Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_224

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_223 and Pf1N1B4_224 overlap by 4 nucleotidesPf1N1B4_224 and Pf1N1B4_225 are separated by 116 nucleotides Pf1N1B4_223 - Citronellyl-CoA dehydrogenase, at 283,755 to 284,912 _223 Pf1N1B4_224 - Isohexenylglutaconyl-CoA hydratase, at 284,909 to 285,706 _224 Pf1N1B4_225 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 285,823 to 287,784 _225 Position (kb) 284 285 286Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 283.948 kb on - strand, within Pf1N1B4_223at 283.948 kb on - strand, within Pf1N1B4_223at 283.948 kb on - strand, within Pf1N1B4_223at 283.954 kb on - strand, within Pf1N1B4_223at 284.156 kb on - strand, within Pf1N1B4_223at 284.196 kb on + strand, within Pf1N1B4_223at 284.196 kb on + strand, within Pf1N1B4_223at 284.197 kb on - strandat 284.197 kb on - strand, within Pf1N1B4_223at 284.197 kb on - strand, within Pf1N1B4_223at 284.332 kb on + strand, within Pf1N1B4_223at 284.333 kb on - strand, within Pf1N1B4_223at 284.520 kb on + strand, within Pf1N1B4_223at 284.520 kb on + strand, within Pf1N1B4_223at 284.520 kb on + strand, within Pf1N1B4_223at 284.520 kb on + strand, within Pf1N1B4_223at 284.521 kb on - strand, within Pf1N1B4_223at 284.544 kb on + strand, within Pf1N1B4_223at 284.653 kb on - strand, within Pf1N1B4_223at 284.670 kb on + strandat 284.670 kb on + strand, within Pf1N1B4_223at 284.670 kb on + strand, within Pf1N1B4_223at 284.671 kb on - strand, within Pf1N1B4_223at 284.671 kb on - strand, within Pf1N1B4_223at 285.288 kb on + strand, within Pf1N1B4_224at 285.289 kb on - strand, within Pf1N1B4_224at 285.289 kb on - strand, within Pf1N1B4_224at 285.289 kb on - strand, within Pf1N1B4_224at 285.806 kb on + strandat 285.806 kb on + strandat 285.806 kb on + strandat 285.807 kb on - strandat 285.807 kb on - strandat 285.807 kb on - strandat 285.807 kb on - strandat 285.807 kb on - strandat 285.807 kb on - strandat 285.920 kb on + strandat 286.006 kb on + strandat 286.007 kb on - strandat 286.007 kb on - strandat 286.495 kb on + strand, within Pf1N1B4_225at 286.495 kb on + strand, within Pf1N1B4_225at 286.631 kb on - strand, within Pf1N1B4_225at 286.631 kb on - strand, within Pf1N1B4_225at 286.631 kb on - strand, within Pf1N1B4_225at 286.631 kb on - strand, within Pf1N1B4_225at 286.682 kb on - strand, within Pf1N1B4_225at 286.682 kb on - strand, within Pf1N1B4_225

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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283,948 - Pf1N1B4_223 0.17 -1.1
283,948 - Pf1N1B4_223 0.17 +0.1
283,948 - Pf1N1B4_223 0.17 -0.6
283,954 - Pf1N1B4_223 0.17 -0.8
284,156 - Pf1N1B4_223 0.35 +0.0
284,196 + Pf1N1B4_223 0.38 -0.2
284,196 + Pf1N1B4_223 0.38 +0.4
284,197 - +0.7
284,197 - Pf1N1B4_223 0.38 +0.5
284,197 - Pf1N1B4_223 0.38 -0.2
284,332 + Pf1N1B4_223 0.50 +0.4
284,333 - Pf1N1B4_223 0.50 +0.8
284,520 + Pf1N1B4_223 0.66 +1.8
284,520 + Pf1N1B4_223 0.66 +0.6
284,520 + Pf1N1B4_223 0.66 +1.1
284,520 + Pf1N1B4_223 0.66 -0.6
284,521 - Pf1N1B4_223 0.66 -0.3
284,544 + Pf1N1B4_223 0.68 +0.8
284,653 - Pf1N1B4_223 0.78 -0.6
284,670 + -1.0
284,670 + Pf1N1B4_223 0.79 -1.1
284,670 + Pf1N1B4_223 0.79 +0.6
284,671 - Pf1N1B4_223 0.79 +0.4
284,671 - Pf1N1B4_223 0.79 -0.2
285,288 + Pf1N1B4_224 0.47 -2.8
285,289 - Pf1N1B4_224 0.48 -0.4
285,289 - Pf1N1B4_224 0.48 -1.4
285,289 - Pf1N1B4_224 0.48 -0.6
285,806 + -0.4
285,806 + -0.9
285,806 + -0.5
285,807 - +0.8
285,807 - -0.6
285,807 - +0.9
285,807 - -0.8
285,807 - -0.6
285,807 - -0.1
285,920 + +0.0
286,006 + +1.0
286,007 - -0.6
286,007 - -2.0
286,495 + Pf1N1B4_225 0.34 +0.8
286,495 + Pf1N1B4_225 0.34 +1.3
286,631 - Pf1N1B4_225 0.41 -0.4
286,631 - Pf1N1B4_225 0.41 -0.2
286,631 - Pf1N1B4_225 0.41 +0.4
286,631 - Pf1N1B4_225 0.41 -2.0
286,682 - Pf1N1B4_225 0.44 +1.9
286,682 - Pf1N1B4_225 0.44 -0.2

Or see this region's nucleotide sequence