Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3009 and Pf1N1B4_3010 are separated by 118 nucleotides Pf1N1B4_3010 and Pf1N1B4_3011 are separated by 133 nucleotides
Pf1N1B4_3009 - Protein-export membrane protein SecF (TC 3.A.5.1.1), at 3,383,617 to 3,384,531
_3009
Pf1N1B4_3010 - probable exported protein YPO1624, at 3,384,650 to 3,385,201
_3010
Pf1N1B4_3011 - Tyrosine recombinase XerD, at 3,385,335 to 3,386,231
_3011
Position (kb)
3384
3385
3386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3384.594 kb on - strand at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.722 kb on + strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.723 kb on - strand, within Pf1N1B4_3010 at 3384.734 kb on - strand at 3384.750 kb on - strand, within Pf1N1B4_3010 at 3384.844 kb on - strand, within Pf1N1B4_3010 at 3384.923 kb on - strand, within Pf1N1B4_3010 at 3385.009 kb on + strand, within Pf1N1B4_3010 at 3385.009 kb on + strand, within Pf1N1B4_3010 at 3385.009 kb on + strand, within Pf1N1B4_3010 at 3385.010 kb on - strand, within Pf1N1B4_3010 at 3385.013 kb on + strand at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.013 kb on + strand, within Pf1N1B4_3010 at 3385.014 kb on - strand, within Pf1N1B4_3010 at 3385.014 kb on - strand, within Pf1N1B4_3010 at 3385.014 kb on - strand, within Pf1N1B4_3010 at 3385.014 kb on - strand at 3385.014 kb on - strand, within Pf1N1B4_3010 at 3385.036 kb on + strand, within Pf1N1B4_3010 at 3385.037 kb on - strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.068 kb on + strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.069 kb on - strand, within Pf1N1B4_3010 at 3385.074 kb on - strand, within Pf1N1B4_3010 at 3385.199 kb on + strand at 3385.199 kb on + strand at 3385.199 kb on + strand at 3385.199 kb on + strand at 3385.200 kb on - strand at 3385.261 kb on + strand at 3385.262 kb on - strand at 3385.262 kb on - strand at 3385.262 kb on - strand at 3385.263 kb on + strand at 3385.263 kb on + strand at 3385.263 kb on + strand at 3385.264 kb on - strand at 3385.264 kb on - strand at 3385.264 kb on - strand at 3385.264 kb on - strand at 3385.283 kb on + strand at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.536 kb on + strand, within Pf1N1B4_3011 at 3385.585 kb on - strand, within Pf1N1B4_3011 at 3385.591 kb on - strand, within Pf1N1B4_3011 at 3385.667 kb on + strand, within Pf1N1B4_3011 at 3385.668 kb on - strand, within Pf1N1B4_3011 at 3385.702 kb on + strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3385.912 kb on - strand, within Pf1N1B4_3011 at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.169 kb on + strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand at 3386.170 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,384,594 - +0.5 3,384,722 + Pf1N1B4_3010 0.13 +0.4 3,384,722 + Pf1N1B4_3010 0.13 -2.8 3,384,722 + Pf1N1B4_3010 0.13 +0.1 3,384,722 + Pf1N1B4_3010 0.13 -0.6 3,384,722 + Pf1N1B4_3010 0.13 +0.5 3,384,722 + Pf1N1B4_3010 0.13 -0.0 3,384,723 - Pf1N1B4_3010 0.13 -1.3 3,384,723 - Pf1N1B4_3010 0.13 +0.0 3,384,723 - Pf1N1B4_3010 0.13 -2.5 3,384,723 - Pf1N1B4_3010 0.13 +0.1 3,384,723 - Pf1N1B4_3010 0.13 +0.2 3,384,723 - Pf1N1B4_3010 0.13 -1.9 3,384,723 - Pf1N1B4_3010 0.13 -1.1 3,384,723 - Pf1N1B4_3010 0.13 +0.1 3,384,734 - +1.3 3,384,750 - Pf1N1B4_3010 0.18 +1.6 3,384,844 - Pf1N1B4_3010 0.35 -0.8 3,384,923 - Pf1N1B4_3010 0.49 -0.5 3,385,009 + Pf1N1B4_3010 0.65 -1.6 3,385,009 + Pf1N1B4_3010 0.65 -0.3 3,385,009 + Pf1N1B4_3010 0.65 -0.2 3,385,010 - Pf1N1B4_3010 0.65 -0.2 3,385,013 + -1.9 3,385,013 + Pf1N1B4_3010 0.66 -0.7 3,385,013 + Pf1N1B4_3010 0.66 -2.6 3,385,013 + Pf1N1B4_3010 0.66 -0.1 3,385,013 + Pf1N1B4_3010 0.66 +0.7 3,385,013 + Pf1N1B4_3010 0.66 -0.2 3,385,013 + Pf1N1B4_3010 0.66 +0.0 3,385,013 + Pf1N1B4_3010 0.66 +0.3 3,385,013 + Pf1N1B4_3010 0.66 -0.1 3,385,014 - Pf1N1B4_3010 0.66 +1.9 3,385,014 - Pf1N1B4_3010 0.66 +0.7 3,385,014 - Pf1N1B4_3010 0.66 +0.2 3,385,014 - -0.3 3,385,014 - Pf1N1B4_3010 0.66 +0.8 3,385,036 + Pf1N1B4_3010 0.70 +1.0 3,385,037 - Pf1N1B4_3010 0.70 -0.9 3,385,068 + Pf1N1B4_3010 0.76 -0.3 3,385,068 + Pf1N1B4_3010 0.76 +0.1 3,385,068 + Pf1N1B4_3010 0.76 -1.3 3,385,068 + Pf1N1B4_3010 0.76 +0.7 3,385,068 + Pf1N1B4_3010 0.76 -1.9 3,385,068 + Pf1N1B4_3010 0.76 +0.4 3,385,068 + Pf1N1B4_3010 0.76 -0.1 3,385,069 - Pf1N1B4_3010 0.76 -0.6 3,385,069 - Pf1N1B4_3010 0.76 -1.1 3,385,069 - Pf1N1B4_3010 0.76 -0.4 3,385,069 - Pf1N1B4_3010 0.76 -0.0 3,385,069 - Pf1N1B4_3010 0.76 -0.3 3,385,069 - Pf1N1B4_3010 0.76 -0.2 3,385,069 - Pf1N1B4_3010 0.76 -0.3 3,385,069 - Pf1N1B4_3010 0.76 -0.3 3,385,074 - Pf1N1B4_3010 0.77 -0.3 3,385,199 + +2.3 3,385,199 + -0.3 3,385,199 + +0.0 3,385,199 + +0.9 3,385,200 - -1.3 3,385,261 + -1.1 3,385,262 - -0.6 3,385,262 - -0.4 3,385,262 - +0.4 3,385,263 + -1.0 3,385,263 + -0.5 3,385,263 + +0.1 3,385,264 - +0.1 3,385,264 - -0.9 3,385,264 - -0.3 3,385,264 - -0.2 3,385,283 + -0.3 3,385,536 + Pf1N1B4_3011 0.22 +0.7 3,385,536 + Pf1N1B4_3011 0.22 -1.5 3,385,585 - Pf1N1B4_3011 0.28 -0.9 3,385,591 - Pf1N1B4_3011 0.29 -0.9 3,385,667 + Pf1N1B4_3011 0.37 +1.3 3,385,668 - Pf1N1B4_3011 0.37 -1.9 3,385,702 + Pf1N1B4_3011 0.41 +0.7 3,385,912 - Pf1N1B4_3011 0.64 -2.1 3,385,912 - Pf1N1B4_3011 0.64 -0.3 3,386,169 + -0.4 3,386,169 + -1.1 3,386,169 + +1.0 3,386,169 + +1.7 3,386,169 + -1.6 3,386,170 - -0.9 3,386,170 - -0.6 3,386,170 - -0.6 3,386,170 - -2.5
Or see this region's nucleotide sequence