Experiment: 5-Hydroxymethylfurfural 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5643 and Pf1N1B4_5644 are separated by 9 nucleotides Pf1N1B4_5644 and Pf1N1B4_5645 are separated by 52 nucleotides Pf1N1B4_5645 and Pf1N1B4_5646 overlap by 6 nucleotides Pf1N1B4_5646 and Pf1N1B4_5647 are separated by 197 nucleotides Pf1N1B4_5647 and Pf1N1B4_5648 are separated by 133 nucleotides
Pf1N1B4_5643 - Amino acid ABC transporter, permease protein, at 72,904 to 73,566
_5643
Pf1N1B4_5644 - amino acid ABC transporter, permease protein, at 73,576 to 74,238
_5644
Pf1N1B4_5645 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 74,291 to 75,136
_5645
Pf1N1B4_5646 - hypothetical protein, at 75,131 to 75,280
_5646
Pf1N1B4_5647 - hypothetical protein, at 75,478 to 75,927
_5647
Pf1N1B4_5648 - transcriptional regulator, MerR family, at 76,061 to 76,465
_5648
Position (kb) 74
75
76 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.332 kb on - strand, within Pf1N1B4_5643 at 73.332 kb on - strand, within Pf1N1B4_5643 at 73.352 kb on - strand, within Pf1N1B4_5643 at 73.562 kb on + strand at 73.563 kb on - strand at 73.563 kb on - strand at 73.646 kb on + strand, within Pf1N1B4_5644 at 73.647 kb on - strand, within Pf1N1B4_5644 at 73.647 kb on - strand, within Pf1N1B4_5644 at 73.713 kb on - strand, within Pf1N1B4_5644 at 73.715 kb on + strand, within Pf1N1B4_5644 at 73.716 kb on - strand, within Pf1N1B4_5644 at 73.716 kb on - strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.829 kb on + strand, within Pf1N1B4_5644 at 73.830 kb on - strand, within Pf1N1B4_5644 at 73.940 kb on + strand, within Pf1N1B4_5644 at 73.991 kb on - strand, within Pf1N1B4_5644 at 74.009 kb on + strand, within Pf1N1B4_5644 at 74.010 kb on - strand, within Pf1N1B4_5644 at 74.060 kb on + strand, within Pf1N1B4_5644 at 74.060 kb on + strand at 74.060 kb on + strand, within Pf1N1B4_5644 at 74.231 kb on + strand at 74.232 kb on - strand at 74.232 kb on - strand at 74.248 kb on + strand at 74.248 kb on + strand at 74.249 kb on - strand at 74.249 kb on - strand at 74.367 kb on + strand at 74.461 kb on - strand, within Pf1N1B4_5645 at 74.461 kb on - strand at 74.523 kb on + strand, within Pf1N1B4_5645 at 74.524 kb on - strand at 74.584 kb on - strand, within Pf1N1B4_5645 at 74.656 kb on - strand, within Pf1N1B4_5645 at 74.721 kb on + strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.722 kb on - strand, within Pf1N1B4_5645 at 74.999 kb on - strand, within Pf1N1B4_5645 at 75.188 kb on - strand, within Pf1N1B4_5646 at 75.577 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.625 kb on + strand, within Pf1N1B4_5647 at 75.626 kb on - strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.627 kb on + strand, within Pf1N1B4_5647 at 75.647 kb on - strand, within Pf1N1B4_5647 at 75.712 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 75.869 kb on + strand, within Pf1N1B4_5647 at 76.062 kb on + strand at 76.062 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 2.5 mM remove 73,332 - Pf1N1B4_5643 0.65 +1.1 73,332 - Pf1N1B4_5643 0.65 -0.7 73,352 - Pf1N1B4_5643 0.68 -1.1 73,562 + -1.0 73,563 - -0.5 73,563 - -0.3 73,646 + Pf1N1B4_5644 0.11 +0.4 73,647 - Pf1N1B4_5644 0.11 +0.9 73,647 - Pf1N1B4_5644 0.11 +1.2 73,713 - Pf1N1B4_5644 0.21 +0.4 73,715 + Pf1N1B4_5644 0.21 +1.4 73,716 - Pf1N1B4_5644 0.21 -1.6 73,716 - Pf1N1B4_5644 0.21 -0.6 73,829 + Pf1N1B4_5644 0.38 +0.3 73,829 + Pf1N1B4_5644 0.38 +2.4 73,829 + Pf1N1B4_5644 0.38 -1.9 73,830 - Pf1N1B4_5644 0.38 +1.4 73,940 + Pf1N1B4_5644 0.55 +1.0 73,991 - Pf1N1B4_5644 0.63 +1.3 74,009 + Pf1N1B4_5644 0.65 +0.4 74,010 - Pf1N1B4_5644 0.65 -0.1 74,060 + Pf1N1B4_5644 0.73 -0.9 74,060 + +0.4 74,060 + Pf1N1B4_5644 0.73 +0.1 74,231 + +0.9 74,232 - -0.3 74,232 - -0.9 74,248 + -0.1 74,248 + +0.0 74,249 - +0.3 74,249 - -0.8 74,367 + -0.3 74,461 - Pf1N1B4_5645 0.20 -0.6 74,461 - +0.7 74,523 + Pf1N1B4_5645 0.27 -0.2 74,524 - +0.0 74,584 - Pf1N1B4_5645 0.35 -1.1 74,656 - Pf1N1B4_5645 0.43 -0.6 74,721 + Pf1N1B4_5645 0.51 -1.1 74,722 - Pf1N1B4_5645 0.51 -0.1 74,722 - Pf1N1B4_5645 0.51 +0.4 74,999 - Pf1N1B4_5645 0.84 -1.7 75,188 - Pf1N1B4_5646 0.38 +0.0 75,577 + Pf1N1B4_5647 0.22 -0.1 75,625 + Pf1N1B4_5647 0.33 -0.9 75,625 + Pf1N1B4_5647 0.33 +0.8 75,625 + Pf1N1B4_5647 0.33 +1.3 75,626 - Pf1N1B4_5647 0.33 -1.1 75,627 + Pf1N1B4_5647 0.33 -0.1 75,627 + Pf1N1B4_5647 0.33 +0.4 75,627 + Pf1N1B4_5647 0.33 +1.2 75,627 + Pf1N1B4_5647 0.33 -0.1 75,627 + Pf1N1B4_5647 0.33 -0.9 75,647 - Pf1N1B4_5647 0.38 -0.6 75,712 + Pf1N1B4_5647 0.52 +0.1 75,869 + Pf1N1B4_5647 0.87 +1.0 75,869 + Pf1N1B4_5647 0.87 +0.0 76,062 + +0.9 76,062 + -0.0
Or see this region's nucleotide sequence