Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4006 and Pf1N1B4_4007 are separated by 111 nucleotides Pf1N1B4_4007 and Pf1N1B4_4008 are separated by 367 nucleotides
Pf1N1B4_4006 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 4,455,762 to 4,457,144
_4006
Pf1N1B4_4007 - sigma-54 dependent transcriptional regulator, at 4,457,256 to 4,459,106
_4007
Pf1N1B4_4008 - Various polyols ABC transporter, ATP-binding component, at 4,459,474 to 4,460,568
_4008
Position (kb)
4457
4458
4459
4460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.690 kb on - strand at 4457.690 kb on - strand, within Pf1N1B4_4007 at 4457.959 kb on - strand, within Pf1N1B4_4007 at 4457.959 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4459.133 kb on - strand at 4459.213 kb on - strand at 4459.219 kb on - strand at 4459.352 kb on + strand at 4459.353 kb on - strand at 4459.459 kb on + strand at 4459.711 kb on + strand, within Pf1N1B4_4008 at 4459.712 kb on - strand, within Pf1N1B4_4008 at 4459.966 kb on + strand, within Pf1N1B4_4008 at 4460.032 kb on - strand, within Pf1N1B4_4008 at 4460.035 kb on + strand, within Pf1N1B4_4008 at 4460.036 kb on - strand, within Pf1N1B4_4008 at 4460.036 kb on - strand, within Pf1N1B4_4008 at 4460.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,457,285 - -1.3 4,457,285 - -1.3 4,457,285 - -3.1 4,457,285 - -0.7 4,457,690 - +0.9 4,457,690 - Pf1N1B4_4007 0.23 -0.9 4,457,959 - Pf1N1B4_4007 0.38 -2.0 4,457,959 - Pf1N1B4_4007 0.38 -0.2 4,458,605 - Pf1N1B4_4007 0.73 -1.3 4,458,605 - Pf1N1B4_4007 0.73 -1.3 4,458,605 - Pf1N1B4_4007 0.73 -1.0 4,458,605 - Pf1N1B4_4007 0.73 -0.2 4,459,133 - -0.4 4,459,213 - -1.6 4,459,219 - +0.3 4,459,352 + -0.4 4,459,353 - -2.0 4,459,459 + +2.2 4,459,711 + Pf1N1B4_4008 0.22 -0.1 4,459,712 - Pf1N1B4_4008 0.22 -0.7 4,459,966 + Pf1N1B4_4008 0.45 -0.4 4,460,032 - Pf1N1B4_4008 0.51 +0.1 4,460,035 + Pf1N1B4_4008 0.51 +1.3 4,460,036 - Pf1N1B4_4008 0.51 -2.0 4,460,036 - Pf1N1B4_4008 0.51 +1.3 4,460,036 - -1.7
Or see this region's nucleotide sequence