 - Pf1N1B4_2394 0.67 +0.3 2,712,128 - Pf1N1B4_2394 0.67 +0.5 2,712,268 + Pf1N1B4_2394 0.76 -2.1 2,712,487 + Pf1N1B4_2394 0.89 +0.7 2,712,487 + Pf1N1B4_2394 0.89 -1.4 2,712,487 + Pf1N1B4_2394 0.89 -1.5 2,712,488 - Pf1N1B4_2394 0.89 -0.8 2,712,526 + -1.8 2,712,526 + -0.5 2,712,526 + +0.3 2,712,526 + +0.0 2,712,527 - +1.0 2,712,713 + -0.8 2,712,713 + -0.8 2,712,713 + -1.5 2,712,714 - +0.1 2,712,714 - -0.3 2,712,714 - +0.6 2,712,714 - -0.7 2,712,832 - -1.1 2,712,972 + -0.4 2,712,973 - -1.1 2,712,973 - -0.8
Or see this region's nucleotide sequence