Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5344 and Pf1N1B4_5345 overlap by 4 nucleotides Pf1N1B4_5345 and Pf1N1B4_5346 are separated by 80 nucleotides Pf1N1B4_5346 and Pf1N1B4_5347 are separated by 136 nucleotides Pf1N1B4_5347 and Pf1N1B4_5348 are separated by 101 nucleotides
Pf1N1B4_5344 - hypothetical protein, at 86,092 to 86,823
_5344
Pf1N1B4_5345 - hypothetical protein, at 86,820 to 87,074
_5345
Pf1N1B4_5346 - hypothetical protein, at 87,155 to 87,868
_5346
Pf1N1B4_5347 - hypothetical protein, at 88,005 to 88,352
_5347
Pf1N1B4_5348 - hypothetical protein, at 88,454 to 88,576
_5348
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.352 kb on + strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.353 kb on - strand, within Pf1N1B4_5344 at 86.369 kb on + strand at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.369 kb on + strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.370 kb on - strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.444 kb on + strand, within Pf1N1B4_5344 at 86.445 kb on - strand, within Pf1N1B4_5344 at 86.445 kb on - strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.557 kb on + strand, within Pf1N1B4_5344 at 86.558 kb on - strand, within Pf1N1B4_5344 at 86.571 kb on + strand, within Pf1N1B4_5344 at 86.572 kb on - strand at 86.658 kb on - strand, within Pf1N1B4_5344 at 86.696 kb on + strand, within Pf1N1B4_5344 at 86.737 kb on + strand, within Pf1N1B4_5344 at 86.827 kb on + strand at 86.827 kb on + strand at 86.909 kb on - strand, within Pf1N1B4_5345 at 87.088 kb on + strand at 87.156 kb on + strand at 87.156 kb on + strand at 87.156 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.207 kb on + strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.208 kb on - strand at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand, within Pf1N1B4_5346 at 87.401 kb on - strand at 87.401 kb on - strand at 87.597 kb on + strand, within Pf1N1B4_5346 at 87.835 kb on + strand at 87.914 kb on + strand at 87.914 kb on + strand at 87.914 kb on + strand at 87.915 kb on - strand at 87.920 kb on + strand at 87.986 kb on - strand at 88.016 kb on - strand at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.495 kb on - strand, within Pf1N1B4_5348 at 88.560 kb on - strand at 88.560 kb on - strand, within Pf1N1B4_5348 at 88.642 kb on - strand at 88.671 kb on + strand at 88.672 kb on - strand at 88.793 kb on - strand at 88.803 kb on + strand at 88.804 kb on - strand at 88.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 86,352 + Pf1N1B4_5344 0.36 +1.0 86,352 + Pf1N1B4_5344 0.36 +0.1 86,352 + Pf1N1B4_5344 0.36 -0.3 86,353 - Pf1N1B4_5344 0.36 -0.3 86,353 - Pf1N1B4_5344 0.36 +0.7 86,353 - Pf1N1B4_5344 0.36 -0.3 86,353 - Pf1N1B4_5344 0.36 -1.6 86,369 + +0.1 86,369 + Pf1N1B4_5344 0.38 -0.1 86,369 + Pf1N1B4_5344 0.38 +0.5 86,369 + Pf1N1B4_5344 0.38 +1.7 86,370 - Pf1N1B4_5344 0.38 -0.3 86,370 - Pf1N1B4_5344 0.38 -1.3 86,370 - Pf1N1B4_5344 0.38 -0.0 86,370 - Pf1N1B4_5344 0.38 +0.7 86,444 + Pf1N1B4_5344 0.48 +0.7 86,444 + Pf1N1B4_5344 0.48 +0.8 86,444 + Pf1N1B4_5344 0.48 +0.7 86,445 - Pf1N1B4_5344 0.48 +0.3 86,445 - Pf1N1B4_5344 0.48 -2.5 86,557 + Pf1N1B4_5344 0.64 +0.7 86,557 + Pf1N1B4_5344 0.64 -2.0 86,557 + Pf1N1B4_5344 0.64 -1.3 86,558 - Pf1N1B4_5344 0.64 +0.9 86,571 + Pf1N1B4_5344 0.65 -0.6 86,572 - +0.1 86,658 - Pf1N1B4_5344 0.77 -2.5 86,696 + Pf1N1B4_5344 0.83 +1.2 86,737 + Pf1N1B4_5344 0.88 +1.5 86,827 + -0.0 86,827 + -0.0 86,909 - Pf1N1B4_5345 0.35 -3.5 87,088 + +0.9 87,156 + -0.5 87,156 + +0.3 87,156 + -0.9 87,207 + -1.1 87,207 + -1.6 87,207 + -0.5 87,207 + +0.4 87,208 - -1.0 87,208 - +0.1 87,208 - -0.1 87,208 - +1.3 87,208 - -0.9 87,208 - -1.3 87,208 - +1.7 87,401 - Pf1N1B4_5346 0.34 +0.2 87,401 - Pf1N1B4_5346 0.34 -0.6 87,401 - Pf1N1B4_5346 0.34 -1.0 87,401 - Pf1N1B4_5346 0.34 -1.3 87,401 - Pf1N1B4_5346 0.34 -1.1 87,401 - +1.1 87,401 - Pf1N1B4_5346 0.34 +0.0 87,401 - Pf1N1B4_5346 0.34 +0.1 87,401 - +0.7 87,401 - +0.7 87,597 + Pf1N1B4_5346 0.62 -1.6 87,835 + -0.3 87,914 + +0.5 87,914 + -0.5 87,914 + -1.9 87,915 - -0.0 87,920 + +0.1 87,986 - +1.3 88,016 - -0.3 88,495 - Pf1N1B4_5348 0.33 -1.1 88,495 - Pf1N1B4_5348 0.33 -0.9 88,495 - Pf1N1B4_5348 0.33 -0.3 88,560 - +0.1 88,560 - Pf1N1B4_5348 0.86 -0.2 88,642 - +1.1 88,671 + +1.3 88,672 - +1.1 88,793 - -0.9 88,803 + -0.1 88,804 - -0.2 88,859 - -0.0
Or see this region's nucleotide sequence