Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3793 and Pf1N1B4_3794 overlap by 2 nucleotides Pf1N1B4_3794 and Pf1N1B4_3795 are separated by 477 nucleotides Pf1N1B4_3795 and Pf1N1B4_3796 are separated by 24 nucleotides
Pf1N1B4_3793 - COG2010: Cytochrome c, mono- and diheme variants, at 4,201,328 to 4,202,149
_3793
Pf1N1B4_3794 - hypothetical protein, at 4,202,148 to 4,202,318
_3794
Pf1N1B4_3795 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,202,796 to 4,204,001
_3795
Pf1N1B4_3796 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 4,204,026 to 4,206,170
_3796
Position (kb) 4202
4203
4204
4205 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4201.843 kb on + strand, within Pf1N1B4_3793 at 4201.956 kb on + strand, within Pf1N1B4_3793 at 4201.957 kb on - strand, within Pf1N1B4_3793 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.216 kb on - strand, within Pf1N1B4_3794 at 4202.290 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.300 kb on + strand, within Pf1N1B4_3794 at 4202.301 kb on - strand at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.301 kb on - strand, within Pf1N1B4_3794 at 4202.416 kb on - strand at 4202.457 kb on - strand at 4202.464 kb on + strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.465 kb on - strand at 4202.492 kb on + strand at 4202.492 kb on + strand at 4202.514 kb on - strand at 4202.547 kb on - strand at 4202.576 kb on - strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.687 kb on + strand at 4202.700 kb on + strand at 4202.700 kb on + strand at 4202.701 kb on - strand at 4202.812 kb on + strand at 4203.513 kb on + strand, within Pf1N1B4_3795 at 4203.545 kb on + strand at 4203.545 kb on + strand, within Pf1N1B4_3795 at 4203.546 kb on - strand, within Pf1N1B4_3795 at 4204.123 kb on - strand at 4204.123 kb on - strand at 4204.276 kb on - strand, within Pf1N1B4_3796 at 4204.525 kb on - strand, within Pf1N1B4_3796 at 4204.731 kb on - strand, within Pf1N1B4_3796 at 4204.965 kb on + strand, within Pf1N1B4_3796
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,201,843 + Pf1N1B4_3793 0.63 +1.9 4,201,956 + Pf1N1B4_3793 0.76 +1.4 4,201,957 - Pf1N1B4_3793 0.77 +0.9 4,202,216 - Pf1N1B4_3794 0.40 +1.0 4,202,216 - Pf1N1B4_3794 0.40 +0.4 4,202,290 + Pf1N1B4_3794 0.83 +0.6 4,202,300 + Pf1N1B4_3794 0.89 +1.4 4,202,300 + Pf1N1B4_3794 0.89 -0.0 4,202,301 - -0.6 4,202,301 - Pf1N1B4_3794 0.89 -0.9 4,202,301 - Pf1N1B4_3794 0.89 -0.6 4,202,416 - +1.4 4,202,457 - +0.1 4,202,464 + +0.6 4,202,465 - +0.5 4,202,465 - -2.2 4,202,465 - -1.3 4,202,492 + +0.4 4,202,492 + +0.9 4,202,514 - +0.1 4,202,547 - +1.2 4,202,576 - -1.4 4,202,687 + -1.6 4,202,687 + +0.3 4,202,687 + -0.0 4,202,687 + +0.1 4,202,700 + +1.1 4,202,700 + +2.3 4,202,701 - -0.1 4,202,812 + +0.3 4,203,513 + Pf1N1B4_3795 0.59 +0.4 4,203,545 + -0.6 4,203,545 + Pf1N1B4_3795 0.62 -2.2 4,203,546 - Pf1N1B4_3795 0.62 -0.6 4,204,123 - -0.2 4,204,123 - -2.4 4,204,276 - Pf1N1B4_3796 0.12 +0.9 4,204,525 - Pf1N1B4_3796 0.23 -1.4 4,204,731 - Pf1N1B4_3796 0.33 -0.4 4,204,965 + Pf1N1B4_3796 0.44 +0.7
Or see this region's nucleotide sequence