Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2081 and Pf1N1B4_2082 are separated by 726 nucleotides Pf1N1B4_2082 and Pf1N1B4_2083 are separated by 32 nucleotides Pf1N1B4_2083 and Pf1N1B4_2084 are separated by 28 nucleotides
Pf1N1B4_2081 - VgrG protein, at 2,379,721 to 2,381,748
_2081
Pf1N1B4_2082 - Uncharacterized protein ImpA, at 2,382,475 to 2,384,031
_2082
Pf1N1B4_2083 - Uncharacterized protein ImpB, at 2,384,064 to 2,384,567
_2083
Pf1N1B4_2084 - Uncharacterized protein ImpC, at 2,384,596 to 2,386,071
_2084
Position (kb)
2382
2383
2384
2385 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2381.642 kb on + strand at 2381.642 kb on + strand at 2381.642 kb on + strand at 2381.642 kb on + strand at 2381.643 kb on - strand at 2381.900 kb on - strand at 2381.900 kb on - strand at 2381.993 kb on + strand at 2381.993 kb on + strand at 2381.993 kb on + strand at 2381.994 kb on - strand at 2382.070 kb on + strand at 2382.070 kb on + strand at 2382.071 kb on - strand at 2382.071 kb on - strand at 2382.071 kb on - strand at 2382.095 kb on + strand at 2382.117 kb on - strand at 2382.124 kb on - strand at 2382.272 kb on + strand at 2382.272 kb on + strand at 2382.273 kb on - strand at 2382.273 kb on - strand at 2382.273 kb on - strand at 2382.273 kb on - strand at 2382.273 kb on - strand at 2382.317 kb on + strand at 2382.352 kb on - strand at 2382.453 kb on + strand at 2382.463 kb on + strand at 2382.506 kb on - strand at 2382.577 kb on + strand at 2382.578 kb on - strand at 2382.578 kb on - strand at 2382.578 kb on - strand at 2382.727 kb on + strand, within Pf1N1B4_2082 at 2383.363 kb on + strand, within Pf1N1B4_2082 at 2383.364 kb on - strand, within Pf1N1B4_2082 at 2383.364 kb on - strand, within Pf1N1B4_2082 at 2383.379 kb on + strand, within Pf1N1B4_2082 at 2383.379 kb on + strand at 2383.379 kb on + strand, within Pf1N1B4_2082 at 2383.433 kb on + strand, within Pf1N1B4_2082 at 2383.434 kb on - strand, within Pf1N1B4_2082 at 2383.434 kb on - strand, within Pf1N1B4_2082 at 2383.822 kb on + strand, within Pf1N1B4_2082 at 2383.823 kb on - strand, within Pf1N1B4_2082 at 2383.866 kb on - strand, within Pf1N1B4_2082 at 2383.866 kb on - strand, within Pf1N1B4_2082 at 2383.929 kb on + strand at 2383.929 kb on + strand at 2383.929 kb on + strand at 2383.930 kb on - strand at 2383.930 kb on - strand at 2383.987 kb on + strand at 2384.021 kb on + strand at 2384.030 kb on - strand at 2384.030 kb on - strand at 2384.102 kb on + strand at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.164 kb on + strand, within Pf1N1B4_2083 at 2384.165 kb on - strand, within Pf1N1B4_2083 at 2384.181 kb on + strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.182 kb on - strand, within Pf1N1B4_2083 at 2384.574 kb on - strand at 2384.625 kb on + strand at 2384.641 kb on + strand at 2384.641 kb on + strand at 2384.641 kb on + strand at 2384.699 kb on - strand at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.010 kb on + strand, within Pf1N1B4_2084 at 2385.011 kb on - strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.022 kb on + strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084 at 2385.023 kb on - strand, within Pf1N1B4_2084
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 2,381,642 + -0.1 2,381,642 + -1.4 2,381,642 + -1.7 2,381,642 + -0.1 2,381,643 - -0.7 2,381,900 - -1.1 2,381,900 - -0.9 2,381,993 + +0.5 2,381,993 + -1.3 2,381,993 + +0.4 2,381,994 - +0.1 2,382,070 + +0.5 2,382,070 + -0.7 2,382,071 - +0.9 2,382,071 - -0.9 2,382,071 - +0.9 2,382,095 + -0.3 2,382,117 - +0.7 2,382,124 - +0.1 2,382,272 + -0.5 2,382,272 + -1.3 2,382,273 - -1.7 2,382,273 - +0.1 2,382,273 - +1.5 2,382,273 - -0.1 2,382,273 - +0.5 2,382,317 + +0.7 2,382,352 - -0.1 2,382,453 + -0.1 2,382,463 + -0.9 2,382,506 - +0.5 2,382,577 + +0.5 2,382,578 - -0.5 2,382,578 - +1.5 2,382,578 - -0.5 2,382,727 + Pf1N1B4_2082 0.16 +2.5 2,383,363 + Pf1N1B4_2082 0.57 -2.1 2,383,364 - Pf1N1B4_2082 0.57 -0.1 2,383,364 - Pf1N1B4_2082 0.57 -1.1 2,383,379 + Pf1N1B4_2082 0.58 -0.8 2,383,379 + +1.5 2,383,379 + Pf1N1B4_2082 0.58 -1.5 2,383,433 + Pf1N1B4_2082 0.62 -0.7 2,383,434 - Pf1N1B4_2082 0.62 +0.5 2,383,434 - Pf1N1B4_2082 0.62 +0.5 2,383,822 + Pf1N1B4_2082 0.87 -1.3 2,383,823 - Pf1N1B4_2082 0.87 -0.9 2,383,866 - Pf1N1B4_2082 0.89 +0.9 2,383,866 - Pf1N1B4_2082 0.89 -1.5 2,383,929 + -0.7 2,383,929 + -1.7 2,383,929 + +0.1 2,383,930 - +0.9 2,383,930 - -1.1 2,383,987 + +1.2 2,384,021 + -1.0 2,384,030 - +0.9 2,384,030 - +0.7 2,384,102 + -0.1 2,384,164 + Pf1N1B4_2083 0.20 +0.4 2,384,164 + Pf1N1B4_2083 0.20 +0.9 2,384,164 + Pf1N1B4_2083 0.20 -0.9 2,384,164 + Pf1N1B4_2083 0.20 -1.1 2,384,165 - Pf1N1B4_2083 0.20 -1.1 2,384,181 + Pf1N1B4_2083 0.23 -0.0 2,384,182 - Pf1N1B4_2083 0.23 -0.8 2,384,182 - Pf1N1B4_2083 0.23 -1.5 2,384,182 - Pf1N1B4_2083 0.23 -1.0 2,384,574 - -1.0 2,384,625 + +0.2 2,384,641 + +0.3 2,384,641 + +0.3 2,384,641 + +1.5 2,384,699 - -0.3 2,385,010 + Pf1N1B4_2084 0.28 -0.4 2,385,010 + Pf1N1B4_2084 0.28 -0.5 2,385,010 + Pf1N1B4_2084 0.28 -0.5 2,385,011 - Pf1N1B4_2084 0.28 -0.9 2,385,022 + Pf1N1B4_2084 0.29 +0.5 2,385,022 + Pf1N1B4_2084 0.29 -0.1 2,385,022 + Pf1N1B4_2084 0.29 -0.1 2,385,023 - Pf1N1B4_2084 0.29 +1.0 2,385,023 - Pf1N1B4_2084 0.29 -0.5 2,385,023 - Pf1N1B4_2084 0.29 +0.8 2,385,023 - Pf1N1B4_2084 0.29 +1.2 2,385,023 - Pf1N1B4_2084 0.29 +1.1
Or see this region's nucleotide sequence