Experiment: 1-ethyl-3-methylimidazolium chloride 80 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1203 and Pf1N1B4_1204 are separated by 41 nucleotides Pf1N1B4_1204 and Pf1N1B4_1205 are separated by 251 nucleotides
Pf1N1B4_1203 - Putative insecticidal toxin complex, at 1,365,611 to 1,368,373
_1203
Pf1N1B4_1204 - Glyoxalase family protein, at 1,368,415 to 1,368,783
_1204
Pf1N1B4_1205 - Sulfate permease, at 1,369,035 to 1,370,420
_1205
Position (kb)
1368
1369 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1367.435 kb on + strand, within Pf1N1B4_1203 at 1367.436 kb on - strand, within Pf1N1B4_1203 at 1367.436 kb on - strand, within Pf1N1B4_1203 at 1367.436 kb on - strand, within Pf1N1B4_1203 at 1367.436 kb on - strand, within Pf1N1B4_1203 at 1367.439 kb on - strand at 1367.439 kb on - strand, within Pf1N1B4_1203 at 1367.439 kb on - strand, within Pf1N1B4_1203 at 1367.441 kb on + strand, within Pf1N1B4_1203 at 1367.442 kb on - strand, within Pf1N1B4_1203 at 1367.454 kb on - strand at 1367.456 kb on + strand, within Pf1N1B4_1203 at 1367.456 kb on + strand, within Pf1N1B4_1203 at 1367.456 kb on + strand, within Pf1N1B4_1203 at 1367.456 kb on + strand, within Pf1N1B4_1203 at 1367.457 kb on - strand, within Pf1N1B4_1203 at 1367.522 kb on + strand, within Pf1N1B4_1203 at 1367.522 kb on + strand, within Pf1N1B4_1203 at 1367.522 kb on + strand, within Pf1N1B4_1203 at 1367.523 kb on - strand at 1367.523 kb on - strand, within Pf1N1B4_1203 at 1367.523 kb on - strand, within Pf1N1B4_1203 at 1367.554 kb on + strand, within Pf1N1B4_1203 at 1367.555 kb on - strand, within Pf1N1B4_1203 at 1367.594 kb on + strand, within Pf1N1B4_1203 at 1367.595 kb on - strand, within Pf1N1B4_1203 at 1367.628 kb on + strand, within Pf1N1B4_1203 at 1367.638 kb on + strand, within Pf1N1B4_1203 at 1367.638 kb on + strand, within Pf1N1B4_1203 at 1367.638 kb on + strand, within Pf1N1B4_1203 at 1367.638 kb on + strand, within Pf1N1B4_1203 at 1367.638 kb on + strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.639 kb on - strand, within Pf1N1B4_1203 at 1367.674 kb on - strand, within Pf1N1B4_1203 at 1367.709 kb on - strand, within Pf1N1B4_1203 at 1367.709 kb on - strand, within Pf1N1B4_1203 at 1367.714 kb on - strand, within Pf1N1B4_1203 at 1367.714 kb on - strand, within Pf1N1B4_1203 at 1367.740 kb on - strand, within Pf1N1B4_1203 at 1367.851 kb on + strand, within Pf1N1B4_1203 at 1367.851 kb on + strand, within Pf1N1B4_1203 at 1367.851 kb on + strand, within Pf1N1B4_1203 at 1367.851 kb on + strand, within Pf1N1B4_1203 at 1367.852 kb on - strand, within Pf1N1B4_1203 at 1367.852 kb on - strand, within Pf1N1B4_1203 at 1367.852 kb on - strand, within Pf1N1B4_1203 at 1367.876 kb on - strand, within Pf1N1B4_1203 at 1367.949 kb on + strand, within Pf1N1B4_1203 at 1367.949 kb on + strand, within Pf1N1B4_1203 at 1367.949 kb on + strand, within Pf1N1B4_1203 at 1367.949 kb on + strand at 1367.949 kb on + strand, within Pf1N1B4_1203 at 1367.950 kb on - strand, within Pf1N1B4_1203 at 1367.950 kb on - strand, within Pf1N1B4_1203 at 1367.950 kb on - strand, within Pf1N1B4_1203 at 1368.002 kb on - strand, within Pf1N1B4_1203 at 1368.002 kb on - strand, within Pf1N1B4_1203 at 1368.014 kb on + strand, within Pf1N1B4_1203 at 1368.028 kb on + strand at 1368.028 kb on + strand, within Pf1N1B4_1203 at 1368.041 kb on - strand, within Pf1N1B4_1203 at 1368.041 kb on - strand, within Pf1N1B4_1203 at 1368.144 kb on + strand at 1368.157 kb on - strand at 1368.165 kb on + strand at 1368.165 kb on + strand at 1368.165 kb on + strand at 1368.166 kb on - strand at 1368.166 kb on - strand at 1368.250 kb on + strand at 1368.250 kb on + strand at 1368.251 kb on - strand at 1368.254 kb on + strand at 1368.254 kb on + strand at 1368.255 kb on - strand at 1368.255 kb on - strand at 1368.255 kb on - strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.265 kb on + strand at 1368.361 kb on + strand at 1368.368 kb on + strand at 1368.368 kb on + strand at 1368.368 kb on + strand at 1368.387 kb on + strand at 1368.423 kb on + strand at 1368.423 kb on + strand at 1368.424 kb on - strand at 1368.424 kb on - strand at 1368.424 kb on - strand at 1368.524 kb on + strand, within Pf1N1B4_1204 at 1368.525 kb on - strand, within Pf1N1B4_1204 at 1368.656 kb on + strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.657 kb on - strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.719 kb on + strand, within Pf1N1B4_1204 at 1368.720 kb on - strand, within Pf1N1B4_1204 at 1368.765 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.817 kb on + strand at 1368.818 kb on - strand at 1368.902 kb on + strand at 1368.902 kb on + strand at 1368.919 kb on + strand at 1368.939 kb on + strand at 1368.939 kb on + strand at 1368.940 kb on - strand at 1369.014 kb on - strand at 1369.102 kb on - strand at 1369.123 kb on + strand at 1369.124 kb on - strand at 1369.163 kb on - strand at 1369.163 kb on - strand at 1369.247 kb on + strand, within Pf1N1B4_1205 at 1369.247 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.318 kb on + strand, within Pf1N1B4_1205 at 1369.319 kb on - strand, within Pf1N1B4_1205 at 1369.406 kb on + strand, within Pf1N1B4_1205 at 1369.580 kb on + strand, within Pf1N1B4_1205 at 1369.581 kb on - strand, within Pf1N1B4_1205 at 1369.581 kb on - strand, within Pf1N1B4_1205
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium chloride 80 mM remove 1,367,435 + Pf1N1B4_1203 0.66 -0.8 1,367,436 - Pf1N1B4_1203 0.66 +1.2 1,367,436 - Pf1N1B4_1203 0.66 -1.1 1,367,436 - Pf1N1B4_1203 0.66 +1.8 1,367,436 - Pf1N1B4_1203 0.66 +0.9 1,367,439 - +0.2 1,367,439 - Pf1N1B4_1203 0.66 -0.1 1,367,439 - Pf1N1B4_1203 0.66 -0.4 1,367,441 + Pf1N1B4_1203 0.66 +0.2 1,367,442 - Pf1N1B4_1203 0.66 +0.5 1,367,454 - +0.7 1,367,456 + Pf1N1B4_1203 0.67 -0.1 1,367,456 + Pf1N1B4_1203 0.67 -1.9 1,367,456 + Pf1N1B4_1203 0.67 +0.4 1,367,456 + Pf1N1B4_1203 0.67 +1.2 1,367,457 - Pf1N1B4_1203 0.67 +0.6 1,367,522 + Pf1N1B4_1203 0.69 +0.9 1,367,522 + Pf1N1B4_1203 0.69 +1.0 1,367,522 + Pf1N1B4_1203 0.69 +0.5 1,367,523 - -1.6 1,367,523 - Pf1N1B4_1203 0.69 -0.3 1,367,523 - Pf1N1B4_1203 0.69 +1.8 1,367,554 + Pf1N1B4_1203 0.70 -0.5 1,367,555 - Pf1N1B4_1203 0.70 +0.2 1,367,594 + Pf1N1B4_1203 0.72 +0.7 1,367,595 - Pf1N1B4_1203 0.72 -1.6 1,367,628 + Pf1N1B4_1203 0.73 +0.2 1,367,638 + Pf1N1B4_1203 0.73 -0.1 1,367,638 + Pf1N1B4_1203 0.73 -1.6 1,367,638 + Pf1N1B4_1203 0.73 +0.7 1,367,638 + Pf1N1B4_1203 0.73 +1.2 1,367,638 + Pf1N1B4_1203 0.73 +0.7 1,367,639 - Pf1N1B4_1203 0.73 -0.6 1,367,639 - Pf1N1B4_1203 0.73 -0.8 1,367,639 - Pf1N1B4_1203 0.73 +1.8 1,367,639 - Pf1N1B4_1203 0.73 -1.3 1,367,639 - Pf1N1B4_1203 0.73 +0.7 1,367,639 - Pf1N1B4_1203 0.73 -0.2 1,367,639 - Pf1N1B4_1203 0.73 -1.5 1,367,639 - Pf1N1B4_1203 0.73 +0.8 1,367,639 - Pf1N1B4_1203 0.73 -1.0 1,367,639 - Pf1N1B4_1203 0.73 +0.7 1,367,674 - Pf1N1B4_1203 0.75 -1.1 1,367,709 - Pf1N1B4_1203 0.76 -0.3 1,367,709 - Pf1N1B4_1203 0.76 +0.4 1,367,714 - Pf1N1B4_1203 0.76 -0.3 1,367,714 - Pf1N1B4_1203 0.76 -1.9 1,367,740 - Pf1N1B4_1203 0.77 +1.2 1,367,851 + Pf1N1B4_1203 0.81 +2.8 1,367,851 + Pf1N1B4_1203 0.81 +1.0 1,367,851 + Pf1N1B4_1203 0.81 -1.6 1,367,851 + Pf1N1B4_1203 0.81 +1.2 1,367,852 - Pf1N1B4_1203 0.81 -0.1 1,367,852 - Pf1N1B4_1203 0.81 +0.2 1,367,852 - Pf1N1B4_1203 0.81 -1.3 1,367,876 - Pf1N1B4_1203 0.82 -0.9 1,367,949 + Pf1N1B4_1203 0.85 -0.8 1,367,949 + Pf1N1B4_1203 0.85 +3.6 1,367,949 + Pf1N1B4_1203 0.85 +0.4 1,367,949 + +0.2 1,367,949 + Pf1N1B4_1203 0.85 -1.3 1,367,950 - Pf1N1B4_1203 0.85 +0.2 1,367,950 - Pf1N1B4_1203 0.85 -0.6 1,367,950 - Pf1N1B4_1203 0.85 +0.8 1,368,002 - Pf1N1B4_1203 0.87 -0.1 1,368,002 - Pf1N1B4_1203 0.87 +1.2 1,368,014 + Pf1N1B4_1203 0.87 -0.5 1,368,028 + +2.2 1,368,028 + Pf1N1B4_1203 0.87 -1.8 1,368,041 - Pf1N1B4_1203 0.88 +1.8 1,368,041 - Pf1N1B4_1203 0.88 +0.9 1,368,144 + +0.5 1,368,157 - +1.7 1,368,165 + +0.1 1,368,165 + -0.8 1,368,165 + -1.1 1,368,166 - -0.3 1,368,166 - +0.2 1,368,250 + +0.5 1,368,250 + +0.8 1,368,251 - +0.4 1,368,254 + +0.2 1,368,254 + -0.9 1,368,255 - -1.3 1,368,255 - -0.3 1,368,255 - +0.2 1,368,265 + -1.3 1,368,265 + +0.0 1,368,265 + -1.6 1,368,265 + -0.3 1,368,361 + +2.2 1,368,368 + +0.2 1,368,368 + -0.9 1,368,368 + -0.1 1,368,387 + -1.1 1,368,423 + -0.5 1,368,423 + -0.9 1,368,424 - +0.2 1,368,424 - +0.4 1,368,424 - -0.5 1,368,524 + Pf1N1B4_1204 0.30 +1.0 1,368,525 - Pf1N1B4_1204 0.30 -0.8 1,368,656 + Pf1N1B4_1204 0.65 -1.1 1,368,657 - Pf1N1B4_1204 0.66 -0.9 1,368,657 - Pf1N1B4_1204 0.66 +0.9 1,368,657 - Pf1N1B4_1204 0.66 +0.6 1,368,657 - Pf1N1B4_1204 0.66 -2.7 1,368,657 - Pf1N1B4_1204 0.66 +1.8 1,368,657 - Pf1N1B4_1204 0.66 +0.5 1,368,719 + Pf1N1B4_1204 0.82 +0.6 1,368,719 + Pf1N1B4_1204 0.82 -0.1 1,368,719 + Pf1N1B4_1204 0.82 -0.2 1,368,720 - Pf1N1B4_1204 0.83 +0.5 1,368,765 + -1.2 1,368,817 + +0.7 1,368,817 + +0.7 1,368,817 + +0.8 1,368,817 + -1.3 1,368,817 + +0.3 1,368,817 + -0.6 1,368,818 - +0.2 1,368,902 + +0.6 1,368,902 + +0.5 1,368,919 + +0.7 1,368,939 + -2.1 1,368,939 + +0.2 1,368,940 - +0.7 1,369,014 - +1.8 1,369,102 - +0.6 1,369,123 + -0.3 1,369,124 - -0.0 1,369,163 - -0.2 1,369,163 - +2.2 1,369,247 + Pf1N1B4_1205 0.15 +0.2 1,369,247 + Pf1N1B4_1205 0.15 +0.2 1,369,318 + Pf1N1B4_1205 0.20 +0.4 1,369,318 + Pf1N1B4_1205 0.20 -1.1 1,369,318 + Pf1N1B4_1205 0.20 -0.3 1,369,318 + Pf1N1B4_1205 0.20 -1.7 1,369,319 - Pf1N1B4_1205 0.20 +1.7 1,369,406 + Pf1N1B4_1205 0.27 -1.9 1,369,580 + Pf1N1B4_1205 0.39 -0.3 1,369,581 - Pf1N1B4_1205 0.39 -0.8 1,369,581 - Pf1N1B4_1205 0.39 -0.2
Or see this region's nucleotide sequence