Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_575 and Pf1N1B4_576 overlap by 6 nucleotides Pf1N1B4_576 and Pf1N1B4_577 are separated by 123 nucleotides
Pf1N1B4_575 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 668,183 to 669,700
_575
Pf1N1B4_576 - hypothetical protein, at 669,695 to 669,835
_576
Pf1N1B4_577 - Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative, at 669,959 to 670,903
_577
Position (kb)
669
670 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 668.809 kb on + strand, within Pf1N1B4_575 at 668.809 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.889 kb on + strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand, within Pf1N1B4_575 at 668.890 kb on - strand at 668.890 kb on - strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.893 kb on + strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.894 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 668.899 kb on - strand, within Pf1N1B4_575 at 669.060 kb on + strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.149 kb on - strand, within Pf1N1B4_575 at 669.166 kb on - strand, within Pf1N1B4_575 at 669.166 kb on - strand, within Pf1N1B4_575 at 669.202 kb on + strand, within Pf1N1B4_575 at 669.319 kb on - strand, within Pf1N1B4_575 at 669.376 kb on - strand, within Pf1N1B4_575 at 669.376 kb on - strand, within Pf1N1B4_575 at 669.547 kb on - strand, within Pf1N1B4_575 at 669.547 kb on - strand, within Pf1N1B4_575 at 669.547 kb on - strand at 669.631 kb on + strand at 669.631 kb on + strand at 669.632 kb on - strand at 669.632 kb on - strand at 669.632 kb on - strand at 669.632 kb on - strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.633 kb on + strand at 669.634 kb on - strand at 669.634 kb on - strand at 669.716 kb on + strand at 669.716 kb on + strand, within Pf1N1B4_576 at 669.790 kb on + strand, within Pf1N1B4_576 at 669.797 kb on + strand, within Pf1N1B4_576 at 669.797 kb on + strand, within Pf1N1B4_576 at 669.797 kb on + strand, within Pf1N1B4_576 at 669.798 kb on - strand, within Pf1N1B4_576 at 669.798 kb on - strand, within Pf1N1B4_576 at 669.798 kb on - strand, within Pf1N1B4_576 at 669.815 kb on + strand, within Pf1N1B4_576 at 669.815 kb on + strand, within Pf1N1B4_576 at 669.816 kb on - strand, within Pf1N1B4_576 at 669.816 kb on - strand, within Pf1N1B4_576 at 669.816 kb on - strand, within Pf1N1B4_576 at 669.878 kb on - strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.896 kb on + strand at 669.897 kb on - strand at 669.897 kb on - strand at 669.897 kb on - strand at 669.897 kb on - strand at 669.897 kb on - strand at 669.897 kb on - strand at 670.125 kb on - strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.133 kb on + strand, within Pf1N1B4_577 at 670.146 kb on + strand, within Pf1N1B4_577 at 670.146 kb on + strand, within Pf1N1B4_577 at 670.147 kb on - strand at 670.147 kb on - strand, within Pf1N1B4_577 at 670.147 kb on - strand, within Pf1N1B4_577 at 670.282 kb on - strand, within Pf1N1B4_577 at 670.282 kb on - strand, within Pf1N1B4_577 at 670.307 kb on + strand, within Pf1N1B4_577 at 670.307 kb on + strand, within Pf1N1B4_577 at 670.308 kb on - strand, within Pf1N1B4_577 at 670.308 kb on - strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.322 kb on + strand, within Pf1N1B4_577 at 670.323 kb on - strand at 670.323 kb on - strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.559 kb on + strand, within Pf1N1B4_577 at 670.560 kb on - strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577 at 670.783 kb on + strand, within Pf1N1B4_577
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 668,809 + Pf1N1B4_575 0.41 +0.4 668,809 + Pf1N1B4_575 0.41 +0.7 668,889 + -0.8 668,889 + Pf1N1B4_575 0.47 +0.7 668,889 + Pf1N1B4_575 0.47 +0.5 668,889 + Pf1N1B4_575 0.47 +1.7 668,889 + Pf1N1B4_575 0.47 +1.3 668,889 + Pf1N1B4_575 0.47 -1.8 668,889 + Pf1N1B4_575 0.47 +0.7 668,889 + Pf1N1B4_575 0.47 -0.3 668,889 + Pf1N1B4_575 0.47 -1.0 668,889 + Pf1N1B4_575 0.47 -0.5 668,889 + Pf1N1B4_575 0.47 +0.7 668,889 + Pf1N1B4_575 0.47 +0.9 668,889 + Pf1N1B4_575 0.47 +0.2 668,889 + Pf1N1B4_575 0.47 +0.4 668,889 + Pf1N1B4_575 0.47 +1.7 668,890 - Pf1N1B4_575 0.47 -1.0 668,890 - Pf1N1B4_575 0.47 -0.3 668,890 - Pf1N1B4_575 0.47 +0.7 668,890 - Pf1N1B4_575 0.47 +0.3 668,890 - Pf1N1B4_575 0.47 -0.7 668,890 - -0.8 668,890 - Pf1N1B4_575 0.47 +0.3 668,893 + Pf1N1B4_575 0.47 -1.3 668,893 + Pf1N1B4_575 0.47 +0.2 668,893 + Pf1N1B4_575 0.47 +0.7 668,893 + Pf1N1B4_575 0.47 +0.2 668,893 + Pf1N1B4_575 0.47 -0.1 668,894 - Pf1N1B4_575 0.47 -1.3 668,894 - Pf1N1B4_575 0.47 +0.7 668,894 - Pf1N1B4_575 0.47 -2.1 668,894 - +0.7 668,894 - Pf1N1B4_575 0.47 -1.1 668,894 - Pf1N1B4_575 0.47 +0.1 668,894 - Pf1N1B4_575 0.47 +0.7 668,894 - Pf1N1B4_575 0.47 +0.7 668,894 - Pf1N1B4_575 0.47 -1.1 668,894 - Pf1N1B4_575 0.47 -0.5 668,894 - Pf1N1B4_575 0.47 +0.0 668,894 - Pf1N1B4_575 0.47 -0.1 668,894 - Pf1N1B4_575 0.47 +1.1 668,899 - Pf1N1B4_575 0.47 -0.3 668,899 - Pf1N1B4_575 0.47 +1.4 668,899 - Pf1N1B4_575 0.47 -0.3 669,060 + Pf1N1B4_575 0.58 -0.4 669,149 - Pf1N1B4_575 0.64 +0.9 669,149 - Pf1N1B4_575 0.64 +1.3 669,149 - Pf1N1B4_575 0.64 -1.2 669,166 - Pf1N1B4_575 0.65 +0.0 669,166 - Pf1N1B4_575 0.65 -2.7 669,202 + Pf1N1B4_575 0.67 +0.7 669,319 - Pf1N1B4_575 0.75 -1.3 669,376 - Pf1N1B4_575 0.79 +0.4 669,376 - Pf1N1B4_575 0.79 +1.0 669,547 - Pf1N1B4_575 0.90 +0.3 669,547 - Pf1N1B4_575 0.90 +1.0 669,547 - -0.3 669,631 + -0.3 669,631 + +0.2 669,632 - +0.7 669,632 - +0.9 669,632 - -1.8 669,632 - -0.3 669,633 + -1.6 669,633 + -0.7 669,633 + -2.8 669,633 + -0.3 669,633 + +0.3 669,633 + -0.4 669,633 + +0.2 669,634 - -0.3 669,634 - -1.3 669,716 + -0.3 669,716 + Pf1N1B4_576 0.15 +0.0 669,790 + Pf1N1B4_576 0.67 -0.1 669,797 + Pf1N1B4_576 0.72 -1.7 669,797 + Pf1N1B4_576 0.72 -0.8 669,797 + Pf1N1B4_576 0.72 -2.1 669,798 - Pf1N1B4_576 0.73 -1.4 669,798 - Pf1N1B4_576 0.73 -0.7 669,798 - Pf1N1B4_576 0.73 -0.8 669,815 + Pf1N1B4_576 0.85 -1.1 669,815 + Pf1N1B4_576 0.85 -0.6 669,816 - Pf1N1B4_576 0.86 -2.3 669,816 - Pf1N1B4_576 0.86 -0.5 669,816 - Pf1N1B4_576 0.86 -0.8 669,878 - +0.7 669,896 + +2.7 669,896 + +0.7 669,896 + +2.1 669,896 + -0.1 669,896 + +0.1 669,896 + +0.0 669,896 + +0.2 669,896 + -1.1 669,896 + +0.2 669,896 + -0.6 669,897 - -0.3 669,897 - +1.0 669,897 - +0.0 669,897 - -0.3 669,897 - -0.8 669,897 - +0.1 670,125 - Pf1N1B4_577 0.18 -0.7 670,133 + Pf1N1B4_577 0.18 -1.8 670,133 + Pf1N1B4_577 0.18 +0.6 670,133 + Pf1N1B4_577 0.18 +0.7 670,146 + Pf1N1B4_577 0.20 +0.4 670,146 + Pf1N1B4_577 0.20 -0.6 670,147 - -0.3 670,147 - Pf1N1B4_577 0.20 -0.3 670,147 - Pf1N1B4_577 0.20 +0.7 670,282 - Pf1N1B4_577 0.34 -0.3 670,282 - Pf1N1B4_577 0.34 -1.0 670,307 + Pf1N1B4_577 0.37 +0.1 670,307 + Pf1N1B4_577 0.37 +0.0 670,308 - Pf1N1B4_577 0.37 -0.8 670,308 - Pf1N1B4_577 0.37 -1.8 670,322 + Pf1N1B4_577 0.38 -0.8 670,322 + Pf1N1B4_577 0.38 -0.5 670,322 + Pf1N1B4_577 0.38 -0.2 670,323 - -0.3 670,323 - Pf1N1B4_577 0.39 -1.8 670,559 + Pf1N1B4_577 0.63 -0.5 670,559 + Pf1N1B4_577 0.63 -0.4 670,559 + Pf1N1B4_577 0.63 +0.0 670,560 - Pf1N1B4_577 0.64 +0.0 670,783 + Pf1N1B4_577 0.87 +0.7 670,783 + Pf1N1B4_577 0.87 -0.3 670,783 + Pf1N1B4_577 0.87 -0.4
Or see this region's nucleotide sequence