Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_739

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_737 and Pf1N1B4_738 overlap by 8 nucleotidesPf1N1B4_738 and Pf1N1B4_739 are separated by 25 nucleotidesPf1N1B4_739 and Pf1N1B4_740 are separated by 108 nucleotidesPf1N1B4_740 and Pf1N1B4_741 are separated by 86 nucleotides Pf1N1B4_737 - Electron transport complex protein RnfG, at 840,145 to 840,756 _737 Pf1N1B4_738 - Electron transport complex protein RnfE, at 840,749 to 841,339 _738 Pf1N1B4_739 - Endonuclease III (EC 4.2.99.18), at 841,365 to 842,003 _739 Pf1N1B4_740 - Leucyl-tRNA synthetase, at 842,112 to 842,291 _740 Pf1N1B4_741 - DNA-binding response regulator, LuxR family, at 842,378 to 843,004 _741 Position (kb) 841 842 843Strain fitness (log2 ratio) -2 -1 0 1 2at 840.377 kb on - strand, within Pf1N1B4_737at 840.377 kb on - strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.485 kb on + strand, within Pf1N1B4_737at 840.486 kb on - strand, within Pf1N1B4_737at 840.555 kb on + strand, within Pf1N1B4_737at 840.555 kb on + strand, within Pf1N1B4_737at 840.556 kb on - strand, within Pf1N1B4_737at 840.556 kb on - strand, within Pf1N1B4_737at 840.706 kb on + strandat 840.706 kb on + strandat 840.707 kb on - strandat 840.707 kb on - strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.754 kb on + strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.755 kb on - strandat 840.769 kb on - strandat 840.788 kb on - strandat 840.866 kb on + strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.867 kb on - strand, within Pf1N1B4_738at 840.879 kb on + strand, within Pf1N1B4_738at 840.879 kb on + strand, within Pf1N1B4_738at 840.879 kb on + strandat 840.879 kb on + strandat 840.880 kb on - strand, within Pf1N1B4_738at 840.880 kb on - strand, within Pf1N1B4_738at 840.933 kb on + strand, within Pf1N1B4_738at 841.013 kb on + strand, within Pf1N1B4_738at 841.273 kb on + strand, within Pf1N1B4_738at 841.274 kb on - strand, within Pf1N1B4_738at 841.281 kb on + strandat 841.288 kb on - strandat 841.440 kb on + strand, within Pf1N1B4_739at 841.441 kb on - strand, within Pf1N1B4_739at 841.441 kb on - strand, within Pf1N1B4_739at 841.542 kb on - strand, within Pf1N1B4_739at 841.587 kb on + strand, within Pf1N1B4_739at 841.587 kb on + strand, within Pf1N1B4_739at 841.588 kb on - strand, within Pf1N1B4_739at 841.648 kb on + strand, within Pf1N1B4_739at 841.649 kb on - strand, within Pf1N1B4_739at 841.675 kb on + strand, within Pf1N1B4_739at 841.876 kb on - strand, within Pf1N1B4_739at 841.876 kb on - strand, within Pf1N1B4_739at 841.918 kb on - strand, within Pf1N1B4_739at 841.918 kb on - strand, within Pf1N1B4_739at 841.977 kb on + strandat 841.978 kb on - strandat 841.978 kb on - strandat 841.978 kb on - strandat 841.978 kb on - strandat 841.978 kb on - strandat 842.069 kb on + strandat 842.071 kb on + strandat 842.071 kb on + strandat 842.072 kb on - strandat 842.094 kb on + strandat 842.095 kb on - strandat 842.105 kb on + strandat 842.106 kb on - strandat 842.111 kb on + strandat 842.111 kb on + strandat 842.112 kb on - strandat 842.112 kb on - strandat 842.140 kb on + strand, within Pf1N1B4_740at 842.140 kb on + strand, within Pf1N1B4_740at 842.140 kb on + strand, within Pf1N1B4_740at 842.140 kb on + strand, within Pf1N1B4_740at 842.141 kb on - strand, within Pf1N1B4_740at 842.141 kb on - strand, within Pf1N1B4_740at 842.287 kb on + strandat 842.287 kb on + strandat 842.287 kb on + strandat 842.287 kb on + strandat 842.288 kb on - strandat 842.296 kb on + strandat 842.296 kb on + strandat 842.296 kb on + strandat 842.296 kb on + strandat 842.297 kb on - strandat 842.324 kb on - strandat 842.327 kb on - strandat 842.327 kb on - strandat 842.327 kb on - strandat 842.382 kb on - strandat 842.459 kb on - strand, within Pf1N1B4_741at 842.459 kb on - strand, within Pf1N1B4_741at 842.461 kb on - strand, within Pf1N1B4_741at 842.461 kb on - strand, within Pf1N1B4_741at 842.461 kb on - strand, within Pf1N1B4_741at 842.461 kb on - strand, within Pf1N1B4_741at 842.638 kb on - strand, within Pf1N1B4_741at 842.638 kb on - strand, within Pf1N1B4_741at 842.638 kb on - strand, within Pf1N1B4_741at 842.643 kb on + strand, within Pf1N1B4_741at 842.664 kb on - strand, within Pf1N1B4_741at 842.905 kb on + strand, within Pf1N1B4_741at 842.950 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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840,377 - Pf1N1B4_737 0.38 -1.0
840,377 - Pf1N1B4_737 0.38 +0.6
840,485 + Pf1N1B4_737 0.56 +1.1
840,485 + Pf1N1B4_737 0.56 +1.0
840,485 + Pf1N1B4_737 0.56 -0.5
840,485 + Pf1N1B4_737 0.56 +0.2
840,486 - Pf1N1B4_737 0.56 +0.4
840,555 + Pf1N1B4_737 0.67 -0.3
840,555 + Pf1N1B4_737 0.67 -0.8
840,556 - Pf1N1B4_737 0.67 -2.2
840,556 - Pf1N1B4_737 0.67 -0.5
840,706 + +1.4
840,706 + -0.4
840,707 - -1.3
840,707 - +1.1
840,754 + -1.5
840,754 + -0.6
840,754 + +1.1
840,754 + +0.8
840,754 + -0.2
840,754 + +2.2
840,755 - -0.4
840,755 - -1.2
840,755 - +0.8
840,755 - -0.8
840,755 - -1.6
840,755 - -0.9
840,755 - -0.8
840,755 - -0.5
840,769 - -0.8
840,788 - -1.1
840,866 + Pf1N1B4_738 0.20 +0.8
840,867 - Pf1N1B4_738 0.20 -0.2
840,867 - Pf1N1B4_738 0.20 +1.8
840,867 - Pf1N1B4_738 0.20 -0.2
840,879 + Pf1N1B4_738 0.22 -0.1
840,879 + Pf1N1B4_738 0.22 -0.2
840,879 + +0.8
840,879 + +0.2
840,880 - Pf1N1B4_738 0.22 -1.3
840,880 - Pf1N1B4_738 0.22 +0.4
840,933 + Pf1N1B4_738 0.31 -1.0
841,013 + Pf1N1B4_738 0.45 -0.2
841,273 + Pf1N1B4_738 0.89 -1.8
841,274 - Pf1N1B4_738 0.89 -1.0
841,281 + +1.4
841,288 - +0.3
841,440 + Pf1N1B4_739 0.12 -0.5
841,441 - Pf1N1B4_739 0.12 -1.4
841,441 - Pf1N1B4_739 0.12 -1.8
841,542 - Pf1N1B4_739 0.28 +2.1
841,587 + Pf1N1B4_739 0.35 -1.7
841,587 + Pf1N1B4_739 0.35 -1.2
841,588 - Pf1N1B4_739 0.35 -0.8
841,648 + Pf1N1B4_739 0.44 +1.8
841,649 - Pf1N1B4_739 0.44 -0.2
841,675 + Pf1N1B4_739 0.49 -0.4
841,876 - Pf1N1B4_739 0.80 +0.2
841,876 - Pf1N1B4_739 0.80 -0.8
841,918 - Pf1N1B4_739 0.87 -0.9
841,918 - Pf1N1B4_739 0.87 +0.4
841,977 + -0.0
841,978 - -1.2
841,978 - -0.8
841,978 - +0.8
841,978 - -0.3
841,978 - +0.4
842,069 + -0.1
842,071 + -0.0
842,071 + -0.2
842,072 - -0.1
842,094 + -0.8
842,095 - -1.5
842,105 + -0.8
842,106 - -0.8
842,111 + +1.8
842,111 + -0.7
842,112 - +1.1
842,112 - +0.8
842,140 + Pf1N1B4_740 0.16 -0.6
842,140 + Pf1N1B4_740 0.16 +1.0
842,140 + Pf1N1B4_740 0.16 +1.3
842,140 + Pf1N1B4_740 0.16 +0.8
842,141 - Pf1N1B4_740 0.16 +1.4
842,141 - Pf1N1B4_740 0.16 +0.2
842,287 + +1.0
842,287 + +0.8
842,287 + -0.1
842,287 + +1.1
842,288 - -0.5
842,296 + +1.8
842,296 + -0.8
842,296 + +1.3
842,296 + -0.0
842,297 - +0.1
842,324 - +0.2
842,327 - -1.2
842,327 - -1.8
842,327 - -0.2
842,382 - -0.2
842,459 - Pf1N1B4_741 0.13 +0.1
842,459 - Pf1N1B4_741 0.13 -0.2
842,461 - Pf1N1B4_741 0.13 +0.3
842,461 - Pf1N1B4_741 0.13 +1.2
842,461 - Pf1N1B4_741 0.13 +1.8
842,461 - Pf1N1B4_741 0.13 +0.5
842,638 - Pf1N1B4_741 0.41 -0.8
842,638 - Pf1N1B4_741 0.41 +0.8
842,638 - Pf1N1B4_741 0.41 -0.5
842,643 + Pf1N1B4_741 0.42 -1.2
842,664 - Pf1N1B4_741 0.46 -1.2
842,905 + Pf1N1B4_741 0.84 -0.7
842,950 + -1.0

Or see this region's nucleotide sequence