Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_217 and Pf1N1B4_218 are separated by 112 nucleotides Pf1N1B4_218 and Pf1N1B4_219 are separated by 144 nucleotides Pf1N1B4_219 and Pf1N1B4_220 are separated by 178 nucleotides
Pf1N1B4_217 - Hypothetical protein PvdY @ Siderophore synthetase small component, acetyltransferase, at 276,453 to 277,466
_217
Pf1N1B4_218 - Periplasmic binding protein, at 277,579 to 278,322
_218
Pf1N1B4_219 - Acyclic terpenes utilization regulator AtuR, TetR family, at 278,467 to 279,093
_219
Pf1N1B4_220 - 3-hydroxy-3isohexenylglutaryl-CoA:acetate lyase, at 279,272 to 281,074
_220
Position (kb) 277
278
279 Strain fitness (log2 ratio) -2
-1
0
1
2 at 276.640 kb on + strand, within Pf1N1B4_217 at 276.641 kb on - strand, within Pf1N1B4_217 at 276.641 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand at 276.743 kb on - strand, within Pf1N1B4_217 at 276.743 kb on - strand at 276.743 kb on - strand, within Pf1N1B4_217 at 276.775 kb on + strand, within Pf1N1B4_217 at 276.776 kb on - strand, within Pf1N1B4_217 at 276.776 kb on - strand, within Pf1N1B4_217 at 276.787 kb on + strand, within Pf1N1B4_217 at 276.788 kb on - strand, within Pf1N1B4_217 at 276.788 kb on - strand, within Pf1N1B4_217 at 276.831 kb on - strand at 276.831 kb on - strand, within Pf1N1B4_217 at 276.860 kb on - strand, within Pf1N1B4_217 at 276.961 kb on - strand, within Pf1N1B4_217 at 277.081 kb on + strand, within Pf1N1B4_217 at 277.588 kb on - strand at 277.614 kb on - strand at 277.614 kb on - strand at 277.725 kb on - strand, within Pf1N1B4_218 at 277.725 kb on - strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.799 kb on + strand, within Pf1N1B4_218 at 277.800 kb on - strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.868 kb on + strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.869 kb on - strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand at 277.886 kb on + strand, within Pf1N1B4_218 at 277.886 kb on + strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand, within Pf1N1B4_218 at 277.887 kb on - strand at 277.887 kb on - strand at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand, within Pf1N1B4_218 at 277.902 kb on - strand at 277.935 kb on - strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.985 kb on + strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 277.986 kb on - strand, within Pf1N1B4_218 at 278.255 kb on + strand at 278.256 kb on - strand at 278.256 kb on - strand at 278.385 kb on - strand at 278.385 kb on - strand at 278.440 kb on - strand at 278.660 kb on + strand, within Pf1N1B4_219 at 278.661 kb on - strand, within Pf1N1B4_219 at 278.664 kb on + strand, within Pf1N1B4_219 at 278.720 kb on + strand, within Pf1N1B4_219 at 278.721 kb on - strand, within Pf1N1B4_219 at 278.721 kb on - strand at 278.721 kb on - strand, within Pf1N1B4_219 at 278.849 kb on + strand, within Pf1N1B4_219 at 278.914 kb on - strand, within Pf1N1B4_219 at 278.969 kb on + strand, within Pf1N1B4_219 at 279.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 276,640 + Pf1N1B4_217 0.18 +0.8 276,641 - Pf1N1B4_217 0.19 -0.8 276,641 - Pf1N1B4_217 0.19 +0.1 276,743 - Pf1N1B4_217 0.29 -0.9 276,743 - Pf1N1B4_217 0.29 -1.5 276,743 - -0.2 276,743 - Pf1N1B4_217 0.29 -1.7 276,743 - +0.2 276,743 - Pf1N1B4_217 0.29 +1.4 276,775 + Pf1N1B4_217 0.32 +0.0 276,776 - Pf1N1B4_217 0.32 +0.6 276,776 - Pf1N1B4_217 0.32 -1.5 276,787 + Pf1N1B4_217 0.33 +0.0 276,788 - Pf1N1B4_217 0.33 -1.5 276,788 - Pf1N1B4_217 0.33 -0.8 276,831 - +0.8 276,831 - Pf1N1B4_217 0.37 -0.2 276,860 - Pf1N1B4_217 0.40 -0.0 276,961 - Pf1N1B4_217 0.50 -1.2 277,081 + Pf1N1B4_217 0.62 -1.2 277,588 - +2.1 277,614 - -0.2 277,614 - +0.1 277,725 - Pf1N1B4_218 0.20 -0.2 277,725 - Pf1N1B4_218 0.20 -1.1 277,799 + Pf1N1B4_218 0.30 -0.1 277,799 + Pf1N1B4_218 0.30 -0.5 277,800 - Pf1N1B4_218 0.30 -0.4 277,868 + Pf1N1B4_218 0.39 -1.2 277,868 + Pf1N1B4_218 0.39 +1.8 277,869 - Pf1N1B4_218 0.39 -0.6 277,869 - Pf1N1B4_218 0.39 -2.2 277,869 - Pf1N1B4_218 0.39 -1.4 277,886 + Pf1N1B4_218 0.41 -0.6 277,886 + +1.4 277,886 + Pf1N1B4_218 0.41 +0.1 277,886 + Pf1N1B4_218 0.41 -0.5 277,887 - +0.4 277,887 - Pf1N1B4_218 0.41 +0.8 277,887 - Pf1N1B4_218 0.41 -1.5 277,887 - -0.8 277,887 - +1.8 277,902 - Pf1N1B4_218 0.43 -0.8 277,902 - Pf1N1B4_218 0.43 -0.6 277,902 - +0.8 277,935 - Pf1N1B4_218 0.48 +0.4 277,985 + Pf1N1B4_218 0.55 -0.5 277,985 + Pf1N1B4_218 0.55 -0.2 277,986 - Pf1N1B4_218 0.55 +0.2 277,986 - Pf1N1B4_218 0.55 -1.3 277,986 - Pf1N1B4_218 0.55 -0.6 278,255 + -1.5 278,256 - -1.8 278,256 - -2.2 278,385 - -0.1 278,385 - +0.0 278,440 - +2.1 278,660 + Pf1N1B4_219 0.31 +1.4 278,661 - Pf1N1B4_219 0.31 +0.2 278,664 + Pf1N1B4_219 0.31 +0.5 278,720 + Pf1N1B4_219 0.40 +0.2 278,721 - Pf1N1B4_219 0.41 +0.2 278,721 - -0.2 278,721 - Pf1N1B4_219 0.41 -0.6 278,849 + Pf1N1B4_219 0.61 -0.2 278,914 - Pf1N1B4_219 0.71 -0.0 278,969 + Pf1N1B4_219 0.80 -0.1 279,106 - +0.8
Or see this region's nucleotide sequence