Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2336

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2334 and Pf1N1B4_2335 overlap by 4 nucleotidesPf1N1B4_2335 and Pf1N1B4_2336 overlap by 4 nucleotidesPf1N1B4_2336 and Pf1N1B4_2337 are separated by 26 nucleotidesPf1N1B4_2337 and Pf1N1B4_2338 are separated by 7 nucleotides Pf1N1B4_2334 - Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB, at 2,651,846 to 2,653,051 _2334 Pf1N1B4_2335 - Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15), at 2,653,048 to 2,653,644 _2335 Pf1N1B4_2336 - FIG003551: hypothetical protein, at 2,653,641 to 2,654,075 _2336 Pf1N1B4_2337 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,102 to 2,654,722 _2337 Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869 _2338 Position (kb) 2653 2654 2655Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2652.822 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.870 kb on + strand, within Pf1N1B4_2334at 2652.871 kb on - strand, within Pf1N1B4_2334at 2652.921 kb on + strand, within Pf1N1B4_2334at 2652.922 kb on - strand, within Pf1N1B4_2334at 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.957 kb on + strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2652.958 kb on - strandat 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.125 kb on + strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.126 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.389 kb on - strand, within Pf1N1B4_2335at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strandat 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.756 kb on + strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.757 kb on - strand, within Pf1N1B4_2336at 2653.759 kb on + strand, within Pf1N1B4_2336at 2653.856 kb on + strandat 2653.857 kb on - strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.963 kb on + strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2653.964 kb on - strand, within Pf1N1B4_2336at 2654.048 kb on + strandat 2654.049 kb on - strandat 2654.049 kb on - strandat 2654.052 kb on + strandat 2654.052 kb on + strandat 2654.053 kb on - strandat 2654.064 kb on + strandat 2654.064 kb on + strandat 2654.165 kb on + strand, within Pf1N1B4_2337at 2654.174 kb on - strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.180 kb on + strand, within Pf1N1B4_2337at 2654.181 kb on - strand, within Pf1N1B4_2337at 2654.181 kb on - strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.307 kb on + strand, within Pf1N1B4_2337at 2654.308 kb on - strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strand, within Pf1N1B4_2337at 2654.316 kb on + strandat 2654.317 kb on - strand, within Pf1N1B4_2337at 2654.317 kb on - strand, within Pf1N1B4_2337at 2654.451 kb on + strand, within Pf1N1B4_2337at 2654.739 kb on + strandat 2654.739 kb on + strandat 2654.741 kb on + strandat 2654.741 kb on + strandat 2654.742 kb on - strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.752 kb on + strandat 2654.753 kb on - strandat 2654.753 kb on - strandat 2654.773 kb on + strandat 2654.773 kb on + strandat 2654.773 kb on + strandat 2654.773 kb on + strandat 2654.993 kb on - strand, within Pf1N1B4_2338at 2654.993 kb on - strand, within Pf1N1B4_2338at 2654.993 kb on - strand, within Pf1N1B4_2338at 2655.019 kb on + strand, within Pf1N1B4_2338at 2655.019 kb on + strand, within Pf1N1B4_2338at 2655.019 kb on + strand, within Pf1N1B4_2338at 2655.019 kb on + strand, within Pf1N1B4_2338at 2655.019 kb on + strand, within Pf1N1B4_2338at 2655.020 kb on - strand, within Pf1N1B4_2338at 2655.020 kb on - strand, within Pf1N1B4_2338at 2655.062 kb on - strand, within Pf1N1B4_2338at 2655.070 kb on + strand, within Pf1N1B4_2338at 2655.070 kb on + strand, within Pf1N1B4_2338at 2655.070 kb on + strand, within Pf1N1B4_2338at 2655.070 kb on + strand, within Pf1N1B4_2338at 2655.071 kb on - strand, within Pf1N1B4_2338at 2655.071 kb on - strand, within Pf1N1B4_2338

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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2,652,822 + Pf1N1B4_2334 0.81 -0.6
2,652,870 + Pf1N1B4_2334 0.85 -0.0
2,652,870 + Pf1N1B4_2334 0.85 +1.1
2,652,871 - Pf1N1B4_2334 0.85 +2.0
2,652,921 + Pf1N1B4_2334 0.89 -1.3
2,652,922 - Pf1N1B4_2334 0.89 -1.4
2,652,957 + +1.0
2,652,957 + +1.4
2,652,957 + -0.6
2,652,958 - +1.4
2,652,958 - +1.7
2,652,958 - -2.1
2,653,125 + Pf1N1B4_2335 0.13 -1.2
2,653,125 + Pf1N1B4_2335 0.13 -0.2
2,653,126 - Pf1N1B4_2335 0.13 +0.4
2,653,126 - Pf1N1B4_2335 0.13 -0.2
2,653,126 - Pf1N1B4_2335 0.13 -1.0
2,653,126 - Pf1N1B4_2335 0.13 +0.8
2,653,389 - Pf1N1B4_2335 0.57 -1.3
2,653,389 - Pf1N1B4_2335 0.57 -1.3
2,653,389 - Pf1N1B4_2335 0.57 +0.6
2,653,756 + Pf1N1B4_2336 0.26 -0.2
2,653,756 + Pf1N1B4_2336 0.26 +2.2
2,653,756 + Pf1N1B4_2336 0.26 +1.1
2,653,756 + +1.4
2,653,756 + Pf1N1B4_2336 0.26 +1.6
2,653,756 + Pf1N1B4_2336 0.26 -0.4
2,653,756 + Pf1N1B4_2336 0.26 +1.0
2,653,757 - Pf1N1B4_2336 0.27 +0.4
2,653,757 - Pf1N1B4_2336 0.27 +0.8
2,653,759 + Pf1N1B4_2336 0.27 +0.8
2,653,856 + +1.0
2,653,857 - Pf1N1B4_2336 0.50 +1.0
2,653,963 + Pf1N1B4_2336 0.74 +3.7
2,653,963 + Pf1N1B4_2336 0.74 +0.2
2,653,963 + Pf1N1B4_2336 0.74 +1.7
2,653,963 + Pf1N1B4_2336 0.74 -0.8
2,653,963 + Pf1N1B4_2336 0.74 +0.7
2,653,963 + Pf1N1B4_2336 0.74 +0.7
2,653,964 - Pf1N1B4_2336 0.74 +0.2
2,653,964 - Pf1N1B4_2336 0.74 -0.2
2,654,048 + +3.7
2,654,049 - -1.8
2,654,049 - +1.4
2,654,052 + -0.0
2,654,052 + +0.2
2,654,053 - -0.2
2,654,064 + +2.1
2,654,064 + +0.2
2,654,165 + Pf1N1B4_2337 0.10 -1.0
2,654,174 - Pf1N1B4_2337 0.12 +0.4
2,654,180 + Pf1N1B4_2337 0.13 +0.3
2,654,180 + Pf1N1B4_2337 0.13 +0.0
2,654,180 + Pf1N1B4_2337 0.13 +1.8
2,654,180 + Pf1N1B4_2337 0.13 +0.4
2,654,181 - Pf1N1B4_2337 0.13 -0.7
2,654,181 - Pf1N1B4_2337 0.13 -2.1
2,654,307 + Pf1N1B4_2337 0.33 -0.0
2,654,307 + Pf1N1B4_2337 0.33 +0.6
2,654,307 + Pf1N1B4_2337 0.33 +0.4
2,654,308 - Pf1N1B4_2337 0.33 -1.0
2,654,316 + Pf1N1B4_2337 0.34 +0.8
2,654,316 + Pf1N1B4_2337 0.34 +0.2
2,654,316 + Pf1N1B4_2337 0.34 -0.2
2,654,316 + Pf1N1B4_2337 0.34 -1.0
2,654,316 + Pf1N1B4_2337 0.34 -0.0
2,654,316 + Pf1N1B4_2337 0.34 +0.4
2,654,316 + -0.6
2,654,317 - Pf1N1B4_2337 0.35 -0.2
2,654,317 - Pf1N1B4_2337 0.35 +0.2
2,654,451 + Pf1N1B4_2337 0.56 -1.2
2,654,739 + -0.8
2,654,739 + +0.5
2,654,741 + -0.9
2,654,741 + +0.1
2,654,742 - -2.1
2,654,752 + +2.4
2,654,752 + +0.6
2,654,752 + +0.7
2,654,752 + -0.5
2,654,752 + +0.4
2,654,752 + +0.7
2,654,752 + +1.9
2,654,752 + -1.0
2,654,752 + +0.1
2,654,753 - -1.0
2,654,753 - -0.5
2,654,773 + +0.6
2,654,773 + +0.6
2,654,773 + +0.6
2,654,773 + +1.4
2,654,993 - Pf1N1B4_2338 0.23 -1.3
2,654,993 - Pf1N1B4_2338 0.23 +0.4
2,654,993 - Pf1N1B4_2338 0.23 -0.6
2,655,019 + Pf1N1B4_2338 0.25 +0.1
2,655,019 + Pf1N1B4_2338 0.25 +0.8
2,655,019 + Pf1N1B4_2338 0.25 -0.2
2,655,019 + Pf1N1B4_2338 0.25 -0.9
2,655,019 + Pf1N1B4_2338 0.25 -0.6
2,655,020 - Pf1N1B4_2338 0.25 +0.4
2,655,020 - Pf1N1B4_2338 0.25 -0.4
2,655,062 - Pf1N1B4_2338 0.29 -0.4
2,655,070 + Pf1N1B4_2338 0.30 +0.5
2,655,070 + Pf1N1B4_2338 0.30 +1.0
2,655,070 + Pf1N1B4_2338 0.30 +0.8
2,655,070 + Pf1N1B4_2338 0.30 -1.6
2,655,071 - Pf1N1B4_2338 0.30 -0.1
2,655,071 - Pf1N1B4_2338 0.30 +3.4

Or see this region's nucleotide sequence