Experiment: NAG (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SM_b20615 and SM_b20616 are separated by 1 nucleotides SM_b20616 and SM_b20617 are separated by 196 nucleotides SM_b20617 and SM_b20618 overlap by 4 nucleotides
SM_b20615: SM_b20615 - thiamine biosynthesis protein ThiC, at 1,633,944 to 1,635,779
_b20615
SM_b20616: SM_b20616 - thiamine biosynthesis oxidoreductase, at 1,635,781 to 1,636,758
_b20616
SM_b20617: SM_b20617 - thiazole synthase, at 1,636,955 to 1,637,728
_b20617
SM_b20618: SM_b20618 - thiamine-phosphate pyrophosphorylase, at 1,637,725 to 1,638,330
_b20618
Position (kb)
1635
1636
1637 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1634.896 kb on - strand, within SM_b20615 at 1634.897 kb on + strand, within SM_b20615 at 1634.898 kb on - strand, within SM_b20615 at 1634.994 kb on + strand, within SM_b20615 at 1634.995 kb on - strand, within SM_b20615 at 1635.012 kb on - strand, within SM_b20615 at 1635.033 kb on + strand, within SM_b20615 at 1635.033 kb on + strand, within SM_b20615 at 1635.033 kb on + strand, within SM_b20615 at 1635.194 kb on + strand, within SM_b20615 at 1635.194 kb on + strand, within SM_b20615 at 1635.195 kb on - strand, within SM_b20615 at 1635.255 kb on + strand, within SM_b20615 at 1635.257 kb on + strand, within SM_b20615 at 1635.257 kb on + strand, within SM_b20615 at 1635.360 kb on + strand, within SM_b20615 at 1635.361 kb on - strand, within SM_b20615 at 1635.735 kb on + strand at 1635.735 kb on + strand at 1635.735 kb on + strand at 1635.736 kb on - strand at 1635.857 kb on + strand at 1635.858 kb on - strand at 1635.858 kb on - strand at 1636.429 kb on + strand, within SM_b20616 at 1636.431 kb on + strand, within SM_b20616 at 1636.749 kb on + strand at 1636.919 kb on + strand at 1636.967 kb on + strand at 1636.967 kb on + strand at 1637.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (N) remove 1,634,896 - SM_b20615 0.52 -0.3 1,634,897 + SM_b20615 0.52 -0.3 1,634,898 - SM_b20615 0.52 +0.3 1,634,994 + SM_b20615 0.57 +0.2 1,634,995 - SM_b20615 0.57 -0.0 1,635,012 - SM_b20615 0.58 -0.1 1,635,033 + SM_b20615 0.59 -0.5 1,635,033 + SM_b20615 0.59 +0.8 1,635,033 + SM_b20615 0.59 +0.0 1,635,194 + SM_b20615 0.68 +0.2 1,635,194 + SM_b20615 0.68 +0.3 1,635,195 - SM_b20615 0.68 +1.2 1,635,255 + SM_b20615 0.71 +0.6 1,635,257 + SM_b20615 0.72 +0.2 1,635,257 + SM_b20615 0.72 -0.0 1,635,360 + SM_b20615 0.77 -2.1 1,635,361 - SM_b20615 0.77 +0.3 1,635,735 + +0.3 1,635,735 + -1.3 1,635,735 + -0.7 1,635,736 - +0.2 1,635,857 + -2.7 1,635,858 - -1.4 1,635,858 - -0.6 1,636,429 + SM_b20616 0.66 -0.9 1,636,431 + SM_b20616 0.66 -3.0 1,636,749 + -4.0 1,636,919 + -3.7 1,636,967 + -1.4 1,636,967 + -0.7 1,637,743 + -0.3
Or see this region's nucleotide sequence