Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4237 and Pf1N1B4_4238 are separated by 260 nucleotides Pf1N1B4_4238 and Pf1N1B4_4239 are separated by 54 nucleotides Pf1N1B4_4239 and Pf1N1B4_4240 are separated by 137 nucleotides
Pf1N1B4_4237 - COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like, at 4,696,065 to 4,697,528
_4237
Pf1N1B4_4238 - hypothetical protein, at 4,697,789 to 4,698,370
_4238
Pf1N1B4_4239 - hypothetical protein, at 4,698,425 to 4,698,976
_4239
Pf1N1B4_4240 - hypothetical protein, at 4,699,114 to 4,699,263
_4240
Position (kb) 4697
4698
4699 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4696.804 kb on + strand, within Pf1N1B4_4237 at 4696.805 kb on - strand at 4696.814 kb on + strand, within Pf1N1B4_4237 at 4696.815 kb on - strand, within Pf1N1B4_4237 at 4697.217 kb on - strand, within Pf1N1B4_4237 at 4697.232 kb on + strand, within Pf1N1B4_4237 at 4697.232 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.242 kb on + strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.243 kb on - strand, within Pf1N1B4_4237 at 4697.296 kb on + strand, within Pf1N1B4_4237 at 4697.476 kb on + strand at 4697.613 kb on + strand at 4698.019 kb on - strand, within Pf1N1B4_4238 at 4698.043 kb on - strand, within Pf1N1B4_4238 at 4698.057 kb on + strand, within Pf1N1B4_4238 at 4698.058 kb on - strand at 4698.196 kb on - strand, within Pf1N1B4_4238 at 4698.196 kb on - strand, within Pf1N1B4_4238 at 4698.306 kb on - strand, within Pf1N1B4_4238 at 4698.376 kb on + strand at 4698.384 kb on + strand at 4698.384 kb on + strand at 4698.384 kb on + strand at 4698.385 kb on - strand at 4698.726 kb on + strand, within Pf1N1B4_4239 at 4698.727 kb on - strand, within Pf1N1B4_4239 at 4698.732 kb on + strand at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.733 kb on - strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand, within Pf1N1B4_4239 at 4698.873 kb on + strand at 4698.874 kb on - strand, within Pf1N1B4_4239 at 4698.880 kb on - strand, within Pf1N1B4_4239 at 4699.076 kb on + strand at 4699.109 kb on - strand at 4699.115 kb on + strand at 4699.165 kb on - strand, within Pf1N1B4_4240 at 4699.168 kb on + strand, within Pf1N1B4_4240 at 4699.169 kb on - strand, within Pf1N1B4_4240 at 4699.281 kb on + strand at 4699.282 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,696,804 + Pf1N1B4_4237 0.50 -0.7 4,696,805 - -0.7 4,696,814 + Pf1N1B4_4237 0.51 -0.6 4,696,815 - Pf1N1B4_4237 0.51 +0.4 4,697,217 - Pf1N1B4_4237 0.79 -1.0 4,697,232 + Pf1N1B4_4237 0.80 +0.1 4,697,232 + Pf1N1B4_4237 0.80 -0.4 4,697,242 + Pf1N1B4_4237 0.80 -1.3 4,697,242 + Pf1N1B4_4237 0.80 -0.5 4,697,242 + Pf1N1B4_4237 0.80 +1.5 4,697,242 + Pf1N1B4_4237 0.80 +0.1 4,697,242 + Pf1N1B4_4237 0.80 -0.2 4,697,243 - Pf1N1B4_4237 0.80 +1.7 4,697,243 - Pf1N1B4_4237 0.80 -2.5 4,697,243 - Pf1N1B4_4237 0.80 -1.1 4,697,296 + Pf1N1B4_4237 0.84 -0.2 4,697,476 + +0.3 4,697,613 + -1.1 4,698,019 - Pf1N1B4_4238 0.40 -0.6 4,698,043 - Pf1N1B4_4238 0.44 -1.1 4,698,057 + Pf1N1B4_4238 0.46 -1.3 4,698,058 - -0.7 4,698,196 - Pf1N1B4_4238 0.70 -2.1 4,698,196 - Pf1N1B4_4238 0.70 +0.3 4,698,306 - Pf1N1B4_4238 0.89 -1.2 4,698,376 + +1.0 4,698,384 + -0.1 4,698,384 + -1.7 4,698,384 + +0.3 4,698,385 - -1.5 4,698,726 + Pf1N1B4_4239 0.55 +0.1 4,698,727 - Pf1N1B4_4239 0.55 -0.3 4,698,732 + +1.3 4,698,733 - Pf1N1B4_4239 0.56 -1.9 4,698,733 - Pf1N1B4_4239 0.56 -1.1 4,698,873 + Pf1N1B4_4239 0.81 -0.2 4,698,873 + Pf1N1B4_4239 0.81 +0.7 4,698,873 + +0.3 4,698,874 - Pf1N1B4_4239 0.81 +2.6 4,698,880 - Pf1N1B4_4239 0.82 -0.3 4,699,076 + -0.7 4,699,109 - +0.7 4,699,115 + +0.8 4,699,165 - Pf1N1B4_4240 0.34 +0.5 4,699,168 + Pf1N1B4_4240 0.36 +1.9 4,699,169 - Pf1N1B4_4240 0.37 -0.4 4,699,281 + -0.2 4,699,282 - -0.7
Or see this region's nucleotide sequence