Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1925 and Pf1N1B4_1926 are separated by 72 nucleotides Pf1N1B4_1926 and Pf1N1B4_1927 are separated by 44 nucleotides
Pf1N1B4_1925 - Two-component response regulator, at 2,201,388 to 2,202,107
_1925
Pf1N1B4_1926 - DNA gyrase subunit B (EC 5.99.1.3), at 2,202,180 to 2,204,558
_1926
Pf1N1B4_1927 - DNA recombination and repair protein RecF, at 2,204,603 to 2,205,706
_1927
Position (kb)
2202
2203
2204
2205 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.280 kb on + strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.281 kb on - strand at 2201.333 kb on + strand at 2201.364 kb on - strand at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.489 kb on + strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.490 kb on - strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.700 kb on + strand, within Pf1N1B4_1925 at 2201.701 kb on - strand, within Pf1N1B4_1925 at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.794 kb on + strand at 2201.794 kb on + strand, within Pf1N1B4_1925 at 2201.795 kb on - strand, within Pf1N1B4_1925 at 2201.795 kb on - strand, within Pf1N1B4_1925 at 2201.881 kb on - strand, within Pf1N1B4_1925 at 2201.934 kb on + strand, within Pf1N1B4_1925 at 2201.934 kb on + strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.935 kb on - strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.940 kb on + strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.941 kb on - strand, within Pf1N1B4_1925 at 2201.968 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.996 kb on + strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2201.997 kb on - strand, within Pf1N1B4_1925 at 2202.001 kb on - strand, within Pf1N1B4_1925 at 2202.060 kb on + strand at 2202.060 kb on + strand at 2202.060 kb on + strand at 2202.061 kb on - strand at 2202.061 kb on - strand at 2202.127 kb on - strand at 2202.142 kb on + strand at 2204.694 kb on - strand at 2204.694 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.706 kb on - strand at 2204.714 kb on - strand, within Pf1N1B4_1927 at 2204.714 kb on - strand, within Pf1N1B4_1927 at 2204.714 kb on - strand, within Pf1N1B4_1927 at 2204.714 kb on - strand, within Pf1N1B4_1927 at 2204.717 kb on - strand, within Pf1N1B4_1927 at 2204.717 kb on - strand, within Pf1N1B4_1927 at 2204.717 kb on - strand, within Pf1N1B4_1927 at 2204.717 kb on - strand, within Pf1N1B4_1927 at 2204.733 kb on - strand, within Pf1N1B4_1927 at 2204.733 kb on - strand, within Pf1N1B4_1927 at 2204.733 kb on - strand, within Pf1N1B4_1927 at 2204.806 kb on - strand, within Pf1N1B4_1927 at 2204.806 kb on - strand, within Pf1N1B4_1927 at 2204.806 kb on - strand, within Pf1N1B4_1927 at 2204.806 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.810 kb on - strand, within Pf1N1B4_1927 at 2204.912 kb on - strand, within Pf1N1B4_1927 at 2204.912 kb on - strand, within Pf1N1B4_1927 at 2204.912 kb on - strand, within Pf1N1B4_1927 at 2204.927 kb on - strand, within Pf1N1B4_1927 at 2205.043 kb on - strand, within Pf1N1B4_1927 at 2205.043 kb on - strand, within Pf1N1B4_1927 at 2205.043 kb on - strand, within Pf1N1B4_1927 at 2205.043 kb on - strand, within Pf1N1B4_1927 at 2205.118 kb on - strand, within Pf1N1B4_1927 at 2205.118 kb on - strand, within Pf1N1B4_1927 at 2205.118 kb on - strand, within Pf1N1B4_1927 at 2205.118 kb on - strand, within Pf1N1B4_1927 at 2205.118 kb on - strand, within Pf1N1B4_1927 at 2205.138 kb on - strand, within Pf1N1B4_1927 at 2205.138 kb on - strand, within Pf1N1B4_1927 at 2205.200 kb on - strand, within Pf1N1B4_1927 at 2205.449 kb on + strand, within Pf1N1B4_1927 at 2205.449 kb on + strand, within Pf1N1B4_1927 at 2205.449 kb on + strand, within Pf1N1B4_1927 at 2205.502 kb on + strand, within Pf1N1B4_1927 at 2205.502 kb on + strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.503 kb on - strand, within Pf1N1B4_1927 at 2205.534 kb on - strand, within Pf1N1B4_1927 at 2205.536 kb on + strand, within Pf1N1B4_1927 at 2205.536 kb on + strand, within Pf1N1B4_1927 at 2205.536 kb on + strand, within Pf1N1B4_1927 at 2205.537 kb on - strand, within Pf1N1B4_1927 at 2205.547 kb on + strand, within Pf1N1B4_1927
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 2,201,280 + +0.3 2,201,280 + -0.3 2,201,280 + +1.5 2,201,280 + -1.8 2,201,280 + -0.1 2,201,281 - +0.3 2,201,281 - -0.1 2,201,281 - +0.1 2,201,281 - -0.3 2,201,281 - -1.7 2,201,281 - -0.5 2,201,333 + +0.7 2,201,364 - -0.3 2,201,489 + Pf1N1B4_1925 0.14 +1.5 2,201,489 + Pf1N1B4_1925 0.14 +0.2 2,201,489 + Pf1N1B4_1925 0.14 +0.1 2,201,489 + Pf1N1B4_1925 0.14 +1.0 2,201,489 + Pf1N1B4_1925 0.14 -1.1 2,201,489 + Pf1N1B4_1925 0.14 -0.3 2,201,489 + Pf1N1B4_1925 0.14 +2.1 2,201,489 + Pf1N1B4_1925 0.14 -0.8 2,201,489 + Pf1N1B4_1925 0.14 -1.1 2,201,489 + Pf1N1B4_1925 0.14 -0.5 2,201,490 - Pf1N1B4_1925 0.14 +0.5 2,201,490 - Pf1N1B4_1925 0.14 -1.7 2,201,490 - Pf1N1B4_1925 0.14 -1.7 2,201,700 + Pf1N1B4_1925 0.43 -1.1 2,201,700 + Pf1N1B4_1925 0.43 -0.1 2,201,700 + Pf1N1B4_1925 0.43 +0.5 2,201,701 - Pf1N1B4_1925 0.43 -1.5 2,201,794 + Pf1N1B4_1925 0.56 -1.2 2,201,794 + Pf1N1B4_1925 0.56 +0.9 2,201,794 + +0.5 2,201,794 + Pf1N1B4_1925 0.56 -1.2 2,201,795 - Pf1N1B4_1925 0.57 -0.6 2,201,795 - Pf1N1B4_1925 0.57 +0.9 2,201,881 - Pf1N1B4_1925 0.68 +0.9 2,201,934 + Pf1N1B4_1925 0.76 +0.5 2,201,934 + Pf1N1B4_1925 0.76 +0.0 2,201,935 - Pf1N1B4_1925 0.76 +0.2 2,201,935 - Pf1N1B4_1925 0.76 -0.1 2,201,935 - Pf1N1B4_1925 0.76 +1.5 2,201,940 + Pf1N1B4_1925 0.77 +0.9 2,201,940 + Pf1N1B4_1925 0.77 +0.5 2,201,940 + Pf1N1B4_1925 0.77 +0.1 2,201,940 + Pf1N1B4_1925 0.77 -0.1 2,201,940 + Pf1N1B4_1925 0.77 -0.1 2,201,940 + Pf1N1B4_1925 0.77 +0.2 2,201,940 + Pf1N1B4_1925 0.77 +0.6 2,201,940 + Pf1N1B4_1925 0.77 -1.3 2,201,940 + Pf1N1B4_1925 0.77 -1.5 2,201,940 + Pf1N1B4_1925 0.77 +0.2 2,201,941 - Pf1N1B4_1925 0.77 +0.7 2,201,941 - Pf1N1B4_1925 0.77 -0.1 2,201,941 - Pf1N1B4_1925 0.77 -0.4 2,201,941 - Pf1N1B4_1925 0.77 -0.1 2,201,941 - Pf1N1B4_1925 0.77 -0.4 2,201,941 - Pf1N1B4_1925 0.77 +0.2 2,201,941 - Pf1N1B4_1925 0.77 +0.6 2,201,941 - Pf1N1B4_1925 0.77 +1.9 2,201,941 - Pf1N1B4_1925 0.77 +1.5 2,201,968 + Pf1N1B4_1925 0.81 -0.5 2,201,996 + Pf1N1B4_1925 0.84 -0.3 2,201,996 + Pf1N1B4_1925 0.84 +0.9 2,201,996 + Pf1N1B4_1925 0.84 -0.2 2,201,996 + Pf1N1B4_1925 0.84 +0.6 2,201,996 + Pf1N1B4_1925 0.84 -1.8 2,201,996 + Pf1N1B4_1925 0.84 -0.5 2,201,996 + Pf1N1B4_1925 0.84 -2.5 2,201,996 + Pf1N1B4_1925 0.84 -1.5 2,201,996 + Pf1N1B4_1925 0.84 +1.5 2,201,996 + Pf1N1B4_1925 0.84 +0.5 2,201,996 + Pf1N1B4_1925 0.84 -0.1 2,201,996 + Pf1N1B4_1925 0.84 +0.5 2,201,996 + Pf1N1B4_1925 0.84 +0.3 2,201,996 + Pf1N1B4_1925 0.84 -1.4 2,201,996 + Pf1N1B4_1925 0.84 +0.5 2,201,996 + Pf1N1B4_1925 0.84 +1.5 2,201,997 - Pf1N1B4_1925 0.85 -0.8 2,201,997 - Pf1N1B4_1925 0.85 +0.9 2,201,997 - Pf1N1B4_1925 0.85 -0.3 2,201,997 - Pf1N1B4_1925 0.85 +0.1 2,201,997 - Pf1N1B4_1925 0.85 -0.1 2,201,997 - Pf1N1B4_1925 0.85 +0.7 2,201,997 - Pf1N1B4_1925 0.85 -0.7 2,201,997 - Pf1N1B4_1925 0.85 +0.9 2,201,997 - Pf1N1B4_1925 0.85 -0.1 2,201,997 - Pf1N1B4_1925 0.85 +1.3 2,201,997 - Pf1N1B4_1925 0.85 +1.8 2,201,997 - Pf1N1B4_1925 0.85 +0.4 2,201,997 - Pf1N1B4_1925 0.85 -0.4 2,202,001 - Pf1N1B4_1925 0.85 +0.1 2,202,060 + +1.2 2,202,060 + -1.3 2,202,060 + +0.2 2,202,061 - +1.1 2,202,061 - +0.5 2,202,127 - +2.2 2,202,142 + +0.7 2,204,694 - -1.5 2,204,694 - -0.8 2,204,706 - -1.9 2,204,706 - -0.5 2,204,706 - -1.5 2,204,706 - -1.1 2,204,706 - -2.2 2,204,706 - -2.3 2,204,706 - -0.1 2,204,714 - Pf1N1B4_1927 0.10 -1.7 2,204,714 - Pf1N1B4_1927 0.10 -0.8 2,204,714 - Pf1N1B4_1927 0.10 -1.4 2,204,714 - Pf1N1B4_1927 0.10 -1.3 2,204,717 - Pf1N1B4_1927 0.10 -0.4 2,204,717 - Pf1N1B4_1927 0.10 -0.8 2,204,717 - Pf1N1B4_1927 0.10 +0.5 2,204,717 - Pf1N1B4_1927 0.10 -1.7 2,204,733 - Pf1N1B4_1927 0.12 -0.1 2,204,733 - Pf1N1B4_1927 0.12 -0.1 2,204,733 - Pf1N1B4_1927 0.12 -2.9 2,204,806 - Pf1N1B4_1927 0.18 -0.9 2,204,806 - Pf1N1B4_1927 0.18 -1.7 2,204,806 - Pf1N1B4_1927 0.18 -0.5 2,204,806 - Pf1N1B4_1927 0.18 -1.1 2,204,810 - Pf1N1B4_1927 0.19 -1.0 2,204,810 - Pf1N1B4_1927 0.19 -1.7 2,204,810 - Pf1N1B4_1927 0.19 -2.1 2,204,810 - Pf1N1B4_1927 0.19 -0.7 2,204,810 - Pf1N1B4_1927 0.19 -2.1 2,204,810 - Pf1N1B4_1927 0.19 +0.2 2,204,810 - Pf1N1B4_1927 0.19 +0.2 2,204,810 - Pf1N1B4_1927 0.19 -1.3 2,204,912 - Pf1N1B4_1927 0.28 -1.3 2,204,912 - Pf1N1B4_1927 0.28 -1.5 2,204,912 - Pf1N1B4_1927 0.28 -3.0 2,204,927 - Pf1N1B4_1927 0.29 -1.3 2,205,043 - Pf1N1B4_1927 0.40 -2.0 2,205,043 - Pf1N1B4_1927 0.40 +1.5 2,205,043 - Pf1N1B4_1927 0.40 +0.5 2,205,043 - Pf1N1B4_1927 0.40 -2.8 2,205,118 - Pf1N1B4_1927 0.47 -0.5 2,205,118 - Pf1N1B4_1927 0.47 +0.4 2,205,118 - Pf1N1B4_1927 0.47 -1.0 2,205,118 - Pf1N1B4_1927 0.47 -0.8 2,205,118 - Pf1N1B4_1927 0.47 +1.9 2,205,138 - Pf1N1B4_1927 0.48 -0.2 2,205,138 - Pf1N1B4_1927 0.48 -0.8 2,205,200 - Pf1N1B4_1927 0.54 +2.1 2,205,449 + Pf1N1B4_1927 0.77 -1.1 2,205,449 + Pf1N1B4_1927 0.77 +1.7 2,205,449 + Pf1N1B4_1927 0.77 -0.5 2,205,502 + Pf1N1B4_1927 0.81 +0.6 2,205,502 + Pf1N1B4_1927 0.81 -0.1 2,205,503 - Pf1N1B4_1927 0.82 -0.5 2,205,503 - Pf1N1B4_1927 0.82 +0.5 2,205,503 - Pf1N1B4_1927 0.82 -0.8 2,205,503 - Pf1N1B4_1927 0.82 -1.1 2,205,503 - Pf1N1B4_1927 0.82 -1.3 2,205,503 - Pf1N1B4_1927 0.82 +0.3 2,205,534 - Pf1N1B4_1927 0.84 +0.7 2,205,536 + Pf1N1B4_1927 0.85 -0.8 2,205,536 + Pf1N1B4_1927 0.85 -1.1 2,205,536 + Pf1N1B4_1927 0.85 -0.9 2,205,537 - Pf1N1B4_1927 0.85 +1.5 2,205,547 + Pf1N1B4_1927 0.86 -0.7
Or see this region's nucleotide sequence