Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1584

Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1582 and Pf1N1B4_1583 are separated by 158 nucleotidesPf1N1B4_1583 and Pf1N1B4_1584 overlap by 4 nucleotidesPf1N1B4_1584 and Pf1N1B4_1585 are separated by 71 nucleotides Pf1N1B4_1582 - Histidine ammonia-lyase (EC 4.3.1.3) (from data), at 1,822,609 to 1,824,132 _1582 Pf1N1B4_1583 - Histidine ABC transporter, ATP-binding protein (TC 3.A.1), at 1,824,291 to 1,825,121 _1583 Pf1N1B4_1584 - Histidine ABC transporter, permease protein (TC 3.A.1), at 1,825,118 to 1,825,969 _1584 Pf1N1B4_1585 - Histidine ABC transporter, histidine-binding protein (TC 3.A.1), at 1,826,041 to 1,827,009 _1585 Position (kb) 1825 1826Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1824.315 kb on + strandat 1824.315 kb on + strandat 1824.315 kb on + strandat 1824.316 kb on - strandat 1824.331 kb on + strandat 1824.331 kb on + strandat 1824.331 kb on + strandat 1824.331 kb on + strandat 1824.331 kb on + strandat 1824.332 kb on - strandat 1824.332 kb on - strandat 1824.332 kb on - strandat 1824.332 kb on - strandat 1824.332 kb on - strandat 1824.332 kb on - strandat 1824.629 kb on - strand, within Pf1N1B4_1583at 1824.629 kb on - strand, within Pf1N1B4_1583at 1824.629 kb on - strand, within Pf1N1B4_1583at 1824.629 kb on - strand, within Pf1N1B4_1583at 1824.629 kb on - strand, within Pf1N1B4_1583at 1824.725 kb on - strand, within Pf1N1B4_1583at 1824.725 kb on - strand, within Pf1N1B4_1583at 1824.826 kb on + strand, within Pf1N1B4_1583at 1824.826 kb on + strand, within Pf1N1B4_1583at 1824.827 kb on - strand, within Pf1N1B4_1583at 1824.827 kb on - strand, within Pf1N1B4_1583at 1825.149 kb on + strandat 1825.149 kb on + strandat 1825.150 kb on - strandat 1825.159 kb on + strandat 1825.479 kb on + strand, within Pf1N1B4_1584at 1825.480 kb on - strand, within Pf1N1B4_1584at 1825.545 kb on + strand, within Pf1N1B4_1584at 1825.896 kb on + strandat 1825.952 kb on - strandat 1825.969 kb on + strandat 1826.042 kb on + strandat 1826.042 kb on + strandat 1826.042 kb on + strandat 1826.043 kb on - strandat 1826.043 kb on - strandat 1826.043 kb on - strandat 1826.043 kb on - strandat 1826.348 kb on + strand, within Pf1N1B4_1585at 1826.349 kb on - strand, within Pf1N1B4_1585at 1826.351 kb on + strand, within Pf1N1B4_1585at 1826.351 kb on + strand, within Pf1N1B4_1585at 1826.351 kb on + strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.352 kb on - strand, within Pf1N1B4_1585at 1826.378 kb on + strand, within Pf1N1B4_1585at 1826.378 kb on + strand, within Pf1N1B4_1585at 1826.378 kb on + strand, within Pf1N1B4_1585at 1826.379 kb on - strand, within Pf1N1B4_1585at 1826.379 kb on - strand, within Pf1N1B4_1585at 1826.379 kb on - strand, within Pf1N1B4_1585at 1826.379 kb on - strand, within Pf1N1B4_1585at 1826.435 kb on + strand, within Pf1N1B4_1585at 1826.435 kb on + strand, within Pf1N1B4_1585at 1826.435 kb on + strand, within Pf1N1B4_1585at 1826.436 kb on - strand, within Pf1N1B4_1585at 1826.436 kb on - strand, within Pf1N1B4_1585at 1826.436 kb on - strand, within Pf1N1B4_1585at 1826.453 kb on + strand, within Pf1N1B4_1585at 1826.454 kb on - strand, within Pf1N1B4_1585at 1826.454 kb on - strand, within Pf1N1B4_1585at 1826.454 kb on - strand, within Pf1N1B4_1585at 1826.510 kb on + strand, within Pf1N1B4_1585at 1826.511 kb on - strand, within Pf1N1B4_1585at 1826.511 kb on - strand, within Pf1N1B4_1585at 1826.558 kb on + strand, within Pf1N1B4_1585at 1826.558 kb on + strand, within Pf1N1B4_1585at 1826.558 kb on + strand, within Pf1N1B4_1585at 1826.558 kb on + strand, within Pf1N1B4_1585at 1826.559 kb on - strand, within Pf1N1B4_1585at 1826.559 kb on - strand, within Pf1N1B4_1585at 1826.559 kb on - strand, within Pf1N1B4_1585at 1826.559 kb on - strand, within Pf1N1B4_1585at 1826.559 kb on - strand, within Pf1N1B4_1585at 1826.649 kb on - strand, within Pf1N1B4_1585at 1826.649 kb on - strand, within Pf1N1B4_1585at 1826.649 kb on - strand, within Pf1N1B4_1585at 1826.649 kb on - strand, within Pf1N1B4_1585at 1826.649 kb on - strand, within Pf1N1B4_1585at 1826.831 kb on + strand, within Pf1N1B4_1585

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Per-strain Table

Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM
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1,824,315 + +1.4
1,824,315 + +1.6
1,824,315 + -1.0
1,824,316 - +0.9
1,824,331 + -0.6
1,824,331 + -1.0
1,824,331 + -1.8
1,824,331 + +0.8
1,824,331 + -0.6
1,824,332 - +1.4
1,824,332 - +3.1
1,824,332 - +0.2
1,824,332 - -1.0
1,824,332 - +0.2
1,824,332 - +0.5
1,824,629 - Pf1N1B4_1583 0.41 +1.4
1,824,629 - Pf1N1B4_1583 0.41 +0.8
1,824,629 - Pf1N1B4_1583 0.41 +0.7
1,824,629 - Pf1N1B4_1583 0.41 +0.8
1,824,629 - Pf1N1B4_1583 0.41 -1.0
1,824,725 - Pf1N1B4_1583 0.52 -0.8
1,824,725 - Pf1N1B4_1583 0.52 +1.4
1,824,826 + Pf1N1B4_1583 0.64 +0.6
1,824,826 + Pf1N1B4_1583 0.64 +0.2
1,824,827 - Pf1N1B4_1583 0.65 +0.6
1,824,827 - Pf1N1B4_1583 0.65 -0.2
1,825,149 + -0.5
1,825,149 + +0.5
1,825,150 - +0.2
1,825,159 + +1.4
1,825,479 + Pf1N1B4_1584 0.42 -0.0
1,825,480 - Pf1N1B4_1584 0.42 -0.8
1,825,545 + Pf1N1B4_1584 0.50 -1.2
1,825,896 + -2.1
1,825,952 - -0.6
1,825,969 + -1.2
1,826,042 + -1.8
1,826,042 + +1.9
1,826,042 + -1.4
1,826,043 - -0.4
1,826,043 - -0.5
1,826,043 - +1.1
1,826,043 - -2.3
1,826,348 + Pf1N1B4_1585 0.32 -0.6
1,826,349 - Pf1N1B4_1585 0.32 +0.0
1,826,351 + Pf1N1B4_1585 0.32 -1.0
1,826,351 + Pf1N1B4_1585 0.32 -1.1
1,826,351 + Pf1N1B4_1585 0.32 +0.5
1,826,352 - Pf1N1B4_1585 0.32 +0.1
1,826,352 - Pf1N1B4_1585 0.32 -0.1
1,826,352 - Pf1N1B4_1585 0.32 +0.1
1,826,352 - Pf1N1B4_1585 0.32 -0.2
1,826,352 - Pf1N1B4_1585 0.32 +0.1
1,826,352 - Pf1N1B4_1585 0.32 +1.4
1,826,378 + Pf1N1B4_1585 0.35 +0.8
1,826,378 + Pf1N1B4_1585 0.35 +0.2
1,826,378 + Pf1N1B4_1585 0.35 +0.8
1,826,379 - Pf1N1B4_1585 0.35 -1.6
1,826,379 - Pf1N1B4_1585 0.35 +2.2
1,826,379 - Pf1N1B4_1585 0.35 +1.4
1,826,379 - Pf1N1B4_1585 0.35 +0.7
1,826,435 + Pf1N1B4_1585 0.41 +0.2
1,826,435 + Pf1N1B4_1585 0.41 -0.9
1,826,435 + Pf1N1B4_1585 0.41 +1.1
1,826,436 - Pf1N1B4_1585 0.41 +1.4
1,826,436 - Pf1N1B4_1585 0.41 +0.4
1,826,436 - Pf1N1B4_1585 0.41 +0.0
1,826,453 + Pf1N1B4_1585 0.43 -1.5
1,826,454 - Pf1N1B4_1585 0.43 +0.7
1,826,454 - Pf1N1B4_1585 0.43 +0.4
1,826,454 - Pf1N1B4_1585 0.43 -1.5
1,826,510 + Pf1N1B4_1585 0.48 -0.6
1,826,511 - Pf1N1B4_1585 0.49 +1.9
1,826,511 - Pf1N1B4_1585 0.49 +0.4
1,826,558 + Pf1N1B4_1585 0.53 -0.8
1,826,558 + Pf1N1B4_1585 0.53 -1.8
1,826,558 + Pf1N1B4_1585 0.53 +1.1
1,826,558 + Pf1N1B4_1585 0.53 -2.1
1,826,559 - Pf1N1B4_1585 0.53 -2.3
1,826,559 - Pf1N1B4_1585 0.53 +0.2
1,826,559 - Pf1N1B4_1585 0.53 +1.2
1,826,559 - Pf1N1B4_1585 0.53 +0.5
1,826,559 - Pf1N1B4_1585 0.53 +0.9
1,826,649 - Pf1N1B4_1585 0.63 +0.6
1,826,649 - Pf1N1B4_1585 0.63 +0.7
1,826,649 - Pf1N1B4_1585 0.63 -1.5
1,826,649 - Pf1N1B4_1585 0.63 +0.4
1,826,649 - Pf1N1B4_1585 0.63 +1.0
1,826,831 + Pf1N1B4_1585 0.82 -2.6

Or see this region's nucleotide sequence