Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_464 and Pf1N1B4_465 overlap by 4 nucleotides Pf1N1B4_465 and Pf1N1B4_466 are separated by 232 nucleotides
Pf1N1B4_464 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 555,443 to 557,764
_464
Pf1N1B4_465 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 557,761 to 558,234
_465
Pf1N1B4_466 - UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158), at 558,467 to 559,486
_466
Position (kb) 557
558
559 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 556.818 kb on + strand, within Pf1N1B4_464 at 556.818 kb on + strand, within Pf1N1B4_464 at 556.819 kb on - strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 556.885 kb on + strand, within Pf1N1B4_464 at 557.083 kb on - strand, within Pf1N1B4_464 at 557.266 kb on + strand, within Pf1N1B4_464 at 557.266 kb on + strand, within Pf1N1B4_464 at 557.370 kb on + strand, within Pf1N1B4_464 at 557.574 kb on + strand at 557.574 kb on + strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.575 kb on - strand at 557.582 kb on - strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand at 557.822 kb on + strand, within Pf1N1B4_465 at 557.822 kb on + strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 557.823 kb on - strand, within Pf1N1B4_465 at 558.013 kb on + strand at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.013 kb on + strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.014 kb on - strand, within Pf1N1B4_465 at 558.019 kb on - strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.093 kb on + strand, within Pf1N1B4_465 at 558.190 kb on + strand at 558.191 kb on - strand at 558.203 kb on + strand at 558.203 kb on + strand at 558.204 kb on - strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.228 kb on + strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.229 kb on - strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.248 kb on + strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.249 kb on - strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.252 kb on + strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.253 kb on - strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.254 kb on + strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.255 kb on - strand at 558.326 kb on - strand at 558.388 kb on + strand at 558.393 kb on + strand at 558.393 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 556,818 + Pf1N1B4_464 0.59 -0.8 556,818 + Pf1N1B4_464 0.59 -0.5 556,819 - Pf1N1B4_464 0.59 -0.7 556,885 + Pf1N1B4_464 0.62 -0.8 556,885 + Pf1N1B4_464 0.62 +0.8 556,885 + Pf1N1B4_464 0.62 -0.3 557,083 - Pf1N1B4_464 0.71 -0.6 557,266 + Pf1N1B4_464 0.79 -1.5 557,266 + Pf1N1B4_464 0.79 +0.5 557,370 + Pf1N1B4_464 0.83 +0.3 557,574 + +0.3 557,574 + -0.7 557,575 - -2.3 557,575 - -1.9 557,575 - +0.2 557,582 - -2.1 557,822 + Pf1N1B4_465 0.13 +1.2 557,822 + Pf1N1B4_465 0.13 -0.1 557,822 + +0.5 557,822 + Pf1N1B4_465 0.13 -0.1 557,822 + Pf1N1B4_465 0.13 -2.1 557,823 - Pf1N1B4_465 0.13 -2.3 557,823 - Pf1N1B4_465 0.13 +0.5 558,013 + +1.5 558,013 + Pf1N1B4_465 0.53 -0.5 558,013 + Pf1N1B4_465 0.53 -1.5 558,013 + Pf1N1B4_465 0.53 -0.2 558,013 + Pf1N1B4_465 0.53 -2.0 558,013 + Pf1N1B4_465 0.53 -2.3 558,013 + Pf1N1B4_465 0.53 -0.5 558,014 - Pf1N1B4_465 0.53 +0.2 558,014 - Pf1N1B4_465 0.53 -0.8 558,014 - Pf1N1B4_465 0.53 +0.3 558,014 - +1.1 558,014 - Pf1N1B4_465 0.53 +3.2 558,014 - Pf1N1B4_465 0.53 -0.4 558,014 - Pf1N1B4_465 0.53 -0.5 558,014 - Pf1N1B4_465 0.53 +0.8 558,014 - Pf1N1B4_465 0.53 +0.1 558,019 - Pf1N1B4_465 0.54 -0.7 558,093 + Pf1N1B4_465 0.70 -3.5 558,093 + Pf1N1B4_465 0.70 -0.9 558,190 + +0.2 558,191 - -0.2 558,203 + +1.9 558,203 + -0.8 558,204 - +0.8 558,228 + +1.5 558,228 + -0.8 558,228 + +0.5 558,228 + -2.3 558,228 + -1.3 558,229 - -0.8 558,229 - +0.2 558,229 - +0.5 558,248 + +1.8 558,248 + +0.2 558,248 + -0.4 558,248 + -0.5 558,248 + -0.8 558,248 + -0.2 558,249 - -1.5 558,249 - +0.4 558,249 - +1.1 558,252 + -0.6 558,252 + -0.6 558,252 + -0.5 558,252 + -1.1 558,252 + -1.1 558,252 + -1.8 558,252 + -1.2 558,252 + +0.3 558,253 - -2.3 558,253 - -1.3 558,253 - +1.5 558,253 - -0.8 558,253 - +0.5 558,254 + +0.2 558,254 + -0.8 558,254 + -1.8 558,254 + -0.5 558,254 + -0.1 558,254 + -0.3 558,254 + +0.1 558,255 - +0.2 558,255 - -1.5 558,255 - -1.1 558,326 - -1.1 558,388 + +0.5 558,393 + -2.1 558,393 + -1.3
Or see this region's nucleotide sequence