Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3981 and Pf1N1B4_3982 are separated by 483 nucleotides Pf1N1B4_3982 and Pf1N1B4_3983 are separated by 120 nucleotides Pf1N1B4_3983 and Pf1N1B4_3984 are separated by 39 nucleotides
Pf1N1B4_3981 - Predicted carboxypeptidase, at 4,422,063 to 4,423,214
_3981
Pf1N1B4_3982 - FIG00953347: hypothetical protein, at 4,423,698 to 4,424,039
_3982
Pf1N1B4_3983 - cI, at 4,424,160 to 4,424,834
_3983
Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 4,424,874 to 4,426,829
_3984
Position (kb)
4424
4425 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4423.202 kb on + strand at 4423.203 kb on - strand at 4423.332 kb on - strand at 4423.371 kb on - strand at 4423.411 kb on - strand at 4423.466 kb on - strand at 4423.466 kb on - strand at 4423.488 kb on - strand at 4423.488 kb on - strand at 4423.488 kb on - strand at 4423.488 kb on - strand at 4423.552 kb on - strand at 4423.552 kb on - strand at 4423.570 kb on - strand at 4423.570 kb on - strand at 4423.570 kb on - strand at 4423.570 kb on - strand at 4423.570 kb on - strand at 4423.570 kb on - strand at 4423.681 kb on - strand at 4423.681 kb on - strand at 4423.681 kb on - strand at 4423.691 kb on - strand at 4423.842 kb on + strand, within Pf1N1B4_3982 at 4423.843 kb on - strand, within Pf1N1B4_3982 at 4423.843 kb on - strand, within Pf1N1B4_3982 at 4423.942 kb on + strand, within Pf1N1B4_3982 at 4423.942 kb on + strand, within Pf1N1B4_3982 at 4423.943 kb on - strand, within Pf1N1B4_3982 at 4423.943 kb on - strand at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.945 kb on + strand, within Pf1N1B4_3982 at 4423.946 kb on - strand, within Pf1N1B4_3982 at 4423.946 kb on - strand, within Pf1N1B4_3982 at 4424.060 kb on + strand at 4424.060 kb on + strand at 4424.061 kb on - strand at 4424.085 kb on + strand at 4424.085 kb on + strand at 4424.086 kb on - strand at 4424.088 kb on + strand at 4424.088 kb on + strand at 4424.088 kb on + strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.089 kb on - strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.092 kb on + strand at 4424.093 kb on - strand at 4424.093 kb on - strand at 4424.093 kb on - strand at 4424.098 kb on - strand at 4424.121 kb on + strand at 4424.121 kb on + strand at 4424.156 kb on + strand at 4424.231 kb on - strand at 4424.231 kb on - strand, within Pf1N1B4_3983 at 4424.282 kb on + strand, within Pf1N1B4_3983 at 4424.282 kb on + strand at 4424.344 kb on - strand, within Pf1N1B4_3983 at 4424.344 kb on - strand, within Pf1N1B4_3983 at 4424.415 kb on + strand, within Pf1N1B4_3983 at 4424.415 kb on + strand, within Pf1N1B4_3983 at 4424.416 kb on - strand at 4424.482 kb on + strand, within Pf1N1B4_3983 at 4424.538 kb on + strand, within Pf1N1B4_3983 at 4424.807 kb on - strand at 4424.833 kb on - strand at 4424.935 kb on + strand at 4424.936 kb on - strand at 4424.936 kb on - strand at 4425.130 kb on + strand, within Pf1N1B4_3984 at 4425.131 kb on - strand, within Pf1N1B4_3984 at 4425.131 kb on - strand, within Pf1N1B4_3984 at 4425.169 kb on + strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.170 kb on - strand, within Pf1N1B4_3984 at 4425.236 kb on - strand, within Pf1N1B4_3984 at 4425.671 kb on - strand, within Pf1N1B4_3984
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,423,202 + +0.3 4,423,203 - +0.2 4,423,332 - +0.9 4,423,371 - +0.5 4,423,411 - -0.7 4,423,466 - +0.5 4,423,466 - -1.3 4,423,488 - +0.3 4,423,488 - -1.9 4,423,488 - -0.9 4,423,488 - -0.5 4,423,552 - -0.4 4,423,552 - +1.6 4,423,570 - +0.3 4,423,570 - +0.4 4,423,570 - +0.8 4,423,570 - -0.0 4,423,570 - -0.7 4,423,570 - -0.9 4,423,681 - +0.8 4,423,681 - +1.5 4,423,681 - -1.2 4,423,691 - +0.3 4,423,842 + Pf1N1B4_3982 0.42 -1.9 4,423,843 - Pf1N1B4_3982 0.42 -0.1 4,423,843 - Pf1N1B4_3982 0.42 -0.3 4,423,942 + Pf1N1B4_3982 0.71 +0.3 4,423,942 + Pf1N1B4_3982 0.71 +0.5 4,423,943 - Pf1N1B4_3982 0.72 -0.2 4,423,943 - +1.3 4,423,945 + Pf1N1B4_3982 0.72 -0.0 4,423,945 + Pf1N1B4_3982 0.72 +0.3 4,423,945 + Pf1N1B4_3982 0.72 +0.1 4,423,946 - Pf1N1B4_3982 0.73 +0.3 4,423,946 - Pf1N1B4_3982 0.73 +0.2 4,424,060 + -0.7 4,424,060 + +0.9 4,424,061 - -0.2 4,424,085 + +2.3 4,424,085 + -0.3 4,424,086 - -0.0 4,424,088 + +0.3 4,424,088 + -1.5 4,424,088 + -1.7 4,424,089 - -2.3 4,424,089 - -1.7 4,424,089 - -0.3 4,424,089 - +0.3 4,424,092 + -0.3 4,424,092 + +0.5 4,424,092 + +0.3 4,424,092 + +0.4 4,424,092 + -2.9 4,424,092 + +0.3 4,424,092 + -0.7 4,424,092 + +0.5 4,424,093 - -0.5 4,424,093 - -0.7 4,424,093 - +0.6 4,424,098 - +1.0 4,424,121 + +0.3 4,424,121 + -0.5 4,424,156 + +0.5 4,424,231 - -1.7 4,424,231 - Pf1N1B4_3983 0.11 -1.1 4,424,282 + Pf1N1B4_3983 0.18 -1.4 4,424,282 + +1.3 4,424,344 - Pf1N1B4_3983 0.27 -1.3 4,424,344 - Pf1N1B4_3983 0.27 -1.3 4,424,415 + Pf1N1B4_3983 0.38 +1.9 4,424,415 + Pf1N1B4_3983 0.38 -0.9 4,424,416 - +1.3 4,424,482 + Pf1N1B4_3983 0.48 +0.9 4,424,538 + Pf1N1B4_3983 0.56 -0.4 4,424,807 - -2.0 4,424,833 - -1.3 4,424,935 + -0.7 4,424,936 - -0.0 4,424,936 - -0.9 4,425,130 + Pf1N1B4_3984 0.13 +1.1 4,425,131 - Pf1N1B4_3984 0.13 +0.1 4,425,131 - Pf1N1B4_3984 0.13 +0.6 4,425,169 + Pf1N1B4_3984 0.15 -1.5 4,425,170 - Pf1N1B4_3984 0.15 -0.0 4,425,170 - Pf1N1B4_3984 0.15 +0.8 4,425,170 - Pf1N1B4_3984 0.15 +0.8 4,425,236 - Pf1N1B4_3984 0.19 +1.9 4,425,671 - Pf1N1B4_3984 0.41 -1.3
Or see this region's nucleotide sequence