Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5747

Experiment: 5-Hydroxymethylfurfural 2.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5746 and Pf1N1B4_5747 are separated by 132 nucleotidesPf1N1B4_5747 and Pf1N1B4_5748 are separated by 30 nucleotidesPf1N1B4_5748 and Pf1N1B4_5749 are separated by 262 nucleotides Pf1N1B4_5746 - FIG139552: Putative protease, at 187,631 to 188,626 _5746 Pf1N1B4_5747 - Molybdopterin biosynthesis protein MoeA, at 188,759 to 189,538 _5747 Pf1N1B4_5748 - hypothetical protein, at 189,569 to 189,727 _5748 Pf1N1B4_5749 - Molybdenum cofactor biosynthesis protein MoaB, at 189,990 to 190,544 _5749 Position (kb) 188 189 190Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 187.788 kb on + strand, within Pf1N1B4_5746at 187.788 kb on + strandat 188.085 kb on + strand, within Pf1N1B4_5746at 188.086 kb on - strandat 188.123 kb on + strand, within Pf1N1B4_5746at 188.123 kb on + strand, within Pf1N1B4_5746at 188.124 kb on - strand, within Pf1N1B4_5746at 188.124 kb on - strandat 188.180 kb on + strand, within Pf1N1B4_5746at 188.181 kb on - strand, within Pf1N1B4_5746at 188.241 kb on + strand, within Pf1N1B4_5746at 188.242 kb on - strand, within Pf1N1B4_5746at 188.322 kb on + strand, within Pf1N1B4_5746at 188.322 kb on + strand, within Pf1N1B4_5746at 188.322 kb on + strand, within Pf1N1B4_5746at 188.553 kb on + strandat 188.554 kb on - strandat 188.944 kb on - strand, within Pf1N1B4_5747at 189.009 kb on - strand, within Pf1N1B4_5747at 189.316 kb on - strand, within Pf1N1B4_5747at 189.378 kb on + strandat 189.378 kb on + strand, within Pf1N1B4_5747at 189.378 kb on + strand, within Pf1N1B4_5747at 189.379 kb on - strand, within Pf1N1B4_5747at 189.593 kb on - strand, within Pf1N1B4_5748at 190.158 kb on - strand, within Pf1N1B4_5749at 190.379 kb on - strand, within Pf1N1B4_5749at 190.415 kb on + strand, within Pf1N1B4_5749at 190.415 kb on + strand, within Pf1N1B4_5749at 190.415 kb on + strand, within Pf1N1B4_5749at 190.416 kb on - strand, within Pf1N1B4_5749at 190.416 kb on - strand, within Pf1N1B4_5749at 190.416 kb on - strand, within Pf1N1B4_5749

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM
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187,788 + Pf1N1B4_5746 0.16 -1.1
187,788 + +1.4
188,085 + Pf1N1B4_5746 0.46 +0.8
188,086 - +0.4
188,123 + Pf1N1B4_5746 0.49 -0.6
188,123 + Pf1N1B4_5746 0.49 -0.3
188,124 - Pf1N1B4_5746 0.49 +0.4
188,124 - -1.9
188,180 + Pf1N1B4_5746 0.55 -0.8
188,181 - Pf1N1B4_5746 0.55 +0.0
188,241 + Pf1N1B4_5746 0.61 +0.8
188,242 - Pf1N1B4_5746 0.61 -0.0
188,322 + Pf1N1B4_5746 0.69 +2.0
188,322 + Pf1N1B4_5746 0.69 -1.1
188,322 + Pf1N1B4_5746 0.69 -3.0
188,553 + -0.3
188,554 - +0.4
188,944 - Pf1N1B4_5747 0.24 -1.7
189,009 - Pf1N1B4_5747 0.32 -1.1
189,316 - Pf1N1B4_5747 0.71 -0.6
189,378 + +1.2
189,378 + Pf1N1B4_5747 0.79 -0.7
189,378 + Pf1N1B4_5747 0.79 +1.4
189,379 - Pf1N1B4_5747 0.79 +0.2
189,593 - Pf1N1B4_5748 0.15 -0.4
190,158 - Pf1N1B4_5749 0.30 -0.0
190,379 - Pf1N1B4_5749 0.70 -0.7
190,415 + Pf1N1B4_5749 0.77 -0.3
190,415 + Pf1N1B4_5749 0.77 +1.3
190,415 + Pf1N1B4_5749 0.77 +0.0
190,416 - Pf1N1B4_5749 0.77 -0.8
190,416 - Pf1N1B4_5749 0.77 +1.1
190,416 - Pf1N1B4_5749 0.77 -0.6

Or see this region's nucleotide sequence