Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_32

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_31 and Pf1N1B4_32 are separated by 179 nucleotidesPf1N1B4_32 and Pf1N1B4_33 are separated by 174 nucleotides Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017 _31 Pf1N1B4_32 - Transcriptional regulator, TetR family, at 37,197 to 37,817 _32 Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422 _33 Position (kb) 37 38Strain fitness (log2 ratio) -2 -1 0 1 2at 36.245 kb on + strandat 36.245 kb on + strand, within Pf1N1B4_31at 36.370 kb on + strand, within Pf1N1B4_31at 36.370 kb on + strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.479 kb on - strand, within Pf1N1B4_31at 36.560 kb on - strand, within Pf1N1B4_31at 36.750 kb on + strand, within Pf1N1B4_31at 36.751 kb on - strand, within Pf1N1B4_31at 36.751 kb on - strand, within Pf1N1B4_31at 37.017 kb on + strandat 37.267 kb on + strandat 37.465 kb on + strand, within Pf1N1B4_32at 37.482 kb on - strandat 37.558 kb on - strand, within Pf1N1B4_32at 37.608 kb on - strandat 37.657 kb on + strand, within Pf1N1B4_32at 37.657 kb on + strand, within Pf1N1B4_32at 37.657 kb on + strand, within Pf1N1B4_32at 37.658 kb on - strand, within Pf1N1B4_32at 37.658 kb on - strandat 37.658 kb on - strandat 37.658 kb on - strandat 37.658 kb on - strand, within Pf1N1B4_32at 37.658 kb on - strand, within Pf1N1B4_32at 37.658 kb on - strand, within Pf1N1B4_32at 37.658 kb on - strandat 37.687 kb on - strand, within Pf1N1B4_32at 37.933 kb on + strandat 37.934 kb on - strandat 38.084 kb on + strandat 38.141 kb on - strand, within Pf1N1B4_33at 38.295 kb on + strand, within Pf1N1B4_33at 38.316 kb on + strand, within Pf1N1B4_33at 38.317 kb on - strand, within Pf1N1B4_33at 38.317 kb on - strand, within Pf1N1B4_33at 38.463 kb on + strandat 38.463 kb on + strand, within Pf1N1B4_33at 38.464 kb on - strand, within Pf1N1B4_33at 38.515 kb on + strand, within Pf1N1B4_33at 38.515 kb on + strand, within Pf1N1B4_33at 38.515 kb on + strand, within Pf1N1B4_33at 38.515 kb on + strand, within Pf1N1B4_33at 38.516 kb on - strand, within Pf1N1B4_33at 38.516 kb on - strand, within Pf1N1B4_33at 38.703 kb on + strandat 38.703 kb on + strandat 38.703 kb on + strand, within Pf1N1B4_33at 38.703 kb on + strand, within Pf1N1B4_33at 38.704 kb on - strandat 38.704 kb on - strandat 38.704 kb on - strand, within Pf1N1B4_33at 38.706 kb on - strand, within Pf1N1B4_33at 38.721 kb on - strand, within Pf1N1B4_33

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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36,245 + +2.7
36,245 + Pf1N1B4_31 0.31 -0.1
36,370 + Pf1N1B4_31 0.42 +0.6
36,370 + Pf1N1B4_31 0.42 -0.4
36,479 - Pf1N1B4_31 0.52 -2.2
36,479 - Pf1N1B4_31 0.52 -2.0
36,479 - Pf1N1B4_31 0.52 -0.5
36,479 - Pf1N1B4_31 0.52 -0.4
36,560 - Pf1N1B4_31 0.59 +0.0
36,750 + Pf1N1B4_31 0.76 -0.6
36,751 - Pf1N1B4_31 0.76 -0.3
36,751 - Pf1N1B4_31 0.76 +1.0
37,017 + +0.4
37,267 + +0.4
37,465 + Pf1N1B4_32 0.43 -0.2
37,482 - -1.5
37,558 - Pf1N1B4_32 0.58 -2.0
37,608 - -0.1
37,657 + Pf1N1B4_32 0.74 +0.9
37,657 + Pf1N1B4_32 0.74 -1.2
37,657 + Pf1N1B4_32 0.74 +0.4
37,658 - Pf1N1B4_32 0.74 -0.1
37,658 - -0.2
37,658 - +0.4
37,658 - -0.2
37,658 - Pf1N1B4_32 0.74 -1.5
37,658 - Pf1N1B4_32 0.74 -1.0
37,658 - Pf1N1B4_32 0.74 +0.4
37,658 - -1.2
37,687 - Pf1N1B4_32 0.79 -1.6
37,933 + -1.8
37,934 - +1.9
38,084 + -0.1
38,141 - Pf1N1B4_33 0.10 -0.6
38,295 + Pf1N1B4_33 0.21 -0.3
38,316 + Pf1N1B4_33 0.23 +0.0
38,317 - Pf1N1B4_33 0.23 -0.8
38,317 - Pf1N1B4_33 0.23 +0.9
38,463 + +0.4
38,463 + Pf1N1B4_33 0.33 +2.2
38,464 - Pf1N1B4_33 0.33 -0.1
38,515 + Pf1N1B4_33 0.37 -0.8
38,515 + Pf1N1B4_33 0.37 -0.3
38,515 + Pf1N1B4_33 0.37 +0.0
38,515 + Pf1N1B4_33 0.37 +2.7
38,516 - Pf1N1B4_33 0.37 -0.3
38,516 - Pf1N1B4_33 0.37 -0.6
38,703 + -0.6
38,703 + +0.4
38,703 + Pf1N1B4_33 0.50 -0.3
38,703 + Pf1N1B4_33 0.50 +0.6
38,704 - +0.4
38,704 - +0.4
38,704 - Pf1N1B4_33 0.50 +0.1
38,706 - Pf1N1B4_33 0.50 -0.2
38,721 - Pf1N1B4_33 0.51 +0.7

Or see this region's nucleotide sequence