Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1277 and Pf1N1B4_1278 overlap by 4 nucleotides Pf1N1B4_1278 and Pf1N1B4_1279 are separated by 10 nucleotides Pf1N1B4_1279 and Pf1N1B4_1280 overlap by 4 nucleotides
Pf1N1B4_1277 - Flp pilus assembly protein CpaE, at 1,457,432 to 1,458,637
_1277
Pf1N1B4_1278 - Type II/IV secretion system ATP hydrolase TadA/VirB11/CpaF, TadA subfamily, at 1,458,634 to 1,459,914
_1278
Pf1N1B4_1279 - Flp pilus assembly protein TadB, at 1,459,925 to 1,460,809
_1279
Pf1N1B4_1280 - Type II/IV secretion system protein TadC, associated with Flp pilus assembly, at 1,460,806 to 1,461,696
_1280
Position (kb)
1458
1459
1460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1457.763 kb on - strand, within Pf1N1B4_1277 at 1457.763 kb on - strand, within Pf1N1B4_1277 at 1457.873 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.164 kb on + strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.165 kb on - strand, within Pf1N1B4_1277 at 1458.247 kb on + strand, within Pf1N1B4_1277 at 1458.248 kb on - strand, within Pf1N1B4_1277 at 1458.281 kb on + strand, within Pf1N1B4_1277 at 1458.281 kb on + strand, within Pf1N1B4_1277 at 1458.282 kb on - strand, within Pf1N1B4_1277 at 1458.415 kb on + strand, within Pf1N1B4_1277 at 1458.474 kb on + strand, within Pf1N1B4_1277 at 1458.475 kb on - strand, within Pf1N1B4_1277 at 1458.721 kb on + strand at 1458.814 kb on + strand, within Pf1N1B4_1278 at 1458.814 kb on + strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1458.815 kb on - strand, within Pf1N1B4_1278 at 1459.252 kb on - strand, within Pf1N1B4_1278 at 1459.415 kb on + strand, within Pf1N1B4_1278 at 1459.416 kb on - strand, within Pf1N1B4_1278 at 1459.777 kb on - strand, within Pf1N1B4_1278 at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.792 kb on + strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.793 kb on - strand at 1459.803 kb on + strand at 1459.809 kb on - strand at 1459.912 kb on + strand at 1459.912 kb on + strand at 1459.991 kb on + strand at 1459.992 kb on - strand at 1460.103 kb on + strand, within Pf1N1B4_1279 at 1460.104 kb on - strand, within Pf1N1B4_1279 at 1460.246 kb on + strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.247 kb on - strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.699 kb on + strand, within Pf1N1B4_1279 at 1460.805 kb on + strand at 1460.806 kb on - strand at 1460.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 1,457,763 - Pf1N1B4_1277 0.27 -2.5 1,457,763 - Pf1N1B4_1277 0.27 +0.0 1,457,873 + Pf1N1B4_1277 0.37 +0.5 1,458,164 + Pf1N1B4_1277 0.61 +0.5 1,458,164 + Pf1N1B4_1277 0.61 -1.1 1,458,164 + Pf1N1B4_1277 0.61 -0.5 1,458,164 + Pf1N1B4_1277 0.61 +1.2 1,458,164 + Pf1N1B4_1277 0.61 +0.1 1,458,164 + Pf1N1B4_1277 0.61 +0.1 1,458,164 + Pf1N1B4_1277 0.61 -0.3 1,458,165 - Pf1N1B4_1277 0.61 -0.1 1,458,165 - Pf1N1B4_1277 0.61 -0.5 1,458,165 - Pf1N1B4_1277 0.61 +0.5 1,458,165 - Pf1N1B4_1277 0.61 -0.1 1,458,165 - Pf1N1B4_1277 0.61 +0.6 1,458,165 - Pf1N1B4_1277 0.61 +0.7 1,458,165 - Pf1N1B4_1277 0.61 -0.7 1,458,247 + Pf1N1B4_1277 0.68 -1.6 1,458,248 - Pf1N1B4_1277 0.68 +0.4 1,458,281 + Pf1N1B4_1277 0.70 -0.1 1,458,281 + Pf1N1B4_1277 0.70 +1.1 1,458,282 - Pf1N1B4_1277 0.70 +0.6 1,458,415 + Pf1N1B4_1277 0.82 -0.6 1,458,474 + Pf1N1B4_1277 0.86 +0.9 1,458,475 - Pf1N1B4_1277 0.86 -0.5 1,458,721 + -0.3 1,458,814 + Pf1N1B4_1278 0.14 -0.7 1,458,814 + Pf1N1B4_1278 0.14 +1.7 1,458,815 - Pf1N1B4_1278 0.14 -0.4 1,458,815 - Pf1N1B4_1278 0.14 -2.3 1,458,815 - Pf1N1B4_1278 0.14 -1.9 1,458,815 - Pf1N1B4_1278 0.14 +0.5 1,458,815 - Pf1N1B4_1278 0.14 -1.7 1,459,252 - Pf1N1B4_1278 0.48 -1.9 1,459,415 + Pf1N1B4_1278 0.61 +0.1 1,459,416 - Pf1N1B4_1278 0.61 -0.3 1,459,777 - Pf1N1B4_1278 0.89 -1.2 1,459,792 + -0.7 1,459,792 + -0.3 1,459,792 + +0.3 1,459,792 + +0.2 1,459,792 + +0.1 1,459,792 + -0.1 1,459,793 - -0.4 1,459,793 - -1.1 1,459,793 - +3.8 1,459,793 - +1.3 1,459,793 - +1.0 1,459,793 - -0.3 1,459,793 - -0.1 1,459,793 - -1.5 1,459,803 + +0.8 1,459,809 - +1.1 1,459,912 + +0.7 1,459,912 + +0.8 1,459,991 + -0.6 1,459,992 - +0.4 1,460,103 + Pf1N1B4_1279 0.20 +0.5 1,460,104 - Pf1N1B4_1279 0.20 -1.7 1,460,246 + Pf1N1B4_1279 0.36 -0.1 1,460,247 - Pf1N1B4_1279 0.36 -0.7 1,460,247 - Pf1N1B4_1279 0.36 -1.7 1,460,699 + Pf1N1B4_1279 0.87 +0.5 1,460,699 + Pf1N1B4_1279 0.87 -1.6 1,460,699 + Pf1N1B4_1279 0.87 -0.3 1,460,699 + Pf1N1B4_1279 0.87 +0.0 1,460,805 + +0.4 1,460,806 - -0.3 1,460,813 + +1.3
Or see this region's nucleotide sequence