Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5883 and Pf1N1B4_5884 are separated by 380 nucleotides Pf1N1B4_5884 and Pf1N1B4_5885 overlap by 4 nucleotides
Pf1N1B4_5883 - FIG00960892: hypothetical protein, at 325,237 to 326,934
_5883
Pf1N1B4_5884 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 327,315 to 328,667
_5884
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1
2 at 326.393 kb on + strand, within Pf1N1B4_5883 at 326.394 kb on - strand at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.426 kb on + strand, within Pf1N1B4_5883 at 326.427 kb on - strand, within Pf1N1B4_5883 at 326.676 kb on - strand at 326.676 kb on - strand, within Pf1N1B4_5883 at 326.836 kb on - strand at 326.883 kb on - strand at 326.897 kb on + strand at 326.898 kb on - strand at 327.068 kb on + strand at 327.286 kb on - strand at 327.328 kb on + strand at 327.342 kb on + strand at 327.355 kb on + strand at 327.356 kb on - strand at 327.879 kb on + strand, within Pf1N1B4_5884 at 327.880 kb on - strand at 328.121 kb on + strand, within Pf1N1B4_5884 at 328.266 kb on - strand, within Pf1N1B4_5884 at 328.381 kb on - strand, within Pf1N1B4_5884 at 328.417 kb on - strand, within Pf1N1B4_5884 at 328.597 kb on + strand at 328.597 kb on + strand at 328.598 kb on - strand at 328.671 kb on + strand at 328.671 kb on + strand at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 326,393 + Pf1N1B4_5883 0.68 -1.0 326,394 - -1.7 326,426 + Pf1N1B4_5883 0.70 +1.6 326,426 + Pf1N1B4_5883 0.70 +0.6 326,426 + Pf1N1B4_5883 0.70 -0.0 326,427 - Pf1N1B4_5883 0.70 +0.3 326,676 - +0.7 326,676 - Pf1N1B4_5883 0.85 +1.6 326,836 - +1.3 326,883 - -0.2 326,897 + +0.8 326,898 - -1.0 327,068 + -1.3 327,286 - +0.3 327,328 + -0.9 327,342 + -0.4 327,355 + +0.3 327,356 - +1.0 327,879 + Pf1N1B4_5884 0.42 -1.7 327,880 - -1.7 328,121 + Pf1N1B4_5884 0.60 -1.3 328,266 - Pf1N1B4_5884 0.70 -0.3 328,381 - Pf1N1B4_5884 0.79 -0.0 328,417 - Pf1N1B4_5884 0.81 -0.8 328,597 + -0.7 328,597 + +0.1 328,598 - -0.0 328,671 + -0.7 328,671 + +0.7 328,744 - +0.1 328,773 - Pf1N1B4_5885 0.10 +1.8 328,773 - Pf1N1B4_5885 0.10 +0.7 328,938 - Pf1N1B4_5885 0.26 +0.1 328,962 - Pf1N1B4_5885 0.28 -0.2 329,036 + Pf1N1B4_5885 0.35 +0.7 329,036 + +0.3 329,036 + Pf1N1B4_5885 0.35 -0.3 329,121 - Pf1N1B4_5885 0.43 -2.1 329,162 + -2.3 329,162 + Pf1N1B4_5885 0.47 +0.0 329,162 + Pf1N1B4_5885 0.47 -1.0 329,163 - Pf1N1B4_5885 0.47 -0.5 329,163 - Pf1N1B4_5885 0.47 -0.4 329,324 + Pf1N1B4_5885 0.62 +0.4 329,324 + Pf1N1B4_5885 0.62 +1.7 329,325 - Pf1N1B4_5885 0.62 -0.7 329,325 - Pf1N1B4_5885 0.62 -1.6 329,442 - Pf1N1B4_5885 0.73 +1.0 329,442 - Pf1N1B4_5885 0.73 +1.6 329,481 - Pf1N1B4_5885 0.77 +1.3 329,660 + +1.3 329,661 - -0.2 329,663 + +0.3
Or see this region's nucleotide sequence