Experiment: soil sample 8; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABZR87_RS11750 and ABZR87_RS11755 are separated by 37 nucleotides ABZR87_RS11755 and ABZR87_RS11760 are separated by 14 nucleotides ABZR87_RS11760 and ABZR87_RS11765 are separated by 60 nucleotides ABZR87_RS11765 and ABZR87_RS11770 are separated by 45 nucleotides
ABZR87_RS11750: ABZR87_RS11750 - succinyl-diaminopimelate desuccinylase, at 2,490,679 to 2,491,830
_RS11750
ABZR87_RS11755: ABZR87_RS11755 - arsenate reductase, at 2,491,868 to 2,492,212
_RS11755
ABZR87_RS11760: ABZR87_RS11760 - membrane protein, at 2,492,227 to 2,492,640
_RS11760
ABZR87_RS11765: ABZR87_RS11765 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 2,492,701 to 2,493,528
_RS11765
ABZR87_RS11770: ABZR87_RS11770 - succinyldiaminopimelate transaminase, at 2,493,574 to 2,494,773
_RS11770
Position (kb) 2492
2493 Strain fitness (log2 ratio) -2
-1
0
1 at 2491.870 kb on - strand at 2491.885 kb on - strand at 2492.133 kb on + strand, within ABZR87_RS11755 at 2492.134 kb on - strand, within ABZR87_RS11755 at 2492.436 kb on + strand, within ABZR87_RS11760 at 2492.676 kb on + strand at 2492.676 kb on + strand at 2492.703 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction soil sample 8; outgrowth in LB remove 2,491,870 - +1.2 2,491,885 - -2.1 2,492,133 + ABZR87_RS11755 0.77 -1.1 2,492,134 - ABZR87_RS11755 0.77 -0.0 2,492,436 + ABZR87_RS11760 0.50 -1.0 2,492,676 + +0.0 2,492,676 + -1.1 2,492,703 - -0.4
Or see this region's nucleotide sequence