Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMc04018 and SMc04019 are separated by 119 nucleotides SMc04019 and SMc04020 are separated by 103 nucleotides
SMc04018: SMc04018 - 5'-nucleotidase, at 3,014,867 to 3,016,753
SMc04018
SMc04019: SMc04019 - ferrochelatase, at 3,016,873 to 3,017,901
SMc04019
SMc04020: SMc04020 - hypothetical protein, at 3,018,005 to 3,018,991
SMc04020
Position (kb)
3016
3017
3018 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3016.199 kb on + strand, within SMc04018 at 3016.200 kb on - strand, within SMc04018 at 3016.295 kb on + strand, within SMc04018 at 3016.373 kb on + strand, within SMc04018 at 3016.373 kb on + strand, within SMc04018 at 3016.380 kb on - strand, within SMc04018 at 3016.413 kb on - strand, within SMc04018 at 3016.413 kb on - strand, within SMc04018 at 3016.442 kb on + strand, within SMc04018 at 3016.652 kb on + strand at 3016.652 kb on + strand at 3017.932 kb on + strand at 3017.932 kb on + strand at 3017.932 kb on + strand at 3017.932 kb on + strand at 3017.932 kb on + strand at 3017.933 kb on - strand at 3018.018 kb on - strand at 3018.099 kb on - strand at 3018.100 kb on + strand at 3018.100 kb on + strand at 3018.100 kb on + strand at 3018.101 kb on - strand at 3018.123 kb on - strand, within SMc04020 at 3018.285 kb on + strand, within SMc04020 at 3018.442 kb on - strand, within SMc04020 at 3018.771 kb on - strand, within SMc04020 at 3018.789 kb on - strand, within SMc04020 at 3018.886 kb on - strand, within SMc04020
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 3,016,199 + SMc04018 0.71 -2.1 3,016,200 - SMc04018 0.71 -0.6 3,016,295 + SMc04018 0.76 +0.3 3,016,373 + SMc04018 0.80 -1.2 3,016,373 + SMc04018 0.80 -1.0 3,016,380 - SMc04018 0.80 -0.3 3,016,413 - SMc04018 0.82 +0.4 3,016,413 - SMc04018 0.82 +1.2 3,016,442 + SMc04018 0.83 +1.2 3,016,652 + -0.1 3,016,652 + -0.3 3,017,932 + -0.2 3,017,932 + +0.6 3,017,932 + -0.1 3,017,932 + -1.5 3,017,932 + +0.4 3,017,933 - +0.8 3,018,018 - +0.2 3,018,099 - -0.2 3,018,100 + +0.4 3,018,100 + +0.9 3,018,100 + -0.5 3,018,101 - -0.2 3,018,123 - SMc04020 0.12 -1.0 3,018,285 + SMc04020 0.28 +2.3 3,018,442 - SMc04020 0.44 -2.7 3,018,771 - SMc04020 0.78 -0.1 3,018,789 - SMc04020 0.79 -2.5 3,018,886 - SMc04020 0.89 +4.0
Or see this region's nucleotide sequence