Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SMa0082 and SMa0083 are separated by 15 nucleotides SMa0083 and SMa0085 are separated by 17 nucleotides SMa0085 and SMa0087 are separated by 16 nucleotides SMa0087 and SMa0089 are separated by 282 nucleotides
SMa0082: SMa0082 - ABC transporter substrate-binding protein, at 43,471 to 44,286
SMa0082
SMa0083: SMa0083 - ABC transporter ATP-binding protein, at 44,302 to 45,081
SMa0083
SMa0085: SMa0085 - dehydrogenase, at 45,099 to 46,052
SMa0085
SMa0087: SMa0087 - hypothetical protein, at 46,069 to 46,401
SMa0087
SMa0089: SMa0089 - hypothetical protein, at 46,684 to 47,100
SMa0089
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 44.285 kb on - strand at 44.378 kb on + strand at 44.379 kb on - strand at 44.379 kb on - strand at 44.379 kb on - strand at 44.389 kb on + strand, within SMa0083 at 44.389 kb on + strand, within SMa0083 at 44.389 kb on + strand, within SMa0083 at 44.389 kb on + strand, within SMa0083 at 44.389 kb on + strand, within SMa0083 at 44.390 kb on - strand, within SMa0083 at 45.097 kb on - strand at 45.097 kb on - strand at 45.097 kb on - strand at 45.127 kb on + strand at 45.127 kb on + strand at 45.127 kb on + strand at 45.128 kb on - strand at 45.128 kb on - strand at 45.137 kb on - strand at 45.137 kb on - strand at 45.199 kb on - strand, within SMa0085 at 45.518 kb on - strand, within SMa0085 at 45.556 kb on + strand, within SMa0085 at 45.786 kb on - strand, within SMa0085 at 45.794 kb on - strand, within SMa0085 at 45.929 kb on + strand, within SMa0085 at 46.259 kb on - strand, within SMa0087 at 46.259 kb on - strand, within SMa0087 at 46.259 kb on - strand, within SMa0087 at 46.451 kb on - strand at 46.567 kb on + strand at 46.572 kb on - strand at 46.576 kb on + strand at 46.880 kb on + strand, within SMa0089 at 46.881 kb on - strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.920 kb on + strand, within SMa0089 at 46.921 kb on - strand, within SMa0089 at 46.922 kb on + strand, within SMa0089 at 46.923 kb on - strand, within SMa0089 at 46.923 kb on - strand, within SMa0089
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 44,285 - +0.1 44,378 + +1.4 44,379 - +1.4 44,379 - -0.6 44,379 - -1.3 44,389 + SMa0083 0.11 +0.4 44,389 + SMa0083 0.11 +1.5 44,389 + SMa0083 0.11 +0.8 44,389 + SMa0083 0.11 +0.8 44,389 + SMa0083 0.11 +0.3 44,390 - SMa0083 0.11 -0.1 45,097 - -0.3 45,097 - -0.1 45,097 - +0.0 45,127 + -0.4 45,127 + -2.3 45,127 + -1.4 45,128 - -0.3 45,128 - -0.2 45,137 - -2.7 45,137 - -1.2 45,199 - SMa0085 0.10 -1.7 45,518 - SMa0085 0.44 -3.2 45,556 + SMa0085 0.48 -0.8 45,786 - SMa0085 0.72 -1.0 45,794 - SMa0085 0.73 -1.9 45,929 + SMa0085 0.87 -0.5 46,259 - SMa0087 0.57 -0.7 46,259 - SMa0087 0.57 -0.7 46,259 - SMa0087 0.57 -1.3 46,451 - -0.2 46,567 + +0.2 46,572 - -1.6 46,576 + +1.1 46,880 + SMa0089 0.47 +1.5 46,881 - SMa0089 0.47 -1.1 46,920 + SMa0089 0.57 -0.4 46,920 + SMa0089 0.57 -0.4 46,920 + SMa0089 0.57 +0.2 46,921 - SMa0089 0.57 -2.0 46,922 + SMa0089 0.57 +0.8 46,923 - SMa0089 0.57 +0.4 46,923 - SMa0089 0.57 -0.5
Or see this region's nucleotide sequence