Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4016 and Pf1N1B4_4017 overlap by 4 nucleotides Pf1N1B4_4017 and Pf1N1B4_4018 are separated by 467 nucleotides
Pf1N1B4_4016 - Heavy metal sensor histidine kinase, at 4,466,486 to 4,467,889
_4016
Pf1N1B4_4017 - DNA-binding heavy metal response regulator, at 4,467,886 to 4,468,560
_4017
Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287
_4018
Position (kb)
4467
4468
4469 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4466.979 kb on - strand at 4467.052 kb on - strand at 4467.614 kb on + strand, within Pf1N1B4_4016 at 4467.615 kb on - strand, within Pf1N1B4_4016 at 4467.630 kb on + strand, within Pf1N1B4_4016 at 4467.802 kb on - strand at 4467.845 kb on - strand at 4467.908 kb on + strand at 4468.272 kb on - strand, within Pf1N1B4_4017 at 4468.272 kb on - strand, within Pf1N1B4_4017 at 4468.415 kb on - strand, within Pf1N1B4_4017 at 4468.427 kb on + strand, within Pf1N1B4_4017 at 4468.428 kb on - strand, within Pf1N1B4_4017 at 4468.486 kb on - strand, within Pf1N1B4_4017 at 4468.513 kb on + strand at 4468.514 kb on - strand at 4468.514 kb on - strand at 4468.593 kb on - strand at 4468.823 kb on + strand at 4468.824 kb on - strand at 4468.849 kb on + strand at 4468.854 kb on - strand at 4468.874 kb on + strand at 4468.875 kb on - strand at 4468.875 kb on - strand at 4468.941 kb on + strand at 4469.038 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,466,979 - -1.3 4,467,052 - -0.7 4,467,614 + Pf1N1B4_4016 0.80 -2.3 4,467,615 - Pf1N1B4_4016 0.80 -0.5 4,467,630 + Pf1N1B4_4016 0.81 +1.5 4,467,802 - +1.9 4,467,845 - +0.9 4,467,908 + +0.3 4,468,272 - Pf1N1B4_4017 0.57 -1.3 4,468,272 - Pf1N1B4_4017 0.57 -1.7 4,468,415 - Pf1N1B4_4017 0.78 -1.1 4,468,427 + Pf1N1B4_4017 0.80 +0.7 4,468,428 - Pf1N1B4_4017 0.80 -0.7 4,468,486 - Pf1N1B4_4017 0.89 -0.5 4,468,513 + +0.5 4,468,514 - +2.3 4,468,514 - -1.3 4,468,593 - +1.5 4,468,823 + +0.3 4,468,824 - -0.4 4,468,849 + -0.5 4,468,854 - +1.3 4,468,874 + +0.3 4,468,875 - -1.9 4,468,875 - +0.0 4,468,941 + +0.5 4,469,038 + -0.0
Or see this region's nucleotide sequence