Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2990 and Pf1N1B4_2991 are separated by 42 nucleotides Pf1N1B4_2991 and Pf1N1B4_2992 are separated by 100 nucleotides Pf1N1B4_2992 and Pf1N1B4_2993 are separated by 154 nucleotides Pf1N1B4_2993 and Pf1N1B4_2994 are separated by 406 nucleotides
Pf1N1B4_2990 - NAD-dependent formate dehydrogenase gamma subunit, at 3,359,418 to 3,359,894
_2990
Pf1N1B4_2991 - hypothetical protein, at 3,359,937 to 3,360,206
_2991
Pf1N1B4_2992 - Potassium channel protein, at 3,360,307 to 3,360,648
_2992
Pf1N1B4_2993 - hypothetical protein, at 3,360,803 to 3,361,132
_2993
Pf1N1B4_2994 - ABC transporter, ATP-binding protein-related protein, at 3,361,539 to 3,363,155
_2994
Position (kb)
3360
3361
3362 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3359.868 kb on - strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.894 kb on + strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3359.895 kb on - strand at 3360.068 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.145 kb on + strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.146 kb on - strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.151 kb on + strand, within Pf1N1B4_2991 at 3360.152 kb on - strand, within Pf1N1B4_2991 at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.186 kb on + strand at 3360.197 kb on + strand at 3360.198 kb on - strand at 3360.252 kb on - strand at 3360.287 kb on - strand at 3360.346 kb on + strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.347 kb on - strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.364 kb on + strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.365 kb on - strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.469 kb on + strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.470 kb on - strand, within Pf1N1B4_2992 at 3360.584 kb on + strand, within Pf1N1B4_2992 at 3360.585 kb on - strand, within Pf1N1B4_2992 at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.646 kb on + strand at 3360.722 kb on + strand at 3360.722 kb on + strand at 3360.787 kb on - strand at 3360.829 kb on + strand at 3360.830 kb on - strand at 3360.830 kb on - strand at 3360.919 kb on + strand, within Pf1N1B4_2993 at 3360.920 kb on - strand, within Pf1N1B4_2993 at 3360.920 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.923 kb on - strand, within Pf1N1B4_2993 at 3360.970 kb on + strand, within Pf1N1B4_2993 at 3361.030 kb on - strand, within Pf1N1B4_2993 at 3361.030 kb on - strand, within Pf1N1B4_2993 at 3361.083 kb on + strand, within Pf1N1B4_2993 at 3361.083 kb on + strand, within Pf1N1B4_2993 at 3361.084 kb on - strand at 3361.084 kb on - strand, within Pf1N1B4_2993 at 3361.250 kb on - strand at 3361.370 kb on - strand at 3361.370 kb on - strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.375 kb on + strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.376 kb on - strand at 3361.395 kb on + strand at 3361.395 kb on + strand at 3361.396 kb on - strand at 3361.396 kb on - strand at 3361.437 kb on - strand at 3361.452 kb on + strand at 3361.453 kb on - strand at 3361.453 kb on - strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.472 kb on + strand at 3361.473 kb on - strand at 3361.473 kb on - strand at 3361.477 kb on - strand at 3361.477 kb on - strand at 3361.497 kb on + strand at 3361.497 kb on + strand at 3361.498 kb on - strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.503 kb on + strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.504 kb on - strand at 3361.522 kb on + strand at 3361.523 kb on - strand at 3361.523 kb on - strand at 3361.536 kb on - strand at 3361.536 kb on - strand at 3361.536 kb on - strand at 3361.587 kb on - strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.643 kb on + strand at 3361.644 kb on - strand at 3361.644 kb on - strand at 3361.656 kb on + strand at 3361.684 kb on + strand at 3361.684 kb on + strand at 3361.684 kb on + strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.685 kb on - strand at 3361.730 kb on - strand at 3361.739 kb on - strand at 3361.810 kb on + strand, within Pf1N1B4_2994 at 3361.846 kb on + strand, within Pf1N1B4_2994 at 3361.847 kb on - strand, within Pf1N1B4_2994 at 3361.876 kb on + strand, within Pf1N1B4_2994 at 3361.877 kb on - strand, within Pf1N1B4_2994 at 3361.877 kb on - strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.879 kb on + strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.880 kb on - strand, within Pf1N1B4_2994 at 3361.949 kb on + strand, within Pf1N1B4_2994 at 3361.955 kb on + strand, within Pf1N1B4_2994 at 3361.956 kb on - strand, within Pf1N1B4_2994 at 3361.997 kb on - strand, within Pf1N1B4_2994 at 3362.014 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.062 kb on + strand, within Pf1N1B4_2994 at 3362.063 kb on - strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.064 kb on + strand, within Pf1N1B4_2994 at 3362.065 kb on - strand, within Pf1N1B4_2994 at 3362.066 kb on + strand at 3362.066 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,359,868 - +1.1 3,359,894 + +0.2 3,359,894 + +2.3 3,359,894 + -0.9 3,359,895 - -0.5 3,359,895 - +0.1 3,359,895 - +0.1 3,360,068 + Pf1N1B4_2991 0.49 +0.1 3,360,145 + Pf1N1B4_2991 0.77 -0.5 3,360,145 + Pf1N1B4_2991 0.77 +0.4 3,360,146 - Pf1N1B4_2991 0.77 -1.1 3,360,146 - Pf1N1B4_2991 0.77 -0.9 3,360,146 - Pf1N1B4_2991 0.77 -1.3 3,360,146 - Pf1N1B4_2991 0.77 +1.5 3,360,146 - Pf1N1B4_2991 0.77 -0.9 3,360,151 + Pf1N1B4_2991 0.79 +1.2 3,360,151 + Pf1N1B4_2991 0.79 -1.3 3,360,151 + Pf1N1B4_2991 0.79 +1.0 3,360,151 + Pf1N1B4_2991 0.79 -0.2 3,360,152 - Pf1N1B4_2991 0.80 +0.0 3,360,186 + -0.6 3,360,186 + -1.2 3,360,186 + +0.7 3,360,186 + +0.7 3,360,197 + -0.9 3,360,198 - -0.1 3,360,252 - +1.3 3,360,287 - +0.3 3,360,346 + Pf1N1B4_2992 0.11 -0.7 3,360,347 - Pf1N1B4_2992 0.12 -2.7 3,360,347 - Pf1N1B4_2992 0.12 -0.8 3,360,347 - -1.9 3,360,347 - Pf1N1B4_2992 0.12 -2.5 3,360,347 - Pf1N1B4_2992 0.12 -2.7 3,360,364 + Pf1N1B4_2992 0.17 -0.3 3,360,364 + Pf1N1B4_2992 0.17 +0.7 3,360,365 - Pf1N1B4_2992 0.17 +0.2 3,360,365 - Pf1N1B4_2992 0.17 +0.1 3,360,365 - Pf1N1B4_2992 0.17 -1.3 3,360,365 - Pf1N1B4_2992 0.17 -0.9 3,360,365 - Pf1N1B4_2992 0.17 +0.9 3,360,469 + Pf1N1B4_2992 0.47 -1.6 3,360,469 + Pf1N1B4_2992 0.47 -0.7 3,360,469 + Pf1N1B4_2992 0.47 -1.0 3,360,469 + Pf1N1B4_2992 0.47 -0.9 3,360,469 + Pf1N1B4_2992 0.47 +1.0 3,360,469 + Pf1N1B4_2992 0.47 +1.3 3,360,469 + Pf1N1B4_2992 0.47 -0.6 3,360,469 + Pf1N1B4_2992 0.47 +0.1 3,360,470 - Pf1N1B4_2992 0.48 +0.3 3,360,470 - Pf1N1B4_2992 0.48 -0.5 3,360,470 - Pf1N1B4_2992 0.48 -0.4 3,360,470 - Pf1N1B4_2992 0.48 -1.6 3,360,470 - Pf1N1B4_2992 0.48 -0.9 3,360,470 - Pf1N1B4_2992 0.48 +0.4 3,360,470 - Pf1N1B4_2992 0.48 +1.4 3,360,470 - Pf1N1B4_2992 0.48 -0.0 3,360,584 + Pf1N1B4_2992 0.81 +0.5 3,360,585 - Pf1N1B4_2992 0.81 +1.6 3,360,646 + +2.3 3,360,646 + -0.3 3,360,646 + -0.3 3,360,646 + +1.4 3,360,722 + +0.0 3,360,722 + -0.3 3,360,787 - -0.5 3,360,829 + -0.4 3,360,830 - -0.7 3,360,830 - +0.4 3,360,919 + Pf1N1B4_2993 0.35 +0.4 3,360,920 - Pf1N1B4_2993 0.35 -0.3 3,360,920 - Pf1N1B4_2993 0.35 -0.1 3,360,923 - Pf1N1B4_2993 0.36 -0.4 3,360,923 - Pf1N1B4_2993 0.36 -0.9 3,360,923 - Pf1N1B4_2993 0.36 +0.3 3,360,923 - Pf1N1B4_2993 0.36 -1.1 3,360,923 - Pf1N1B4_2993 0.36 -1.4 3,360,923 - Pf1N1B4_2993 0.36 -1.1 3,360,970 + Pf1N1B4_2993 0.51 -0.3 3,361,030 - Pf1N1B4_2993 0.69 +0.3 3,361,030 - Pf1N1B4_2993 0.69 -1.9 3,361,083 + Pf1N1B4_2993 0.85 -0.0 3,361,083 + Pf1N1B4_2993 0.85 -0.1 3,361,084 - +0.1 3,361,084 - Pf1N1B4_2993 0.85 -0.7 3,361,250 - +0.9 3,361,370 - +1.5 3,361,370 - -0.8 3,361,375 + +1.3 3,361,375 + -0.2 3,361,375 + +0.3 3,361,375 + -1.7 3,361,376 - -0.6 3,361,376 - -1.1 3,361,376 - -0.9 3,361,376 - -0.6 3,361,376 - -0.0 3,361,395 + -0.3 3,361,395 + +0.2 3,361,396 - -2.9 3,361,396 - +0.7 3,361,437 - -0.1 3,361,452 + +1.0 3,361,453 - -0.0 3,361,453 - +0.3 3,361,472 + -2.3 3,361,472 + -1.5 3,361,472 + -0.5 3,361,472 + +1.6 3,361,473 - +0.7 3,361,473 - +1.1 3,361,477 - -0.3 3,361,477 - -0.6 3,361,497 + -0.9 3,361,497 + -0.3 3,361,498 - +0.7 3,361,503 + +1.2 3,361,503 + -0.4 3,361,503 + -1.2 3,361,503 + -2.3 3,361,503 + -1.3 3,361,504 - +1.1 3,361,504 - -3.1 3,361,504 - +0.0 3,361,504 - +0.5 3,361,504 - -0.5 3,361,522 + -0.3 3,361,523 - +0.4 3,361,523 - +0.1 3,361,536 - +0.2 3,361,536 - +1.0 3,361,536 - +0.9 3,361,587 - +0.1 3,361,643 + +0.4 3,361,643 + -0.1 3,361,643 + -1.6 3,361,643 + -0.9 3,361,644 - -0.6 3,361,644 - -1.9 3,361,656 + +1.7 3,361,684 + +0.5 3,361,684 + +0.7 3,361,684 + +0.7 3,361,685 - -0.3 3,361,685 - +1.2 3,361,685 - -1.3 3,361,685 - -0.1 3,361,730 - +2.3 3,361,739 - -0.0 3,361,810 + Pf1N1B4_2994 0.17 +1.3 3,361,846 + Pf1N1B4_2994 0.19 -0.3 3,361,847 - Pf1N1B4_2994 0.19 -0.9 3,361,876 + Pf1N1B4_2994 0.21 -2.3 3,361,877 - Pf1N1B4_2994 0.21 +1.1 3,361,877 - Pf1N1B4_2994 0.21 +1.0 3,361,879 + Pf1N1B4_2994 0.21 +0.1 3,361,879 + Pf1N1B4_2994 0.21 +0.7 3,361,879 + Pf1N1B4_2994 0.21 +1.2 3,361,880 - Pf1N1B4_2994 0.21 -2.1 3,361,880 - Pf1N1B4_2994 0.21 +0.3 3,361,880 - Pf1N1B4_2994 0.21 +0.0 3,361,949 + Pf1N1B4_2994 0.25 -0.9 3,361,955 + Pf1N1B4_2994 0.26 -0.9 3,361,956 - Pf1N1B4_2994 0.26 +0.7 3,361,997 - Pf1N1B4_2994 0.28 +0.1 3,362,014 + Pf1N1B4_2994 0.29 -0.7 3,362,062 + Pf1N1B4_2994 0.32 +0.2 3,362,062 + Pf1N1B4_2994 0.32 +2.7 3,362,062 + Pf1N1B4_2994 0.32 -1.0 3,362,062 + Pf1N1B4_2994 0.32 +0.4 3,362,063 - Pf1N1B4_2994 0.32 +0.2 3,362,064 + Pf1N1B4_2994 0.32 -2.1 3,362,064 + Pf1N1B4_2994 0.32 +1.4 3,362,064 + Pf1N1B4_2994 0.32 -0.3 3,362,064 + Pf1N1B4_2994 0.32 +0.3 3,362,065 - Pf1N1B4_2994 0.33 -1.3 3,362,066 + +0.7 3,362,066 + +0.7
Or see this region's nucleotide sequence