Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4517 and Pf1N1B4_4518 are separated by 622 nucleotides Pf1N1B4_4518 and Pf1N1B4_4519 are separated by 129 nucleotides
Pf1N1B4_4517 - Dihydropyrimidinase (EC 3.5.2.2), at 4,979,635 to 4,981,074
_4517
Pf1N1B4_4518 - Possible pyrimidine permease in reductive pathway, at 4,981,697 to 4,983,181
_4518
Pf1N1B4_4519 - Beta-ureidopropionase (EC 3.5.1.6), at 4,983,311 to 4,984,594
_4519
Position (kb)
4981
4982
4983
4984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4980.716 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.891 kb on + strand, within Pf1N1B4_4517 at 4980.892 kb on - strand at 4981.010 kb on + strand at 4981.025 kb on + strand at 4981.042 kb on - strand at 4981.115 kb on + strand at 4981.125 kb on - strand at 4981.149 kb on + strand at 4981.782 kb on - strand at 4981.880 kb on + strand, within Pf1N1B4_4518 at 4981.881 kb on - strand, within Pf1N1B4_4518 at 4982.065 kb on - strand, within Pf1N1B4_4518 at 4982.400 kb on - strand, within Pf1N1B4_4518 at 4982.748 kb on - strand, within Pf1N1B4_4518 at 4982.748 kb on - strand at 4982.847 kb on + strand, within Pf1N1B4_4518 at 4982.865 kb on - strand, within Pf1N1B4_4518 at 4983.284 kb on - strand at 4983.284 kb on - strand at 4984.146 kb on - strand, within Pf1N1B4_4519
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,980,716 + Pf1N1B4_4517 0.75 +0.1 4,980,891 + Pf1N1B4_4517 0.87 +0.4 4,980,891 + Pf1N1B4_4517 0.87 +0.5 4,980,891 + Pf1N1B4_4517 0.87 -0.6 4,980,891 + Pf1N1B4_4517 0.87 -0.3 4,980,892 - +2.7 4,981,010 + +0.5 4,981,025 + -0.3 4,981,042 - -1.5 4,981,115 + +0.3 4,981,125 - -0.9 4,981,149 + +0.2 4,981,782 - -1.2 4,981,880 + Pf1N1B4_4518 0.12 -1.1 4,981,881 - Pf1N1B4_4518 0.12 -2.9 4,982,065 - Pf1N1B4_4518 0.25 -2.1 4,982,400 - Pf1N1B4_4518 0.47 +1.1 4,982,748 - Pf1N1B4_4518 0.71 -0.3 4,982,748 - +0.1 4,982,847 + Pf1N1B4_4518 0.77 -0.9 4,982,865 - Pf1N1B4_4518 0.79 -0.3 4,983,284 - -0.3 4,983,284 - -1.2 4,984,146 - Pf1N1B4_4519 0.65 +0.1
Or see this region's nucleotide sequence